ENZYME ESTIMATION BASED ON BACTERIAL INTERACTIONs WITH Pleurotus SPP.
273 / 61
Abstract
Lignocellulosic material is the most abundant renewable source composed of lignin, hemicelluloses and cellulose present on earth. Lignin acts as a physical barrier to underneath cellulose which can be used for the production of useful products. Some white rot fungi such as Pleurotus spp. has the capability of degradation due to the production of a plethora of ligninolytic enzymes. This degradation process can be improved by coculturing the Pleurotus spp. with lignin degrading bacterial spp. In this study, the coculturing of two bacterial spp. Bacillus and Delftia with Pleurotus spp. i.e. Pleurotus florida and Pleurotus sajor-caju was investigated. No antagonistic effect was observed in the interaction of the bacterial strains with fungal species. During ligninolytic enzyme production studies on Mushroom Minimal Media (MMM) containing paddy straw as an inducer, it was observed that enzyme production was more in media inoculated with bacterial species. Therefore, coculturing of the bacteria with Pleurotus can enhance the digestibility of the paddy straw.
References
Ahmed S A, Kadam J A, Mane V P, Patil S S and Baig M M V (2009) Biological efficiency and nutritional contents of Pleurotusflorida (Mont.) Singer cultivated on different agro-wastes. Nat Sci 7: 44-48.
Alvira P, Tomás-Pejó E, Ballesteros M and Negro MJ (2010) Pre-treatmenttechnologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. BioresourTechnol101:4851-61.
Anonymous (2015) Management and utilization of paddy straw in Punjab. Department of Science, Technology & Environment, Government of Punjab, pp. 1-23.
Bennett JW andWunch KG(2002) Faison B.D.Use of fungi in biodegradationC.J. Hurst (Ed.), Manual of Environmental Microbiology, AMS press, Washington DC pp. 960-971.
Brule ´ C, Frey-Klett P, Pierrat J, Courrier S, Ge ´rard F, Lemoine M, et al (2001) Survival in the soil of the ectomycorrhizal fungus Laccariabicolor and the effects of a mycorrhiza helper Pseudomonas fluorescens. Soil BiolBiochem33(12):1683-94.
Chakraborty U and Sikdar S R (2008) Production and characterization of somatic hybrids raised through protoplast fusion between edible mushroom strains Volvariellavolvacea andPleurotusflorida. World J MicrobiolBiotechnol24:1481-92.
Chiaramonti D, Prussi M, Ferrero S, Oriani L, Ottonello P, Torre P andCherchi F (2012) Review of pre-treatment processes for lignocellulosic ethanol production and development of an innovative method. Biomass Bioenerg46:25-35.
Cho Y S, Kim J S, Crowley D E and Cho B G (2003) Growth promotion of the edible fungus Pleurotusostreatus by fluorescent pseudomonads.FEMSMicrobiolLett218: 271-76.
Duponnois R, Garbaye J (1991) Mycorrhization helper bacteria associated with the Douglas fir-Laccarialaccata symbiosis: effects in aseptic and in glasshouse conditions. Ann ForSci48(3):239-51.
Ergun S O and Urek R O (2017) Production of ligninolytic enzymes by solid state fermentation using Pleurotusostreatus.Ann AgricSci15: 273-77.
Febriansyah E, Saskiawan I, Mangunwardoyo W, Sulistiyani T R and Widhiya E W (2018) Potency of growth promoting bacteria on mycelial growth of edible mushroom Pleurotusostreatus and its identification based on 16S rDNA analysis Cite as: AIP Conference Proceedings 2002, 020023; https://doi.org/10.1063/1.5050119
Frey-Klett P, Burlinson P, Deveau A, Barret M, Tarkka M, Sarniguet A (2011) Bacterial-fungal interactions: hyphens between agricultural, clinical, environmental, and food microbiologists. MicrobiolMolBiol Rev75(4):583-609.
Gadde B, Bonnet S, Menke C and Garivait S (2009) Air pollutant emissions from rice straw open field burning in India, Thailand and the Phillipines.Environ Pollut157: 1554-58.
Jurado M, Lopez M J, Suárez-Estrella F, Vargas-GarcÃa M C, Lopez-Gonzalez J A and Moreno J (2014) Exploiting composting biodiversity: study of the persistent and biotechnologically relevant microorganisms from lignocellulose-based composting. BioresourTechnol162: 283-93.
Kalyani P, Vineela K C, Geetha S and HemalathaKPJ (2016) Green Synthesis of Silver Nano Particles from Aspergillusniger (MTCC-961). Int J CurrMicrobioApplSci5(10): 50-56. ISSN: 2319-7706
Kausar H, Ismail M R, Saud H M, Habib S H, Othman R and Miah G (2016) A novel lignocellulolytic bacterium for bioconversion of rice straw. Pak J Agri Sci 53: 1-11.
