Antibacterial Property of Protein and Lipid from Pleurotus ostreatus against Ornithobacterium sp infection on poultry
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Authors
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VAJIHA BANU H
JAMAL MOHAMED COLLEGE
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RAJA A
Jamal Mohamed College(Autonomous)
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GAJALAKSHMI P
DHANALAKSHMI SRINIVASAN COLLEGE OF ARTS AND SCIENCE FOR WOMEN
Abstract
The genus Ornithobacterium is a gram-negative, sporadic bacterium in poultry, accounting millions of dollars losses to the poultry industry annually. The objective of this study is to develop a natural antibacterial biomolecules from Pleurotus ostreatus against poultry associated pathogens. Antimicrobial activity of protein and lipid isolated from Pleurotus ostreatus was evaluated against Ornithobacterium sp causes respiratory disease in poultries by stroke method. Test pathogen was isolated from infected chicks and identified by biochemical studies. Bio molecule protein isolated from Pleurotus ostreatus filtrate showed 38 mm zone of inhibitiony and its Minimum Inhibitory (MIC) concentration was found to be 8 µg / ml. protein band eluted from SDS PAGE with 1,500 Da showed positive antibacterial result against Ornithobacterium sp. Similarly isolated lipid also showed good antibacterial activity against the test pathogen with 16 µg / ml of MIC. The study describes an important step towards the development of a new drug from edible mushroom source as supplement and its significant results were observed. Further studies on purification and in vivo studies are needed to find out the efficacy of biomoleculesReferences
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