Isolation, purification and pathogenicity of Fusarium oxysporum f. sp. radicis-cucumerinum incited root and stem root of cucumber
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Authors
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Suresh Kumar
Maharana Pratap University of Agriculture and Technology, Udaipur-313001, Rajasthan, India
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N.L. Meena
Maharana Pratap University of Agriculture and Technology, Udaipur-313001, Rajasthan, India
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R.K. Fagodiya
Maharana Pratap University of Agriculture and Technology, Udaipur-313001, Rajasthan, India
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B.L. Fagodia
Maharana Pratap University of Agriculture and Technology, Udaipur-313001, Rajasthan, India
Keywords:
Isolation, Purification, Pathogenicity, Fusarium oxysporum f. sp. radices cucumerinumAbstract
Root and stem rot is more destructive disease of cucumber caused huge economic damage year after year. The present study was undertaken the cucumber root and stem rot disease incited by Fusarium oxysporum f. sp. radicis-cucumerinum leading to rotting of roots, lower stems, crowns and rotting of seeds and seedlings. The pure culture of pathogen was obtained by hyphal tip method. On the basis of morphological and cultural studies, the pathogen was identified as F. oxysporum f. sp. radicis-cucumerinum and further its pathogencity was confirmed. Pathogenicity test of the culture of Fusarium oxysporum f. sp. radicis-cucumerinum was confirmed on young plants of susceptible variety (Cucumber Long Desi) of cucumber in the pot condition. The same symptoms were initiated after 10-12 days of inoculation as rotting and yellowing, sunken stems and wilting. Re-isolation of the pathogen was made on PDA plates and identified it on the basis of culture characteristics of the parent culture.