Keller F, Hamilton J and Nguyen Q (2003) Microbial pre-treatment of biomass. ApplBiochemBiotechnol105: 27-41.
Kim W G, Weon H Y, Seok J S and Lee K H (2008) In vitro antagonistic characteristics of Bacilli isolates against Trichoderma spp. and three species of mushrooms. Mycobio36: 266-69.
Lowry O H, Rosebrough N J, Farr A L and Randall R J (1951) Protein measurement with folin-phenol reagent. J BiolChem193: 265-75.
Lundell T K, Mäkela M and R Hilden K (2012) Lignin-modifying enzymes in filamentous basidiomycetes: ecological, functional and phylogenetic review.J Basic Microbiol50: 1-16.
Mandels M and Sternberg D (1976) Recent advances in cellulase technology. J Ferment Technol54: 267-28.
Martin R D, Rivers J P W andCowgill U M (1976) Culturing mealworms as food for animals in captivity. Int Zoo Yearb16(1): 63-70.
Miller G L (1958) Use of dinitrosalicylic acid reagent for determination of reducing sugars.Anal Chem 31: 426-28.
Mishra B K, Pandey A K and Lata (2007) Lignocellulolytic enzyme production from submerged fermentation of paddy straw.Indian J Microbiol 47: 176-79.
Oh S-Y, Kim M, Eimes J A and Lim YW (2018) Effect of fruiting body bacteria on the growth of Tricholomamatsutake and its related molds. PLoS ONE13(2): e0190948.https://doi. org/10.1371/journal.pone.0190948
Pandey A K, Gaind S, Ali A and Nain L (2009) Effect of bioaugmentation and nitrogen supplementation on composting of paddy straw.Biodegradation 20: 293-306.
Panjabrao V, Sopanrao P S, Ahmed S A andVaseem B M M(2007) Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotussajor-caju (Fr.) Singer.J Zhejiang UnivSci8(10):745-51.
Paszczynski A, Crawford R L and Huynh V B (1988) Manganese peroxidase of Phanerochaetechrysosporiumpurification.Methods Enzymol161: 264-70.
Pérez J, Muñoz-Dorado J, De-la-Rubia T andMartÃnez J (2002) Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview.IntMicrobiol5: 53-63.
Rainey P B (1991) Effect of Pseudomonas putida on hyphal growth of Agaricusbisporus. Mycol Res 95: 699-704.
Sandhu D K and Kalra M K (1982) Production of cellulases, xylanases and pectinases by Trichodermalongibrachiatum on different substrates.Trans British MycolSoc79: 409-13.
Schrey SD, Schellhammer M, Ecke M, Hampp R andTarkka MT (2005)Mycorrhiza helper bacterium Streptomyces AcH 505 induces differential gene expression in the ectomycorrhizal fungus Amanita muscaria. New Phytol168(1):205-16.
Tan Y H and Wahab M N (1997) Extracellular enzyme production during anamorphic growth in the edible mushroom, Pleurotussajor-caju.World J MicrobiolBiotechnol13: 613-17.
Tien M and Kirk T K (1983) Lignin degrading enzyme from Phanerochaetechrysosporium: Purification, characterization and catalytic properties of unique H2O2-requiring oxygenase. ProcNatlAcadSci81: 2280-84.
Toyama N and Ogawa K (1977) Cellulase production of Trichodermaviridein solid and submerged culture methods. In: Ghosh T K (Ed.) ProcSymp on Bioconversion Cellulosic Substances into Energy, Chemical and Microbial Protein, IIT, New Delhi, pp. 305-12.
Vijaya C and Singaracharya M A (2005) Cellulolytic and lignolytic enzymes produced during solid state fermentation of paddy straw by fungi. Indian J Microbiol 45: 75-77.
Zhao H, Yu H, Yuan X, Piao R, Piao H, Wang X and Cui Z (2014) Degradation of lignocelluloses in rice straw by BMC-9, a composite microbial system.J MicrobiolBiotechnol24: 585-91.
Zheng Y, Zhao J, Xu F and Li Y (2014) Pre-treatment of lignocellulosic biomass for enhanced biogad production.ProgEnerg Combust Sci42:35-53.
Downloads
Submitted
Published
Issue
Section
License
I/We have taken permission from the copyright holder to reproduce the matter not owned by me and acknowledged the source.
I/We permit editors to publish the said paper in the journal or in any other means with editorial modification, if any.
In case of publication of the article in the journal,
I/We hereby assign copyright to the Mushroom Research Journal for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re-use of matter).Â