Fatty acid, Amino acid, Bioactive compounds and Anti-microbial properties of wild Volvariella volvaceae from Kokrajhar, Assam, INDIA.


289

Authors

  • RAJU ALI Department of Biotechnology Bodoland University
  • MAMONI RAVA Department of Biotechnology, Bodoland University
  • SANDEEP DAS Department of Biotechnology

Abstract

Abstract:  Volvariella volvaceae were collected from selected location of Kokrajhar followed by subsequent culture in  PDA media. Mushrooms were oven dried and extract was prepared using solvent (chloroform: methanol: water) for fatty acid analysis by GC-MS.  Total of 22 fatty acid compounds were detected with higher content of unsaturated fatty acids. Linoleic acid was dominant among all.  Amino acid analysis was done by digesting the samples with 6N HCl then estimated by UHPLC. A total of 21 amino acids were detected using 42 amino acids as  standards, the samples have both standard and non standard amino acids, hydroxyl proline was found to be highest in concentration with 8.66 mg/100 gm for non standard, threonine with 2.2 mg for standard amino acids. The bioactive compounds were screened from methanolic extract by GC-MS analysis and it was found that the sample was rich in many bioactive compounds including sterols, quinols, alkanes and fatty acids. The antimicrobial screening of samples were carried out against 5 microbial cultures acquired from IMTECH and it was found to inhibit the growth of microbes by ethanolic, methanolic, aqueous and petroleum ether extracts to significant extent.

Author Biographies

  • RAJU ALI, Department of Biotechnology Bodoland University
    Rsearech Scholar, Department of Biotechnology, Bodoland University
  • MAMONI RAVA, Department of Biotechnology, Bodoland University
    Research scholar, Department of Biotechnology, Bodoland University
  • SANDEEP DAS, Department of Biotechnology

    Associate Professor & Head

    Department of Biotechnology

    Bodoland University

References

Agoramoorthy, G., Chandrasekaran, M., Venkatesalu, V., & Hsu, M. J. (2007). Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Brazilian Journal of Microbiology, 38(4), 739-742.

Ali, R., Rava, M., Boro, H., Das, S. 2017. Studies on growth, anti-oxidant properties, minerals, macro and micro-morphological characters of Volvariella volvaceae collected from Kokrajhar, (Assam), India. Mushroom Research 26 (2) : 165-174,.

.3. Aoki, M., Suto, K., Komatsu, M., Kamimura, A., Morishita, K., Yamasaki, M., & Takao, T. (2012). Increasing effect of an oral intake of L-hydroxyproline on the soluble collagen content of skin and collagen fragments in rat serum. Bioscience, biotechnology, and biochemistry, 76(6), 1242-1244.

Ayodele, S. M., & Idoko, M. E. (2011). Antimicrobial activities of four wild edible mushrooms in Nigeria. Internatinaol Journl of Science and Nature, 2(1), 55-58.

Barros, L., Baptista, P., Correia, D. M., Casal, S., Oliveira, B., & Ferreira, I. C. (2007). Fatty acid and sugar compositions, and nutritional value of five wild edible mushrooms from Northeast Portugal. Food Chemistry, 105(1), 140-145.

Baker, D. H. (2009). Advances in protein–amino acid nutrition of poultry. Amino acids, 37(1), 29-41.

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79.

Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian journal of biochemistry and physiology, 37(8), 911-917.

Brasseâ€Lagnel, C., Lavoinne, A., & Husson, A. (2009). Control of mammalian gene expression by amino acids, especially glutamine. The FEBS journal, 276(7), 1826-1844.

Bruhat, A., Chérasse, Y., Chaveroux, C., Maurin, A. C., Jousse, C., & Fafournoux, P. (2009). Amino acids as regulators of gene expression in mammals: molecular mechanisms. Biofactors, 35(3), 249-257.

CFR Ferreira, I., A Vaz, J., Vasconcelos, M. H., & Martins, A. (2010). Compounds from wild mushrooms with antitumor potential. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 10(5), 424-436.

