Quantification of β-1,3-D-glucan in two wild edible mushrooms of Andhra Pradesh


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Authors

  • Isha Sai Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Anantapur Campus, 515001, AP, India
  • R Basavaraju Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, 515134, AP, India

Abstract

Mushroom derived β-glucan polysaccharides are known for their activity as immunomodulators and anticarcinogenic agents. In the present work, specific colorimetric method for β-1,3-D-glucan quantification was used. This method is based on interaction of a triple helix tertiary structure of β-1,3-D-glucan with congo red dye and was detected by bathochromic shift from 488 to 512nm (>20nm) in UV-VIS spectrophotometer. Two different methods (method-1 and method-2) were used to extract the polysaccharide in various fractions of mushroom samples. The results showed that the total β-1,3-glucan content was found to be more in both the mushrooms using extraction method-2. Termitomyces heimii was found to possess high amount of total β-1,3-D-glucan (43.55mg/g dry weight) compared to Podaxis pistillaris (9.89mg/g dry weight).

Author Biography

  • Isha Sai, Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Anantapur Campus, 515001, AP, India

    Associate Professor

    Department of Biosciences

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2020-10-09

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2021-10-11

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Quantification of β-1,3-D-glucan in two wild edible mushrooms of Andhra Pradesh. (2021). Mushroom Research, 29(2). https://epubs.icar.org.in/index.php/MR/article/view/105805