Mycelial biomass under submerged culture conditions and antioxidant activity of two wild edible mushrooms of Tripura
229 / 54
Authors
-
Aparajita Roy Das
Tripura University
-
Ajay Krishna Saha
Tripura University
-
RC Upadhyay
Directorate of Mushroom Research
-
Panna Das
Tripura University
Abstract
The optimization of mycelial growth of two edible mushroom using different carbon and nitrogen source under submerged culture condition was tested using basal synthetic media replacing the various carbon and nitrogen source. In addition, antioxidant potential was determined using peroxidase, superoxide dismutase and catalase activity. The highest dry weight was noted in Lentinus tuber-regium and Macrocybe gigantea using dextrose as a carbon source. The best nitrogen source found in L. tuber-regium and M. gigantea was peptone and yeast extract, respectively. The growth of mycelial biomass is slow and less in M. gigantea using carbon source; however, addition of nitrogen source increases the biomass yield. M. gigantea exhibited the highest peroxidase activity, superoxide dismutase activity and catalase activity. The best source of carbon and nitrogen can be used for maximum biomass yield of two studied mushroom. Moreover, the study reveals both the species of mushroom have a rich source of antioxidant property.
References
Agrahar-Murugkar, D. and G. Subbulakshmi. 2005. Nutritional value of edible wild mushrooms collected from the Khasi hills Meghalaya. Food Chemistry 89:599-603.
Ajith, T.A. and K.K. Janardhanan. 2001. Antioxidant and antiinfammatory activities of methanol extract of Phellinus rimosus. Indian J. Exp. Biol. 39:1166-1169.
Anyakorah, C.I., N. Okafo and O. Olatunji. 2004. Carbon And Nitrogen Requirements For The Cultivation Of Oyster Mushroom Pleurotus sajor-caju. Nigerian Food Journal 22 (1): 127-132
Barros, L., M.J. Ferreira, B. Queiros, I.C.F.R. Ferreira and P. Baptista. 2007. Total phenols, ascorbic acid, â-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chemistry 103: 314–419.
Burns, P.J., P. Yeo, T. Keshavarz, S. Roller and C.S. Evans. 1994. Physiological studies of exopolysaccharide production from the basidiomycete Pleurotus sp. florida. Enzyme Microbial Technology 16: 566–572.
Das, A.R., M. Borthakur, A.K. Saha, S.R. Joshi, and P. Das. 2015. Growth of mycelial biomass and fruit body cultivation of Lentinus squarrosulus collected from home garden of Tripura in Northeast India. J App Biol Biotech 3(04):017-019.
Elmastas, M., O. Isildaka, I. Turkekulb and N. Temura. 2007. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Composition and Analysis 20: 337–345.
Gu, F.H., H. Yin and J. Ma. 2001. The influences of different carbon and nitrogen sources on mycelial growth and extracellular polysaccharide yields of Polyporus umbellatus. Journal of Northwest University (Natural Science Edition) 31: 437-440.
Kim, H.O., J.M. Lim, J.H. Joo, S.W. Kim, H.J. Hwang, et al. 2005. Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea. Bioresource Technology 96: 1175–1182.
Lee, I.K., Y.S. Kim, Y.W. Jang, J.Y. Jung and B.S. Yun. 2007. New antioxidant polyphenols from the medicinal mushroom Inonotus obliquus. Bioorg.Med. Chem. Lett. 17: 6678-6681.
Li, T.Y., G.Y. Tian and G.B. Xu. 2007. Effect of polysaccharides from mycelial of Grifula umbellateon mice’s immunological level. Chin. J. Vet. Surg. 27: 88-94.
Lindequist, U., T.H.J. Niedermeyer and W.D. Julich. 2005. The pharmacological potential of mushrooms. eCAM. 2(3): 285-299.
Liu, R.S., D.S. Li, H.M. Li and Y.J. Tang. 2008. Response surface modeling the significance of nitrogen source on the cell growth and Tuber polysaccharides production by submerged cultivation of Chinese truffle Tuber sinense. Process Biochem 43: 868-876.
Marklund, S. and G. Marklund. 1974. Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J.Biochem. 47: 469-474.
Niki, E., H. Shimaski and M. Mino. 1994. Antioxidantism – free radical and biological defence. Tokyo: Gakkai Syuppan Center.
Pokhrel, C.P. and S. Ohga. 2007. Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes. Food Chemistry 105: 641-646.
Ramesh, V., C. Karunakaran and A. Rajendran. 2014. Optimization of submerged culture conditions for mycelial biomass production with enhanced antibacterial activity of the medicinal macro fungus Xylaria sp. Strain R006 against drug resistant bacterial pathogens. Current Research in Environmental and Applied Mycology 4 (1): 88–98.
Sadasivam, S. and A. Manickam. 2004. In: Biochemical Methods, (Eds. Sadasivam, S. and Manickam, A.), New Age International (P) Ltd. Publishers, Coimbatore, India
Saha, A.K., S. Acharya and A. Roy. 2012. Antioxidant level of wild edible mushroom: Pleurotus djamor (Fr.) Boedijn. Journal of Agricultural Technology 8(4): 1343-1351.
Shih, I.L., K. Pan and C. Hsieh. 2006. Influence of nutritional components and oxygen supply on the mycelial growth and bioactive metabolites production in submerged culture of Antrodia cinnamomea. Process Biochemistry 41: 1129–1135.
Song, C.H. and K.Y. Cho. 1987. A synthetic medium for the production of submerged cultures of Lentinus edodes. Mycologia 79: 866–876.
Sun, C., C.Y. Shan, X.D. Gao and R.X. Tan. 2005. Protection of PC12 cells from hydrogen peroxide-induced injury by EPS2, an exopolysaccharide from a marine filamentous fungus Keissleriella sp. YS4108. J. Biotechnol. 115: 137-144.
Sung, G.H., B. Shrestha and J.M. Sung. 2010. Characteristics of Metacordyceps yongmunensis, a New Species from Korea. Mycobiology 38(3): 171-175.
Wang, L.L., Wu, H.Y. and Luo, D.F. 2000. The pharmacological effects and clinical application of Polyporus umbellatus. China Pharmaceut 9: 58-59.
Wani, B.A., R.H. Bodha and A.H. Wani. 2010. Nutritional and medicinal importance of mushrooms. J. Med. Plants Res. 4(24): 2598-2604.
Wasser, S.P. and A.L. Weis. 1999. Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: Current perspectives (Review). Int J Med Mushrooms 1: 31–62
Xiao, J.H., D.X. Chen, W.H. Wan, X.J. Hu and Y. Qi, et al. 2006. Enhanced simultaneous production of mycelia and intracellular polysaccharide in submerged cultivation of Cordyceps jiangxiensis using desirability functions. Process Biochemistry 41: 1887-1893.
Yang, F.C., H.C. Huang and M.J. Yang. 2003. The influence of environmental conditions on the mycelial growth of Antrodia cinnamomea in submerged cultures. Enzyme and Microbial Technology 33: 395-402.
Zhou, X.Y., S.M. Gu, W.Y. Zhang and Z.S. Xu. 2001. Fermentative production of mycelia by Polyporus umbellatus (Pers) Frie. Ind. Microbiol. 31: 1-4.
Downloads
Submitted
Published
Issue
Section
License
I/We have taken permission from the copyright holder to reproduce the matter not owned by me and acknowledged the source.
I/We permit editors to publish the said paper in the journal or in any other means with editorial modification, if any.
In case of publication of the article in the journal,
I/We hereby assign copyright to the Mushroom Research Journal for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re-use of matter).Â