CALLUS INDUCTION STUDIES IN SUNFLOWER (HELIANTHUS ANNUUS L.)


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Authors

  • P. DEEPA SANKAR Department of Plant Breeding and Genetics Agricultural College and Research Institute, Madurai - 625 104, Tamil Nadu A
  • N. SUBBRARAMAN Department of Plant Breeding and Genetics Agricultural College and Research Institute, Madurai - 625 104, Tamil Nadu
  • A. THANGA HEMAVATHY Department of Plant Breeding and Genetics Agricultural College and Research Institute, Madurai - 625 104, Tamil Nadu

Keywords:

Sunflower, cotyledon, callus induction, MS media.

Abstract

Germinating seed, hypocotyl, stem bits and cotyledon from germinating seeds were chosen as explants for callus induction in the genotypes CO 2, CO 4, Modem, TCSH 1 and P 6460. Efficient callus indication was observed only from cotyledonary explants tested on MS + 2,4-D 2mg1-1+kinetin 0.5mgl-1 , MS +2,4-D 3 mgl-1 +kinetin 0.5mgl-1 and MS + 2,4-D 2mgl-1 media recorded the lowest callus induction days, the highest per cent callus induction and the highest callus weight. Callus can be best induced in cotyledonary explants of sunflower in MS + 2, 4-D 2 mgl-1 + kinetin 0.25gl-1 + CH 1gl-1 media utilizing genotype CO 4 followed by P 6460.

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Issue

Vol. 27 No. 1 (2006)

Section

Articles

How to Cite

SANKAR, P. D., SUBBRARAMAN, N., & HEMAVATHY, A. T. (2014). CALLUS INDUCTION STUDIES IN SUNFLOWER (HELIANTHUS ANNUUS L.). Annals of Agricultural Research, 27(1). https://epubs.icar.org.in/index.php/AAR/article/view/42249