Quantification of β-1,3-D-glucan in two wild edible mushrooms of Andhra Pradesh

Abstract views: 166 / PDF downloads: 61


  • Isha Sai Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Anantapur Campus, 515001, AP, India
  • R Basavaraju Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, 515134, AP, India


Mushroom derived β-glucan polysaccharides are known for their activity as immunomodulators and anticarcinogenic agents. In the present work, specific colorimetric method for β-1,3-D-glucan quantification was used. This method is based on interaction of a triple helix tertiary structure of β-1,3-D-glucan with congo red dye and was detected by bathochromic shift from 488 to 512nm (>20nm) in UV-VIS spectrophotometer. Two different methods (method-1 and method-2) were used to extract the polysaccharide in various fractions of mushroom samples. The results showed that the total β-1,3-glucan content was found to be more in both the mushrooms using extraction method-2. Termitomyces heimii was found to possess high amount of total β-1,3-D-glucan (43.55mg/g dry weight) compared to Podaxis pistillaris (9.89mg/g dry weight).

Author Biography

  • Isha Sai, Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Anantapur Campus, 515001, AP, India

    Associate Professor

    Department of Biosciences


Ana Villares, (2013). Polysaccharides from the edible mushroom Calocybe gambosa: structure and chain conformation of a (1ï‚®4), (1ï‚®6)-linked glucan, Carbohydrate Research. 375:153-157.

Bohn, J. A., and BeMiller, J. N. (1995). (1→3)-β-glucans as biological response modifiers: A review of structure-functional activity relationships. Carbohydrate Polymers, 28: 3-14.

Borchers, A. T., Keen, C. L., and Gershwin, M. E. (2004). Mushrooms, tumors, and immunity: An update. Experimental Biology and Medicine, 229: 393-406.

Dong, C. H., and Yao, Y. J. (2008). In vitro evaluation of antioxidant activities of aqueous extracts from natural and cultured mycelia of Cordyceps sinensis. LWT- Food Science and Technology, 41: 669-677.

Falch, B. H., Espevik, T., Ryan, L., and Stokke, B. T. (2000). The cytokine stimulating activity of (1→3)-β-glucans is dependent on the triple helix conformation. Carbohydrate Research, 329: 587-596.

Kozarski, M., Klaus, A., Niksic, M., Jakovljevic, D., Helsper, J.P.F.G.and Griensven, LJLDV. (2011). Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. Food Chemistry, 129:1667-1675.

Lull, C., Wichers, H. J., and Savelkoul, H. F. (2005). Antiinflammatory and immunomodulating properties of fungal metabolites. Mediators Inflammation, 63-80.

Magnelli, P., Cipollo, J. F., and Abeijon, C., (2002). A refined method for the department of Saccharomyces cerevisiae cell wall composition and β-1,6-glucan fine structure. Analytical Biochemistry, 301: 136-150.

Mizuno, T., Saito, H., Nishitoba, T., and Kawagishi, H. (1995). Antitumor active substances from mushrooms. Food Reviews International, 11: 23-61.

Moradali, M. F., Mostafavi, H., Ghods, S., and Hedjaroude, G. A. (2007). Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). International Immunopharmacology, 7: 701-724.

Nitschke, J., Modick, H., Busch, E., Rekowski, R. W. V., Altenbach, H., and Molleken, H., (2011). A new colorimetric method to quantify β-1,3-1,6-glucans in comparison with total β-1,3-glucans in edible mushrooms, Food Chemistry, 127: 791-796.

Ogawa, K., Tsurugi, J., and Watanabe, T. (1972). Complex of gel-forming β-1,3-D-glucan with congo-red in alkaline solutions. Chemistry Letters by the Chemical Society of Japan, 689: 692.

Ogawa, K., Dohmaru, T., and Yui, T., (1993). Dependence of complex formation of (13) –β-D-glucan with Congo red on temperature in alkaline solution, Bioscience, Biotechnology and Biochemistry, 58(10): 1870-1872.

Ooi, V. E. and Liu, F. (2000). Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Current Medicinal Chemistry, 7: 715-729.

Ooi, V. E. C. and Liu, F. (1999). A review of pharmacological activities of mushroom polysaccharides. International Journal of Medicinal Mushrooms, 1: 195-206.

Palacios, I., Gracia, A., Guillamon, E., and Villares, A. (2012). Novel isolation of water-soluble polysaccharides from fruiting bodies of Pleurotus ostreatus mushrooms. Carbohydrate Research, 358:72-77.

Semedo, M.C., Karmali, A., and Fonseca, L., (2015). A high throughput colorimetric assay of β-1,3-D-glucans by Congo red dye. Journal of Microbiological Methods, 109: 140-148.

Sullivan, R., Smith, J. E., and Rowan, N. J. (2006). Medicinal mushrooms and cancer therapy -Translating a traditional practice into Western medicine. Perspectives in Biology and Medicine, 49: 159-170.

Thetsrimuang, C., Khammuang. S., Chiablaem, K., Srisomsap, C. and Sarnthima, R. (2011). Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév. Food Chemistry, 128: 634-639.

Wasser, S. P. (2002). Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Applied Microbiology and Biotechnology, 60: 258-274.

Wood, P. J. (1980). Specificity in the interaction of direct dyes with polysaccharides. Carbohydr. Res., 85: 271–287. http://dx.doi.org/10.1016/S0008-6215(00)84676-5.

Zekovic, D. B., Kwiatkowski, S., Vrvic, M. M., Jakovljevic, D., and Moran, C. A. (2005). Natural and modified (1→3)-β-D-glucans in health promotion and disease alleviation. Critical Reviews in Biotechnology, 25: 205-230.

Zhang, M., Cui, S. W., Cheung, P. C. K. and Wang, Q. (2007). Polysaccharides from mushrooms: A review on their isolation process, structural characteristics and antitumor activity. Trends in Food Science and Technology, 18: 4-19.









How to Cite

Quantification of β-1,3-D-glucan in two wild edible mushrooms of Andhra Pradesh. (2021). Mushroom Research, 29(2). https://epubs.icar.org.in/index.php/MR/article/view/105805