Evaluation of different sawdust substrates for spawn production of shiitake mushroom [Lentinula edodes (Berk.)]


Abstract views: 116 / PDF downloads: 67

Authors

  • - Tarushi Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur. 176061, (HP), India
  • Deepika Sud Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur. 176061, (HP), India
  • Arun Sud Directorate of Extension Education, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur. 176061, (HP), India

Abstract

Shiitake mushroom is one of the most widely cultivated specialty mushrooms in the world. It is considered as one of the delicious, edible mushrooms which has medicinal properties. Quality spawn production is the key factor in its successful cultivation. More vigorous the spawn, better will be the fruit production. Sawdust is the most popular basic ingredient used for producing spawn for shiitake. However, there is need to evaluate the best sawdust substrate for synthetic log cultivation of shiitake mushroom. In the present study, five locally available sawdust substrates of broad-leaved trees, viz., eucalyptus, mango, mulberry, poplar and tooni were evaluated to produce shiitake spawn following standard procedure. The growth of the spawn was measured in terms of MRR [Mycelial Run Rate (cmday-1)]. Observations revealed that the MRR on eucalyptus substrate formulation was fastest amongall other formulations. Shiitake mushroom required 44 days to complete mycelial growth on eucalyptus followed by 54 days on tooni while all other substrates took more than 60 days for complete growth. The MRR on different substrates ranged from 0.16 to 0.30 cm day-1. The highest MRR was observed in eucalyptus sawdust (0.30 cm day-1) followed by tooni (0.25 cm day-1). There was no significant difference in the MRR of mulberry and poplar when used as sawdust substrates. The lowest MRR was recorded on mango sawdust i.e. 0.16 cm day-1. The higher rate of mycelial running in eucalyptus sawdust may be due to the presence of right proportion of alpha-cellulose, hemi-cellulose, pectin, lignin and suitable C: N ratio.

Downloads

Download data is not yet available.

References

Annepu, S.K., V.P. Sharma, S. Kumar, A.Barh, S. Banyal and S. Kamal. 2018. Enzyme profile of shiitake mushroom strains grown on wheat straw. Indian Journal of Horticulture 75(3): 475-481.

Annepu, S.K., V.P. Sharma, A. Barh, S.Kumar, M. Shirur and S. Kamal. 2019. Effects of genotype and growing substrate on bioefficiency of gourmet and medicinal mushroom, Lentinula edodes (Berk.) Pegler. Bangladesh Journal of Botany 48(1): 129-138.

Ashrafuzzaman, M., A.K.M. Kamruzzaman, M.R. Ismail, S.M. Shahidullah and S.A. Fakir.2009a. Substrate affects the growth and yield of shiitake mushroom. African Journal of Biotechnology 8(13): 2999-3006.

Ashrafuzzaman, M., A.K.M. Kamruzzaman,M.R. Ismail and S.M. Shahidullah. 2009b.Comparative studies on the growth and yield of shiitake mushroom (L.edodes) on different substrates. Advances in Environmental Biology 3(2): 195-203.

Bisen, R.K. B.S. Baghel, G.S. Sanodiya and G.B.K.S Prasad. 2010. Lentinus edodes: A Macrofungus with Pharmacological Activities. Current Medicinal Chemistry 17: 2419-2430.

Chang, S.T. and P.G. Miles. 2004. Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact, 2nd ed., CRC Press, Boca Raton, FL P.S. 5.

Delpech, P. and J.M. Olivier. 1991. Cultivation of shiitake on straw based pasteurized substrates. In: Maher, J. (ed) Science and Cultivation of Edible Fungi. Balkema, Rotterdam, pp 523-528.

Donoghue, J.D., A.K. Somonson and W.C. Denison. 1996. Spawning techniques for sawdust based shiitake production: past, present and future. Mushroom News 44(7): 6-17.

Elisashvili, V., E. Kachlishvili and M. Asatiani.2015. Shiitake Medicinal Mushroom, Lentinus edodes (Higher Basidiomycetes) Productivity and Lignocellulolytic Enzyme Profiles during Wheat Straw and Tree Leaf Bioconversion. International Journal of Medicinal Mushrooms 17(1): 77-86.

