Micropropagation of Malaxis acuminata D. Don: A Rare Orchid of High Therapeutic Value
Abstract views: 437 / PDF downloads: 610
Regeneration potential of pseudobulb segments of Malaxis acuminata, procured from in vitro grown cultures, was analysed in Mitra et al., 1976 medium and its combinations with growth adjuncts. Juvenility of the tissues and chemical stimulus emerged as important factors in eliciting response in the explants. The explants from relatively older pseudobulbs (greater than 0.5cm in length) remained recalcitrant to regeneration whereas those representing younger ones (less than 0.5cm in length) responded positively. The response frequency, pathway of regeneration, and time taken for their development into complete plantlets was directly related to the growth stimulus in the nutrient regime. Shoot buds were induced in an individual treatment with growth regulators (BAP/NAA; 1mg L-1 each) whereas their combination [BAP (1.0 mg L-1) + NAA (1.0 mg L-1)] promoted protocorm-like body formation in the explants. Additional activated charcoal invariably proved beneficial in accelerating the morphogenetic processes leading to plantlet development. NAA (1 mg L-1) impaired the response frequency and delayed subsequent morphogenetic processes leading to plantlet development. BAP+NAA (1.0 mg L-leach)+AC (2 g L-1) was used best for early initiation, highest regeneration frequency, proliferation of protocorm-like bodies (PLB), and plantlet development. Plantlets were transferred to clay pots containing potting mixture (sand, soil, leaf compost) in the ratio of 1:1:1. Nearly 70-80% of plantlets survival was recorded. Histological investigation confirmed that neoformations were dermal and multicellular in origin.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 Unported License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).