GENOME-WIDE DISTRIBUTION ANDCHARACTERIZATION OF HIGH TEMPERATURESHOCK TRANSCRIPTION FACTOR GENES FORDROUGHT AND HIGH TEMPERATURE STRESS INSOLANUM TUBEROSUM L.
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Authors
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Anshul Sharma Manjul
-
Amarjit Kaur Nath
-
Hemant B. Kardile
-
Pinky Raigond
-
Brajesh Singh
-
Som Dutt
ICAR-CPRI, Shimla
Keywords:
chromosome location, phylogenetic, expression analysis, potato genome, expression analysisAbstract
Abiotic stresses, mainly drought and high temperature affect potato production
greatly. Molecular mechanisms associated with high temperature and drought stresses are yet to be
deciphered fully. In many crops "high temperature shock transcription factors" (hsf) play important
role in providing tolerance to plants under these stresses however, the role of hsf family in potato
(Solanum tuberosum) in relation to abiotic stress is not fully explored yet. We performed detailed
genome-wide analysis of hsf genes in potato. Fifteen hsf genes were identified in the potato genome
which were found to be scattered on all chromosomes except chromosomes 1, 3 and 5. The structural
analysis of predicted potato hsf genes indicated presence of only one intron in potato hsf genes.
The lengths of deduced hsf proteins were found to be ranging from 55 to 536 amino acids, having
molecular weights in the range of 12.20 kDa to 60.25 kDa with theoretical pI values from 4.71 to
9.58. The grand average of hydropathy (GRAVY) values was found to be negative for all the fifteen
potato hsfs, suggesting their hydrophilic nature. Of the fifteen hsf genes identified in the potato
genome, five were cloned and sequenced (partially) and their sequences were submitted to NCBI
GenBank and were provided NCBI accession numbers. In response to drought, higher expression of
all the five cloned genes was observed in drought tolerant cultivar- Kufri Jyoti (in leaf as well as
root tissues) as compared to that in drought susceptible cultivar- Kufri Pukhraj. In response to high
temperature stress, although the expression of most of the cloned hsf genes decreased in both high
temperature tolerant (Kufri Surya) as well as susceptible cultivar (Kufri Chandramukhi), however,
the decrease was comparatively more in case of K. Chandramukhi (high temperature susceptible
cultivar) as compared to that in Kufri Surya (high temperature tolerant cultivar). The study indicated
involvement of these hsf genes in imparting tolerance to drought and high temperature stress and
thus, can be utilized for developing more climate resilient potato cultivars.
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