Molecular characterization of bacterial isolates causing brown blotch of cultivated mushrooms


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Authors

  • V P Sharma Directorate of Mushroom Research, Solan –173213
  • Satish Kumar Directorate of Mushroom Research, Solan –173213
  • Vanita Gautam Directorate of Mushroom Research, Solan –173213

Abstract

The polymerase chain reaction was used to amplify 16S ribosomal DNA from blotch causing bacteria isolated from five cultivated mushrooms. The bacteria were identified as Pseudomonas tolaasii and P. reactans. PCR-  amplified 16S rDNA was analyzed on the basis of nucleotide sequence . Genetic diversity among the isolates was studied using arbitrary RAPD primers. All the fourteen isolates were categorized into five groups showing 53 per cent similarity.

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Submitted

2015-05-28

Published

2010-12-31

Issue

Vol. 19 No. 2 (2010)

Section

Articles

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I/We hereby assign copyright to the Mushroom Research Journal for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re-use of matter). 

How to Cite

Sharma, V. P., Kumar, S., & Gautam, V. (2010). Molecular characterization of bacterial isolates causing brown blotch of cultivated mushrooms. Mushroom Research, 19(2). https://epubs.icar.org.in/index.php/MR/article/view/48892