EFFECT OF SUPPLEMENTATION OF DETOXIFIED KARANJ CAKE ON CARCASS CHARACTERISTICS, IMMUNE RESPONSE AND BIOCHEMICAL PROFILE OF BROILER CHICKEN
An experiment was conducted to evaluate the effect of solvent extracted karanj cake (SKC), SKC treated with 2%Â NaOH, 1% NaOH + 1% HCl treated SKC and 3% iso propyl alcohol (IPA) treated SKC on carcass, humoral immune response and biochemical changes in broiler chickens. The experiment comprising of 13 dietary treatments, T1 as control (without karanj cake).Â SKC diets at 3, 6 and 9% levels were designated as T2, T3 and T4; NaOH treated SKC at 3, 6 and 9% levels were designatedÂ as T5, T6 and T7; NaOH + HCl treated SKC at 3, 6 and 9% levels were designated as T8, T9 and T10; while T11, T12 and T13Â comprised of 3%, 6% and 9% IPA treated SKC diets respectively. Six replicates were allocated to each treatment, employing fiveÂ birds/replicate. The experimental period was from day old to 42 d of age. The results revealed that dietary inclusion of either SKCÂ or processed SKC significantly (P<0.05) increased the liver weight, gizzard and giblet weight compared to control group at 42 d ofÂ age. The relative weight of pancreas, abdominal fat, kidney and ready to cook yield were not affected by the dietary inclusion ofÂ either SKC or processed SKC. No significant difference was recorded in haemagglutination inhibition (HI) titers to sheep red bloodÂ cells (SRBC), cell mediated immune response to phytohaemagglutin-P (PHA-P) and relative weights of spleen and bursa at 42 dÂ of age. The serum protein levels significantly (P<0.05) increased in IPA treated SKC, NaOH treated SKC and SKC at 6%Â compared with control. Significantly (P<0.05) lower serum cholesterol level was observed in groups fed 3 & 6% alkali treated SKCÂ compared to control diet. Processing of SKC did not influence the activity of Alkaline phosphatase (ALP) at 3rd and 6th week of age.Â Finally, it was concluded that NaOH, NaOH + HCl and IPA treated SKC (3, 6 and 9%) could be safely included in commercialÂ broilers diet without any adverse effects.