Grodzinskaya, A.A., H.D. Infante and N.M.Piven. 2003. Cultivation of edible mushrooms using agricultural and industrial wastes. Agronomia Tropical (Maracay) 52: 427-447.

Hobbs, Ch. 1995. Medicinal Mushrooms: An Exploration of Tradition, Healing, and Culture,2nd Ed.; Botanica Press, Inc.: Santa Cruz, CA,USA.

Ivan, H.R., A.C. Monteiro, J.O. Machado andJ.L. Andrioli. 2003. Shiitake (Lentinulaedodes) production on a sterilized bagasse substrate enriched with rice bran and sugarcane molasses. Brazilian Journal of microbiology34(1): https://doi.org/10.1590/S1517-83822003000100014

Joshi, M. and A. Sagar. 2016. Evaluation of various substrates for spawn production and cultivation of shiitake mushroom using corncobs. Mushroom Research 25(2): 119-124.

Kaur, M.J. and T.N. Lakhanpal. 1995.Cultivation of Japanese mushroom shiitake (L.edodes) in India. Indian J Microbiol 35:339-342

Kozak, M.E. and J. Krawczyk. 1989. Growing Shiitake Mushrooms in a Continental Climate.ABC Printers, Marinette.

Mata, G., P. Delpech and J.M. Savoie. 2001. Selection of strains of Lentinula edodes and Lentinula boryana adapted for efficient mycelial growth on wheat straw. Revista Iberoamericana de Micología 18: 118-122.

Miller, M.W. and S.C. Jong. 1987. Commercial cultivation of shiitake in sawdust filled plastic bags. Dev Crop Sci 10: 421-426.

Palomo, A., C. Door and L. Mattos. 1998.Comparative study of different substrates for the growth and production of Lentinus edodes Berk (“Shiitakeâ€). Fitopatologia 33: 71-75.

Przybylowicz, P. and J. Donoghue. 1988. Shiitake Growers Handbook: the Art and Science of Mushroom Cultivation. Dubuque, Iowa: Kendall/Hunt Publishing Company.

Puri, S. 2011. Agricultural wastes as substratefor spawn production and their effect on shiitakemushroom cultivation. International Journal of Science and Nature 2(4): 733-736.

Savoie, J.M., P. Delpech, C. Billete and G.Mata. 2000. Inoculum adaptation changes theoutcome of competition between Lentinula edodes and Trichoderma spp. during shiitake cultivation on pasteurized wheat straw. Mush Sci 15: 667-674.

Sharma, V.P. and S.K. Annepu. 2018.Advancement in medicinal mushroom research. In: Singh B., Peter K. (eds) New Age Herbals. Springer, Singapore. https://doi.org/10.1007/978-981-10-8291-7-8.

Sharma, S.R., S. Kumar and V.P. Sharma.2006. Physiological requirement for cultivation of Malaysian strain of shiitake mushroom. Journal of Mycology and Plant Pathology36: 149-152.

Sharma, V.P., S. Kumar and S.R. Sharma.2011. Cultivation of shiitake (Lentinulaedodes). In: Mushrooms Cultivation, Marketing and Consumption (Singh, M., B. Vijay, S.Kamal and G.C. Wakchaure eds.) p. 207-14, Directorate of Mushroom Research (ICAR),Solan, (HP), India.

Shukla, A.N. 1995. Effect of hormones on the production of shiitake, Lentinus edodes (Berk.)Sing. Mushroom Res 4: 39-42.

Singh,Y. and H.S. Sidhu. 2014. Management of cereal crop residues for sustainable rice-wheat production system in the Indo-Gangetic Plains of India. Proceedings of the Indian Academy of Sciences. 80(1): 95-114.

Downloads

Submitted

2021-09-30

Published

2021-10-11

How to Cite

Tarushi, .-., Sud, D., & Sud, A. (2021). Evaluation of different sawdust substrates for spawn production of shiitake mushroom [Lentinula edodes (Berk.)]. Mushroom Research, 29(2). Retrieved from https://epubs.icar.org.in/index.php/MR/article/view/116258

Issue

Section

Articles