Lignolytic Enzymes (Laccase and Peroxidase) production by Pink Oyster Mushroom, Pleurotus djamor
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Authors
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Swati Rose Toppo
Atal Bihari Vajpayee Vishwavidyalaya Bilaspur, C.G.
Keywords:
Biogas digester, Lignolytic enzyme, oyster mushroom, Pleurotus djamorAbstract
This research work attempts the use of biogas digester liquid (BDL) as an inducer for lignolytic enzyme activity by Pleurotus djamor when grown in SSF culture condition. BDL rich in polyphenols and micronutrients can induce lignin degrading enzymes such as laccase and peroxidase. Spraying 100 ml BDL/bag (1kg paddy straw)/day was found to increase yields of Pleurotus djamor in SSF and also reduced the time for various stages of mushroom growth such as pin head formation, fruiting body formation and first flush by 3-5d. Of the 100ml BDL sprayed 70ml was collected as leachate and this showed highlignolytic enzyme (Laccase 150 U/L and Peroxidase 160U/L) activities in SSF and the enzyme activity coincided with the specific mushroom growth stages.
References
Alfarra HY, Hasali NHM and Omar MN (2013).Alignolytic Fungi with Laccase Activity isolated from Malaysian local environment for phytochemical transformation purposes. Int. Res. J. Biological Sci.2 (2), 51-54
An Q, Wu X J, Han M L, Cui BK, He SH, Dai YC, and Si J (2016a). “Sequential solid-state and submerged cultivation of white rot fungus Pleurotusostreatuson lignocellulosic biomass for the activity of lignocellulolytic enzymes,†BioResources.11 (4), 8791-8805.DOI: 10.15376/biores.11.4.8791-8805
Atila F (2017). Evaluation of suitability of various agro-wastes for productivity of Pleurotus djamor, Pleurotus citrinopileatus and Pleurotus eryngii mushrooms. Journal of Experimental Agriculture International 17(5): 1-11.
Cavallazzi1 JRP, Kasuya MC and Soares MA (2005). Screening of inducers for laccase production by Lentinula edodes in liquid medium. Brazilian Journal of Microbiology. 36:383-387
Chanakya HN, Malayil S and Vijayalakshmi C (2015). Cultivation of Pleurotus spp. on a combination of anaerobically digested plant material and various agro-residues. Energy Sustainable Dev. 27, 84–92.
Chesson A (1978).The Maceration of Linen Flax under anaerobic conditions.Journal of Applied Bacteriology. 45,219-230
da Luz JMR, Nunes MD, Paes SA, Torres DP, da Silva MCS, Kasuya MCM (2012). Lignocellulolytic enzyme production of Pleurotus Ostreatus growth in agro industrial wastes. Brazilian Journal of Microbiology, 1508-1515
Ganguli NK and Chanakya HN (1994). Mushroom cultivation on spent biomass from biogas plant. Curr. Sci. India 66, 70–73.
Gupta D, Chauhan P, Suman BC and Thakur M. (2014). Effect of different solid and liquid culture media on the growth of pink oyster mushroom (Pleurotus djamor var. roseus). Ind.J.Mushrooms. 32(1):18-21.
Kuhara F and Papinutti L (2014).Optimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers. Rev Argent Microbiol.46 (2):144-149
MalayilS, Ashwath R and Chanakya HN (2016a).Utilization of biogas digester liquid for higher mushroom yields. Proc. Environ. Sci. 35, 781–784.
Malayil S, Chanakya HN, Ashwath R (2016b). Biogas digester liquid—a nutrient supplement for mushroom cultivation. Environmental Nanotechnology, Monitoring & Management. 6: 24–31
Malayil S, Chanakya HN, Ashwath R and Suresh H (2017).Biogas digester liquid as a supplement for higher yields of Hypsizgus ulmarius. Environmental Technology & Innovation 8: 269–281
Mazumder S, Basu SK and Mukherjee M (2009). Laccase production in solid-state and submerged fermentation by Pleurotus ostreatus. Eng. Life Sci. 2009, 9, No. 1, 45–52
Munk L, Sitarz AK, Kalyani DC, Mikkelsen JD, Meyer AS (2015) Can laccases catalyze bond cleavage in lignin? Biotechnology Advances. 33 (2015) 13–24
Periasamy K and Natrajan K (2004).Role of lignocellulosic enzymes during basidiomata production by Pleurotus djamor var roseus. Indian journal of Biotechnology. 3: 577-583
Sadasivam S and Manickam A (1996). Biochemical Methods. ISBN: 81-2224-0976-8.
Salmones D and Mata G (2015). Laccase production by Pleurotus djamor in agar media and during cultivation on wheat straw. Revista Mexicana de MicologÃa. 42: 17-23
Singh S, Singh G, Kumar V, Kumar B and Kumar A (2017) Assessment of Different Organic Supplements (pulses flour) on Growth and Yield of Oyster Mushrooms (Pleurotusdjamor) Int. J. Pure App. Biosci. 5 (2): 101-106 DOI: http://dx.doi.org/10.18782/2320-7051.2819
Thiribhuvanamala G, Kalaiselvi G, Parthasarathy S and Anusha B (2017a). Induction of lignolytic enzyme activities in different agro residues by the white rot fungi, Pleurotus sajar-caju. International Journal of Chemical Studies 5(2): 89-94
Thiribhuvanamala G, Kalaiselvi G, Parthasarathy S, Madhavan S and Prakasam V (2017b). Extracellular secretion of lignocellulolytic enzymes by diverse white rot basidiomycetes fungi, Annals of Phytomedicine 6(1): 20-29
Toppo, S.R. and Chandravanshi, D (2018). Yield performance of Pleurotus florida on various agro-industrial wastes as growing substrate. Mushroom Research 27 (1) : 55-61
YounHD, Hah YC and Kang SO (1995). Role of lactase in lignin degradation by white-rot fungi. FEMS Microbiology Letters 132(3): 183- 188 https://doi.org/10.1111/j.1574-6968.1995.tb07831.x
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