In-vitro release Properties of Squalene Stabilized by Chitosan-whey Protein isolate

Abstract

The present study was carried out to investigate the
in-vitro release characteristics of encapsulated
squalene as affected by the wall material composition.
Squalene was encapsulated with chitosanwhey
protein isolate as the wall material. The
encapsulation efficiency of the spray dried powder
was found to be 75.40 ± 0.22%. The in-vitro release
profile of microcapsules indicated maximum release
of squalene at the 4th h of digestion (intestinal
phase). Particle size analysis showed that with the
exception in 3rd h, the size of the digested emulsions
exhibited a decreasing trend. Zeta potential values
also exhibited a decreasing trend after the 1st h of
digestion. The results of the study showed that
chitosan-whey protein isolate can be very well
utilised as a wall material for lipophilic compounds
as it confers the final product a higher encapsulation
efficiency and aids in the release of about 59.17%
of squalene from the encapsulated preparation.