Separation and determination of antimicrobial activities of targeted ganoderic acids from Ganoderma lucidum (Lingzhi)

Abstract

Reversed phase high performance liquid chromatography (HPLC) was performed for separation of targeted ganoderic acids of Ganoderma lucidum used in herbal formulations for control of various diseases. The samples were collected from various agro-climatic zones of Haryana. The methanol mobile phase for isocratic elution  was used and  the sample showed two single compound peaks which were subsequently identified as ganoderic acidT (GA-T) and ganoderic acid – Me (GA-Me) respectively, based on their retention  time using diode array UV peak purity scans at ^max 245 nm. The retention time for GA-T was 3 min and for GA-ME was 7 minutes. These ganoderic acids were subjected for antibacterial and antifungal activities against indigenous populations of Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus flavus, Mucor and Populospora byssina. The minimum inhibitory counteraction was found to be 150 µg/ml for bacterial cultures with maximum inhibitory days varying between 10-14 days. The same was 100 µg/ml and 18-31 days, respectively, for fungal cultures. The study indicates that the concentration of triterpenoids of G lucidum is necessary for quality control when it is used as herbal medicine.