AN EFFICIENT AGROBACTERIUM-MEDIATED TRANSFORMATION METHOD FOR POTATO C.V. KUFRI CHANDRAMUKHI
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Authors
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Anupama Singh
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Ankita Sharma
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Hemant Balasahib Kardile
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Vinay Bhardwaj
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Som
ICAR - Central Potato Research Institute
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Brajesh Singh
Keywords:
Potato, Agrobacterium tumefaciens, Transformation, RegenerationAbstract
An optimized regeneration and Agrobacterium-mediated transformation protocol based on internode explants
was developed in potato cultivar ‘Kufri Chandramukhi’. Potato internodes were transformed by cocultivation with A.
tumefaciens strain EHA 105 harboring vector pRI101. MS medium with IAA 0.042mg L-1 + GA3 3.0 mg L-1 + Zeatin 3.0 mg
L-1 showed the maximum percentage of callus formation i.e. 76% with average number of shoots per explants was 7.00.
This medium showed mimimal number of days for callus initiation as compared to other medium compositions The best
combination for shoot regeneration was a medium of Murashige & Skoog salts with 0.042 mg L-1 IAA, 3.0 mg L-1 GA3, 3.0
mg L-1 Zeatin and 0.008 mg L-1 NAA. Use of 50 mg L-1 Kanamycin, 250 mg L-1 Carbenicillin and 100 mg L-1 Cefotaxime in
the callus induction medium minimized contamination. This method is useful for genetic transformation studies in KCM.
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References
Lecardonnel A and Sangwan-Norreel BS (1998) Agrobacterium-mediated transformation of three economically important potato cultivars using sliced internodal explants: an efficient protocol of transformation. J. Expt. Botany 49: 1589-1595.
Chakravarty B Wang-Pruski, G Flinn B Gustafson V and Regan S (2007) Genetic transformation in potato: Approaches and strategies. American Journal of Potato Research. 84: 301-311.
Dhaka M and Nailwal TK (2015) High efficiency macropropagation of potato (Solanum tuberosum L.) cv. Kufri Jyoti in Kumaun Hills. J. Plant Breed. Crop Sci 7: 203-210.
Ullah I Jadoon M Rehman A Zeb T and Khan K (2012) Effect of different GA3 concentration on in vitro propagation of potato variety Desiree. Asian J. Agric. Sci 4(2):108-109
Khatun A, Hasan MM, Bachchu MAA, Moniruzzaman M and Nasiruddin KM (2012) Agrobacterium-mediated Genetic Transformation of Potato (Solanum tuberosum L.) var. Cardinal and Hera. The Agriculturists 10 (1): 81-86
Martel and E Carcia (1992) In vitro formation of adventitious shoots on discs of potato (Solanum tuberosum L. cv. Sebago) tubers. Phyton Buenos Aires 53: 57-64
Miller PR, Amirouche L, Stuchbury T and Mathews S (1985) The use of plant growth regulators in micropropagation of slow-growing potato cultivars. Potato Res 28: 479-486
Molla MMH, Nasiruddin KM, Al-Amin M, Haque MS and Maniruzzaman (2011) Agrobacterium-mediated Transformation in Potato. Thai J. of Agri. Sci 44(2): 93-102
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497
Onamu R, Juan PL, Jaime CS, Jose LR and Joel NP (2012) In vitro regeneration and Agrobacterium-mediated transformation of potato (Solanum tuberosum L.) cultivars grown in Mexico. Plant Tissue Cult. & Biotech 22(2): 93-105
Rawat TS, Krishnaprasad BT and Anil VS (2017) Direct and Indirect Regeneration of Potato Cultivar Kufri Jyoti. J. of Biotech. and Biochem 3(4): 31-34
Saker MM Tarek AA. Moussa NZH Amany HA Abo E and Abdel-Rahman RMH. (2012) Selection of an efficient in vitro micro-propagation and regeneration system for potato (Solanum tuberosum L.) cultivar Desiree. Afr. J. Biotech 11(98): 16388-16404
Sarker RH and Mustafa BM. (2002) Regeneration and Agrobacterium-mediated genetic transformation of two indigenous potato varieties of Bangladesh. Plant Tiss. Cult 12(1): 69-77
Singh A Siddappa S Bhardwaj V Singh B Kumar D and Singh BP (2015) Expression profiling of potato cultivars with contrasting tuberization at elevated temperature using microarray analysis. Plant Physiology and Biochemistry. 97: 108– 116.
Singh A, Kaushal N, Sharma R, Bhardwaj V, Singh B, et al. (2016) Effect of Elevated Temperature on In Vitro Microtuberization of Potato Genotypeswith Different Thermotolerance Levels. Vegetos 29(3): 6-9
Tran TN and Mishra SN (2015) Effect of antibiotics on callus regeneration during transformation of IR64 rice. Biotechnology Reports 7: 143–149
Yasmin S Nasiruddin KM Begum R and Talukder SK (2003) Regeneration and establishment of potato plantlets through callus formation with BAP and NAA. Asian J. Plant Sci 2(12): 936-940
Yee S, Stevens B, Coleman S, Seabrook J and Li Q (2001) High efficiency regeneration in vitro from potato petioles from intact leaflets. Am. J. Potato Res 78: 151-157
Farhatullah ZA and Abbas JS (2007) In vitro effects of gibberellic acid on morphogenesis of potato explant. Int. J. Agric. Biol 9: 200
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