EFFICIENT IN VITRO MICROTUBER INDUCTION IN POTATO (SOLANUM TUBEROSUM L. CV. KUFRI CHANDRAMUKHI) USING HIGH-SUCROSE MS MEDIUM UNDER DARK CONDITIONS
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Authors
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SATABDI GHOSH
ASSISTANT PROFESSOR
Keywords:
Microtuberization, Solanum tuberosum, Kufri Chandramukhi, In vitro culture, MS medium, Sucrose, Cytokinin, Potato biotechnologyAbstract
The present study aimed to standardize an efficient protocol for in vitro microtuber
induction in Solanum tuberosum L. cv. Kufri Chandramukhi. Nodal explants were cultured on
Murashige and Skoog (MS) medium supplemented with 10.0 mg l-1 benzyladenine (BA) and 80.0
g l-1 sucrose to promote microtuber development under dark conditions at 20 ± 1°C. Microtubers
were harvested after 6 weeks, and their number, average weight, and diameter were recorded.
Results demonstrated successful induction of morphologically uniform microtubers from in vitro
grown microplants. The average number of microtubers per plantlet was 2.2 ± 0.4, with an average
weight of 0.460 ± 0.042 g and a diameter of 6.24 ± 0.78 mm. This protocol offers a reproducible
and scalable system for rapid microtuber production, with potential applications in seed potato
propagation, germplasm conservation, and pre-breeding programs. The study highlights the
amenability of cv. Kufri Chandramukhi to in vitro tuberization, contributing to the advancement
of potato biotechnology and seed systems in India.
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