Effect of Supplementing Conjugated Linoleic Acid Producing Bifidobacterial Strains on In vitro Rumen Fermentation Attributes


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Authors

  • Neeru Jaglan
  • Sachin Kumar
  • Prasanta Kumar Choudhury
  • Bhawna Tyagi
  • P. S. Banakar
  • Nitin Tyagi
  • Amrish Kumar Tyagi

Keywords:

Bifidobacteria, Conjugated linoleic acid, IVDMD, Murrah buffaloes, Rumen fermentation

Abstract

The present study was undertaken to evaluate the effects of supplementation of four conjugated linoleic acid (CLA) producing strains of Bifidobacteria isolated from rumen fluid samples of Murrah buffalo (Bubalus bubalis) on ruminal fermentation attributes based on a basal diet having roughage: concentrate in a ratio of 70:30. Results of the study showed that dosing Bifidobacterium strains (109 cells/ml) separately into in vitro incubation tubes neither affect the total gas nor methane (CH4) production after 24 h of incubation. Digestibility parameters showed no significant (P>0.05) changes with respect to in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), or total ammonia-N content of fermentation fluid. However, volatile fatty acid (VFA) concentration was observed to be significantly increased (P<0.05) in the treatment samples with bacterial strains NB-191 and NB-184. While analyzing CLA content of incubation medium, a significant increase in the value was observed as compared to control tubes. Therefore, it can be concluded that the addition of potential CLA producing Bifidobacterial isolates to mixed rumen culture increased the CLA and VFA content without affecting digestibility and fermentation parameters.

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Submitted

10-08-2020

Published

01-10-2020

Issue

Vol. 37 No. 2 (2020)

Section

Ruminant

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Copyright remains with the society and author jointly. However, material can be used for research, teaching and to achieve goals of the society.

How to Cite

Jaglan, N., Kumar, S., Choudhury, P. K., Tyagi, B., Banakar, P. S., Tyagi, N., & Tyagi, A. K. (2020). Effect of Supplementing Conjugated Linoleic Acid Producing Bifidobacterial Strains on In vitro Rumen Fermentation Attributes. Indian Journal of Animal Nutrition, 37(2). https://epubs.icar.org.in/index.php/IJAN/article/view/103379