Morphological variability in Verticillium fungicola isolates collected from different mushroom farm of Haryana state

Abstract

In present investigation, the morphological variability were studied by collecting seventeen isolatesof V. fungicola from different mushroom farms of Haryana state and out of these eight were isolated, purified on PDA medium and which coded as MHS (Hisar), BFT (Fatehabad), NJN (Jind), RHT (Rohtak),TPN (Panipat), BSN (Sonipat), FDB (Fridabad) and SKK (Kurukshetra) and pathogenicity was proved on host A. bisporus. Regarding morphological variability, the radial growth was fastest in isolate BSN (44.66 mm), followed by TPN (43.86 mm), FDB (43.33 mm), SKK (42.16 mm) and RHT (41.50mm), while isolates MHS, BFT and NJN had slow growth i.e. 35.83, 34.50 and 38.00 mm, respectively after 12 days of incubation. Isolates MHS, BFT and NJN showed uneven and less feathery colony growth, while rest of isolates had even, profuse feathery and raised colony. Colony pigmentation of V. fungicola isolates was white and underside light yellow in MHS, BFT, NJN and RHT, whereas, isolates TPN, BSN, FDB and SKK having dark yellow colour. The size of conidia also varied among the isolates and it ranges from 2.4-5.1×1.2-2.1μm (BFT) to 3.0-7.9×1.1-2.5μm (BSN) whereas, isolates MHS (2.8-4.1×1.0-2.1 μm), BFT (2.4-5.1×1.2-2.1μm) and NJN (2.6-3.1×1.0-2.4 μm) having small size conidia and rest one i.e. RHT (2.9-5.5×1.0-2.1μm), TPN (2.9-6.5×1.1-2.3 μm), BSN (3.0-7.9×1.1-2.5 μm), FDB(3.0-6.5×1.0-2.2 μm) and SKK (2.8-4.1×1.0-2.1 μm) had large sized conidia.