Cryopreservation of Brown Trout (Saltno trutta fario) Sperm: The Influence of Extender Composition and Fertilization Procedure

Gopalakrishnan A.; Thakur K.L.; Ponniah A.G.; Kumar K.; Dayal R.

doi:10.56093/ft.v36i2.22860

Cryopreservation of Brown Trout (Saltno trutta fario) Sperm: The Influence of Extender Composition and Fertilization Procedure

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Authors

Gopalakrishnan A. N.B.F.G.R., Cochin Unit, C/o CMFRI
Thakur K.L. Fisheries Directorate, Bilaspur
Ponniah A.G. National Bureau of Fish Genetic Resources, Lucknow
Kumar K. Fisheries Directorate, Bilaspur
Dayal R. National Bureau of Fish Genetic Resources, Lucknow

https://doi.org/10.56093/ft.v36i2.22860

Keywords:

Cryopreservation, brown trout, activator, cryo diluent, extender, milt

Abstract

Seven extenders were tested to check their relative efficiency in cryopreserving the milt of brown trout (Salmo trutta fario). Hatching rates equal to that of control (p > 0.05) were obtained when a cryodiluent containing egg yolk (2%) and dimethylsulphoxide (10%) was used. Removal of coelomic fluid prior to the addition of thawed milt and addition of 5 mM theophylline to the "Dexter" solution enhanced the hatching percentage. The sperm density varied from 9.83 to 18.41 x 109 sperms/ml.

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Submitted

2012-08-04

Published

2025-06-10

Issue

Vol. 36 No. 2 (1999)

Section

Articles

License

How to Cite

A., G., K.L., T., A.G., P., K., K., & R., D. (2025). Cryopreservation of Brown Trout (Saltno trutta fario) Sperm: The Influence of Extender Composition and Fertilization Procedure. Fishery Technology, 36(2). https://doi.org/10.56093/ft.v36i2.22860