MOLECULAR CHARACTERIZATION OF POTATO PARENTAL LINES FOR ENHANCED RESISTANCE TO POTATO CYST NEMATODES
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Authors
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Salej Sood
ICAR-CPRI Shimla
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Vikas Mangal
https://orcid.org/0000-0001-9418-2340
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Rajender Singh
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Ashwani Kumar
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Bhawna Dipta
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Barkha Sharma
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Vinay Bhardwaj
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Vinod Kumar
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Ashwani Kumar Sharma
-
Brajesh Singh
Keywords:
Potato cyst nematodes, Globodera rostochiensis, Globodera pallida, PCN resistance, molecular characterization, PCR amplification, resistance genesAbstract
Potato cyst nematodes (Globodera pallida and G. rostochiensis), are major pests that significantly reduce potato yields worldwide. Developing potato varieties resistant to cyst nematodes is essential to sustain potato production worldwide especially in temperate areas. In this study, we did molecular characterization of 85 potato accessions using linked markers/genes to PCN resistance. We used three QTLs/gene-linked markers for each species: G. rostochiensis (H1, GroVI, and Grp1) and G. pallida (Gpa2, Gpa5, GpaIVSadg). The screening results revealed diverse resistance levels among the accessions based on presence and absence of the marker bands. For G. pallida, 22 accessions carried both Gpa2-1 and Gpa2-2 markers, while only three showed the presence of SPUD1636 marker associated with Gpa5. Most accessions, except one, tested positive for the Contig237 marker linked to GpaIVSadg. Likewise, for G. rostochiensis, 21 accessions had the H1 gene marker TG689, while 14 showed the presence of 57R marker. The GroVI markers U14 and X02 were found in 33 and 57 accessions, respectively, and the Grp1 marker TG432 was present in 12 accessions only. Notably, two accessions, CP4052 and CP4057, exhibited seven resistance markers, making them prime candidates for breeding programs aimed at developing durable PCN resistant potato varieties. The use of molecular markers improves the efficiency of selecting resistant plants and is more cost-effective and quicker than traditional methods. This approach helps in early identification of parental lines with multiple resistance genes, aiding in gene stacking and enhancing the overall breeding process for PCN-resistant potatoes.
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