RAPID DETECTION OF WHITE SPOT SYNDROME VIRUS (WSSV) OF PENAEUS MONODON BY LATEX AGGLUTINATION TEST USING MONOCLONAL ANTIBODIES
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Authors
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M. Makesh
Centra/Institute ofFislzeries Education. Fisheries Universizv Road.
Versova, Mumbai- 400 06 I. India
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A. Koteeswaran
Department a_{ Veterinary Microbiology. Madras Veterinmy College,
Chennai- 600 007, India
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N. Daniel Joy Chandran
Department a_{ Veterinary Microbiology. Madras Veterinmy College,
Chennai- 600 007, India
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B. Murali Manohar
Department a_{ Veterinary Microbiology. Madras Veterinmy College,
Chennai- 600 007, India
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V. Ramasamy
Department a_{ Veterinary Microbiology. Madras Veterinmy College,
Chennai- 600 007, India
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A. Chandramohan
Department a_{ Veterinary Microbiology. Madras Veterinmy College,
Chennai- 600 007, India
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R. P. Raman
Centra/Institute ofFislzeries Education. Fisheries Universizv Road.
Versova, Mumbai- 400 06 I. India
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S. C. Mukherjee
Centra/Institute ofFislzeries Education. Fisheries Universizv Road.
Versova, Mumbai- 400 06 I. India
Abstract
Latex beads were sensitized with monoclonal antibodies (!\'lAb) raised
against VP28 of WSSV. The optimum concentration of l\1Ab required to
sensitize the latex beads was 125 J.Ig/ml. The sensitized latex beads ,were used to
detect WSSV from PCR-positive stomach tissue homogenates obtained from
infected shrimp. Stomach tissue homogenates from \VSSV-infected shrimp
agglutinated the sensitized latex beads within I 0 minutes, while uninfected
samples did not produce any agglutination, although non-specific
agglutinations were observed in some samples. The analytical sensitivity,
analytical specificity, diagnostic sensitivity and diagnostic specificity of the
(LAT) agglutination test were assessed. The analytical sensitivity of the test
was 40 ng of purified WSSV (2 J.Iglml). The sensitized latex beads did not
agglutinate with normal shrimp tissue or MBV-infected tissue homogenate.
The test has a diagnostic sensitivity of 70 and 45o/o, respectively, compared to
single-step and nested PCR. The diagnostic specificity of the test was 824%. This
test is a simple and rapid on-farm test which can be used to corroborate clinical
signs for the detection ofWSSVin grow-out ponds.
Keywords: PenaerL-,' monodon, white spot syndrome virus, monoclonal
antibodies, latex agglutination test