Das, U. N. (2006). Essential fatty acids: biochemistry, physiology and pathology. Biotechnology Journal: Healthcare Nutrition Technology, 1(4), 420-439.

Günç Ergönül, P., Akata, I., Kalyoncu, F., & Ergönül, B. (2013). Fatty acid compositions of six wild edible mushroom species. The Scientific World Journal, 2013.

Huang, B. H., Yung, K. H., & Chang, S. T. (1989). Fatty acid composition of Volvariella volvacea and other edible mushrooms. Mushroom Science, 12, 533-540.

KalaÄ, P. (2009). Chemical composition and nutritional value of European species of wild growing mushrooms: A review. Food chemistry, 113(1), 9-16.

Kim, S. W., McPherson, R. L., & Wu, G. (2004). Dietary arginine supplementation enhances the growth of milk-fed young pigs. The Journal of Nutrition, 134(3), 625-630.

Lee, K. J., Yun, I. J., Kim, K. H., Lim, S. H., Ham, H. J., Eum, W. S., & Joo, J. H. (2011). Amino acid and fatty acid compositions of Agrocybe chaxingu, an edible mushroom. Journal of Food Composition and Analysis, 24(2), 175-178.

Lin, S. Y., Chen, Y. K., Yu, H. T., Barseghyan, G. S., Asatiani, M. D., Wasser, S. P., & Mau, J. L. (2013). Comparative study of contents of several bioactive components in fruiting bodies and mycelia of culinary-medicinal mushrooms. International journal of medicinal mushrooms, 15(3).

Mateo, R. D., Wu, G., Bazer, F. W., Park, J. C., Shinzato, I., & Kim, S. W. (2007). Dietary L-arginine supplementation enhances the reproductive performance of gilts. The Journal of Nutrition, 137(3), 652-656.

McGaw, L. J., Jäger, A. K., & Van Staden, J. (2002). Isolation of antibacterial fatty acids from Schotia brachypetala. Fitoterapia, 73(5), 431-433.

Mizuno, T. (1995). Bioactive biomolecules of mushrooms: food function and medicinal effect of mushroom fungi. Food Reviews International, 11(1), 5-21.

Pai, S. H., Jong, S. C., & Lo, D. W. (1990). Usages of mushrooms. Bioindustry, 1, 126-131.

Perera, W. A. S. W., Abeytunga, D. T. U., & Wijesundara, R. L. C. (2001). Antibacterial activities of Volvariella volvacea.

Poucheret, P., Fons, F., & Rapior, S. (2006). Biological and pharmacological activity of higher fungi: 20-year retrospective analysis. Cryptogamie Mycologie, 27(4), 311.

Rahi, D. K., & Malik, D. (2016). Diversity of mushrooms and their metabolites of nutraceutical and therapeutic significance. Journal of Mycology, 2016.

Ruess, L., Häggblom, M. M., Zapata, E. J. G., & Dighton, J. (2002). Fatty acids of fungi and nematodes—possible biomarkers in the soil food chain?. Soil Biology and Biochemistry, 34(6), 745-756.

Sadler, M. (2003). Nutritional properties of edible fungi. Nutrition Bulletin, 28(3), 305-308

Sales-Campos, H., Reis de Souza, P., Crema Peghini, B., Santana da Silva, J., & Ribeiro Cardoso, C. (2013). An overview of the modulatory effects of oleic acid in health and disease. Mini reviews in medicinal chemistry, 13(2), 201-210.

Seidel, V., & Taylor, P. W. (2004). In vitro activity of extracts and constituents of Pelagonium against rapidly growing mycobacteria. International journal of antimicrobial agents, 23(6), 613-619.

Senatore, F. (1990). Fatty acid and free amino acid content of some mushrooms. Journal of the Science of Food and Agriculture, 51(1), 91-96.

Senatore, F., Dini, A., Marino, A., & Schettino, O. (1988). Chemical constituents of some basidiomycetes. Journal of the Science of Food and Agriculture, 45(4), 337-345.

