In vitro evaluation for optimum growth conditions of shiitake mushroom (Lentinula edodes)


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Authors

  • Inkresh Kumar Verma Department of Plant Pathology, Dr. Rajendra Prasad Central Agricultural University, Pusa-848125, Samastipur, Bihar, India
  • Priya Singh Department of Plant Pathology, Dr. Rajendra Prasad Central Agricultural University, Pusa-848125, Samastipur, Bihar, India
  • Daya ram Department of Plant Pathology, Dr. Rajendra Prasad Central Agricultural University, Pusa-848125, Samastipur, Bihar, India

Abstract

The mushrooms are commercially cultivated for its nutritional and medicinal importance. Among allthe cultivated mushrooms, shiitake holds a special place due to its medicinal importance. The currentstudy was undertaken to explore the best media among potato dextrose agar (PDA), sawdust extractagar (SDEA), wheat straw extract agar (WSEA), paddy straw extract agar (PSEA), malt extract agar(MEA), wheat straw extract agar + paddy straw extract agar (WSEA + PSEA) in 1:1, maize strawextract agar (MSEA) and litchi leaf extract agar (LLEA) media. The potato dextrose agar media wasfound best among all the tested media at 7 and 14 days after inoculation. However, maize straw extractagar and paddy straw extract agar were the least effective media at 7 and 14 DAI. The 25°C temperaturewas most preferred among various temperature regimes of 15°C, 20°C, 25°C and 30°C while 15°Cwas least preferred temperature for mycelial proliferation of the shiitake.

References

Annepu, S.K., V.P. Sharma, A. Barh, S.Kumar, M. Shirur and S. Kamal. 2019. Effectsof genotype and growing substrate on bio efficiencyof gourmet and medicinal mushroom, Lentinula edodes (Berk.) Pegler. Bangladesh Journal of Botany 48(1): 129-138.

Annepu, S.K., V.P. Sharma, S. Kumar, A.Barh, S. Banyal and S. Kamal. 2018. Enzyme profile of shiitake mushroom strains grown on wheat straw. Indian Journal of Horticulture 75(3): 475-481. DOI : 10.5958/0974-0112.2018.00080.4

Bilay, V.T., E.F. Solomko and A.S. Buchalo.2000. Growth of edible and medicinalmushroom on commercial agar media. Science and Cultivation of Edible Fungi. Proceedings of 15th International Congress on theScience and Cultivation of Edible Fungi,Maastricht, Netherlands.

Chang, S.T. and P.G. Miles. 1992. Mushroom biology - a new discipline. The Mycologist 6:64-65.

Imtiaj, A., C. Jayasinghe, G.W. Lee and T.S.Lee. 2008. Comparative study of environmentaland nutritional factors on the mycelial growthof edible mushrooms. Journal of Culture collections 6: 97-105.

Kalmis, E. and F. Kalyoncu. 2006. Variationsin the isolates obtained from basidiospores ofcommercial Mushroom Lentinula edodes (Shiitake). International Journal of Science and Technology 1(2): 99-103.

Quaicoe, E.H., C.M. Amoah and G.T. Obodai.2014. Nutrient requirements and environmental conditions for the cultivation of the medicinal mushroom (Lentinula edodes) (Berk.) In Ghana Odamtten International Journal of Scientific and Technology Research 3: 76-84.

Royse, D.J. and J.E. Sanchez-Vazquez. 2001. Influence of substrate wood-chip particle sizeon shiitake (Lentinula edodes) yield. Bioresource Technology 76: 229-233.

Zagrean, V., G. Neata and B. Stanciulescu.2017. Influence of temperature on Mycelial growth of some Pleurotus eryngii and Lentinula edodes strains in vitro. Bulletin UASVM Horticulture 74(1): 1843-5254.

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Submitted

2021-09-29

Published

2021-10-11

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Articles

How to Cite

In vitro evaluation for optimum growth conditions of shiitake mushroom (Lentinula edodes). (2021). Mushroom Research, 29(2). https://epubs.icar.org.in/index.php/MR/article/view/116196