IDENTIFICATION OF COTTON (Gossypium spp.) HYBRIDS AND THEIR PARENTAL LINES USING MICROSATELLITE MARKERS

Abstract

Microsatellite markers were used for fingerprinting of hybrids for assessing variation within parental lines in cotton. Sixteen microsatellite markers were employed for fingerprinting six hybrids and their parental lines. Five SSR markers were found polymorphic across the hybrids and produced unique fingerprint for the six hybrids. These highly informative primers not only differentiated the parent genotypes but also confirmed the parentage of their true hybrids. The microsatellite marker, BNL 3449 amplified alleles specific to differentiate parental lines of NSPHH 5 likewise BNL 3255 primer for NDLHH 240 and WGHH 2, JESPR 148 primer for LAHH 4, BNL1317 for CSH 198 and BNL 3090 for CICR 2. Our findings revealed that SSR procedures are excellent genomic tools for parentage confirmation and hybridity determination, and would also enhance efficiency of our breeding
programmes.