ESTIMATION OF PLOIDY AND HYBRIDIZATION STATU8 BY ERYTHROCYTE AND NUCLEUS SIZE ANALYSIS IN CARPS
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Authors
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Vidya R. Nair
Central Institute of Fisheries Education, Versova, Mumbai - 400 061
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Gopalkrishna Gopalkrishna
Central Institute of Fisheries Education, Versova, Mumbai - 400 061
Abstract
The present experiment was designed to observe whether the nuclear
volume and area are affected by the ploidy and hybrid status of the individual.
Polyploidy was induced by heat shock treatment given at 44 ± 0.5°C for 30
seconds and 45 seconds which was found to be most effective (64.7%) for
induction of triploidy in Cyprinus carpio. Cell and nuclear volume and cell
and nuclear area varied significantly in triploid fishes as compared to those
of controls. Triploid fishes showed significantly higher growth compared to
diploid counterparts. It was also observed that catla x rohu hybrid and its
parents showed significant difference in the nuclear volume and area of their
erythrocytes. Except nuclear volume, all the parameters were significantly
different between catla and catla x rohu hybrid. The hybrids showed a closer
relationship with catla as compared to rohu.
Key words: Ploidy, hybridization, nucleus size
