Estimation of rosmarinic acid content of genetically stable clones of Ocimum basilicum L. (sweet basil) regenerated through in vitro shoot bud multiplication and root culture
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Authors
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Trayee Biswas
Rishi Bankim Chandra College, West Bengal State University, West Bengal, India
Keywords:
6-benzylaminopurine, genomic stability, in vitro systems, rosmarinic acidAbstract
Abstract Ocimum basilicum L. (Sweet Basil) of Lamiaceae is an aromatic annual herb of high pharmaceutical importance. The genus contains rosmarinic acid and its derivatives which have been reported to have antioxidant, anti-HIV and anti-inflammatory activity. Rapid in vitro propagation of the plant, by direct shoot bud multiplication is described. Murashige and Skoog’s basal medium (MS) supplemented with 1.5 mg/l 6-benzylaminopurine (BAP) in combination with 0.2 mg/l α-napthaleneacetic acid (NAA) was found to be effective for inducing multiple shoots (17.0±0.837) from the apical bud explants. The shoots were rooted in half strength of basal medium and roots were used as explants, to established in vitro root cultures. Maximum average rosmarinic acid content 2.66±0.07 % of dry weight obtained from roots cultured in the liquid one fourth strength of MS medium supplemented with 1.0 mg/l NAA and 10.0 mg/l putrescine The genomic stability of the regenerates was found to be maintained when the cytological status was verified using somatic chromosome analysis. The present investigation revealed superiority of in vitro systems over in vivo plants for production of rosmarinic acid.
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