Shizuka, F., Kido, Y., Nakazawa, T., Kitajima, H., Aizawa, C., Kayamura, H., & Ichijo, N. (2004). Antihypertensive effect of γâ€amino butyric acid enriched soy products in spontaneously hypertensive rats. Biofactors, 22(1â€4), 165-167.

Singal, S. A., Hazan, S. J., Sydenstricker, V. P., & Littlejohn, J. M. (1953). The effect of threonine deficiency on the synthesis of some phosphorus fractions in the rat. Journal of Biological Chemistry, 200(2), 875-882.

Stamets, P. (2000). Growth parameters for gourmet and medicinal mushroom species, growing gourmet and medicinal mushrooms.

Tsuji, K., Ichikawa, T., Tanabe, N., Abe, S., Tarui, S., & Nakagawa, Y. (1992). Effects of two kinds of koji on blood pressure in spontaneously hypertensive rats. Journal of the Agricultural Chemical Society of Japan (Japan).

Wang, J., Chen, L., Li, P., Li, X., Zhou, H., Wang, F., ... & Wu, G. (2008). Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. The Journal of nutrition, 138(6), 1025-1032

Wasser, S. P. (2002). Review of medicinal mushrooms advances: good news from old allies. HerbalGram.

Wasser, S. P., & Weis, A. L. (1999). Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives. International Journal of medicinal mushrooms, 1(1).

Wasser, S. P. (1995). New and noteworthy species of the genus Agaricus L.: Fr. emend. Karst. from Israel. Documents Mycologiques, 25, 98-100.

Woldegiorgis, A. Z., Abate, D., Haki, G. D., Ziegler, G. R., & Harvatine, K. J. (2015). Fatty acid profile of wild and cultivated edible mushrooms collected from Ethiopia. Journal of Nutrition & Food Sciences, 5(3),

Wu, G. (2009). Amino acids: metabolism, functions, and nutrition. Amino acids, 37(1), 1-17.

Wu, G., Bazer, F. W., Davis, T. A., Kim, S. W., Li, P., Rhoads, J. M., ... & Yin, Y. (2009). Arginine metabolism and nutrition in growth, health and disease. Amino acids, 37(1), 153-168.

Wu, G., Bazer, F. W., Burghardt, R. C., Johnson, G. A., Kim, S. W., Knabe, D. A., ... & Spencer, T. E. (2011). Proline and hydroxyproline metabolism: implications for animal and human nutrition. Amino acids, 40(4), 1053-1063.

Wu, X., Ruan, Z., Gao, Y., Yin, Y., Zhou, X., Wang, L., ... & Wu, G. (2010). Dietary supplementation with L-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn-and soybean meal-based diet. Amino acids, 39(3), 831-839.

Wu, G., Knabe, D. A., & Kim, S. W. (2004). Arginine nutrition in neonatal pigs. The Journal of Nutrition, 134(10), 2783S-2790S.

Wu, G. (2010). Functional amino acids in growth, reproduction, and health. Advances in Nutrition, 1(1), 31-37.

Yao, K., Yin, Y. L., Chu, W., Liu, Z., Deng, D., Li, T., ... & Wu, G. (2008). Dietary arginine supplementation increases mTOR signaling activity in skeletal muscle of neonatal pigs. The Journal of nutrition, 138(5), 867-872.

Yoshida, A., Ashida, K., & Harper, A. E. (1961). Prevention of fatty liver due to threonine deficiency by moderate caloric restriction. Nature, 189(4768), 917-918.

Zeng, X., Wang, F., Fan, X., Yang, W., Zhou, B., Li, P., ... & Wang, J. (2008). Dietary arginine supplementation during early pregnancy enhances embryonic survival in rats. The Journal of nutrition, 138(8), 1421-1425.

Submitted

2018-09-18

Published

2019-03-24

Issue

Section

Articles

How to Cite

ALI, R., RAVA, M., & DAS, S. (2019). Fatty acid, Amino acid, Bioactive compounds and Anti-microbial properties of wild Volvariella volvaceae from Kokrajhar, Assam, INDIA. Mushroom Research, 27(2). https://epubs.icar.org.in/index.php/MR/article/view/83279