Effect of Tulsi (Ocimum sanctum) Leaf and Synbiotic Powder on Growth Performance, Nutrient Utilization, Carcass and Blood Biochemical Parameters in Broilers
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Keywords:
Broiler performance, Carcass, Nutrient utilization, Synbiotic, Tulsi.Abstract
A six-week feeding trial was followed by a metabolic trial using 120 broiler chicks (Cob-400) in a completely randomized block design to assess the effect of dietary supplementation with tulsi leaf and synbiotic powder as feed additives on growth performance of broilers. The four dietary treatments were designated as TC control group (basal diet), T1 supplemented with tulsi @ 0.5%, T2 supplemented with synbiotic @ 0.05%, T3 supplemented with tulsi @ 0.5% and synbiotic @ 0.05% of feed. Growth performance and carcass yields were significantly higher (P<0.01) in dietary treatment with supplementation of tulsi leaf and synbiotic as compared to control. Significantly higher body weight, weight gain, daily weight gain, protein efficiency ratio and performance index were observed in the T3 group supplemented with a combination of tulsi and synbiotic @ 0.5 percent and 0.05 percent, respectively. Similarly, dietary supplementation of tulsi leaf and synbiotic in combination significantly reduced serum triglyceride and cholesterol level. Whereas, dietary inclusion of tulsi leaf alone and in combination with synbiotic reduced feed intake and feed conversion ratio. However, dry matter digestibility, nitrogen balance, eviscerated weight, heart, liver, gizzardweights, total protein, albumin, globulin, haemoglobin and packed cell volume did not differ in treatment groups. The mortality rate was found to be lower in the treatment groups when compared to the control group. Based on the findings of this study, it is concluded that supplementing tulsi leaf at 0.5 percent with synbiotic powder at 0.05 percent improved the growth performance, nutrient utilization, carcass traits and blood haemato-biochemical parameters of broiler chicks without any negative effects.
References
A.O.A.C. 2016. Official method of analysis. 15th Edn., Association of Official Analytical Chemists, Washington, D.C., U.S.A.
Abdel, H.H.M., Saleh, E.S., Tawfeek, S.S., Youssef, I.M. and Abdel-Daim, A.S. 2017. Effects of probiotic, prebiotic, and synbiotic with and without feed restriction on performance, hematological indices and carcass characteristics of broiler chickens. Asian-Australasian Journal of Animal Sciences. 30(5):672.
Abdel, R.S.M., Abd-Allah, S.M. and Hassanein, K.M. 2012.The effects of prebiotic, probiotic and synbiotic supplementation on intestinal microbial ecology and histomorphology of broiler chickens. International Journal for Agro Veterinary and Medical Sciences. 6(4):277-289.
Apata, D. F. 2008. Growth performance, nutrient digestibility and immune response of broiler chicks fed diets supplemented with a culture of Lactobacillus bulgaricus. Journal of the Science of Food and Agriculture. 88(7):1253-1258.
Armstrong, D.W. and Yamazaki, H. 1986. Natural flavours production: a biotechnological approach. Trends in Biotechnology.4(10):264-268.
Ashayerizadeh, A., Dabiri, N., Ashayerizadeh, O., Mirzadeh, K.H., Roshanfekr, H. and Mamooee, M. 2009. Effect of dietary antibiotic, probiotic and prebiotic as growth promoters, on growth performance, carcass characteristics and hematological indices of broiler chickens. Pakistan Journal of Biological Sciences.12: 52-57.
Awad, W.A., Ghareeb, K., Abdel-Raheem, S. and Bohm, J. 2009. Effect of dietary inclusion of probiotic and synbiotic on growth performance, organ weights and intestinal histomorphology of broiler chickens. Poultry Science.88 (1):49-56.
Bhosale, D.S., Bhagwat, S.R., Pawar, M.M. and Kulkarni, R.C. 2015.Comparative Efficacy of Dietary addition of Ocimum sanctum (Tulsi) leaf powder and vitamin E on broiler performance. Indian Journal of Animal Nutrition. 32(3):348-350.
BIS. 1992. Poultry Feed Specification: IS: 1374 (4th revision). Bureau of Indian Standards, Manak Bhavan, 9 Bhadurshah Zafar Marg, New Delhi-110002, India.
Biswas, A.K., Rahman, M.M., Hassan, M.Z., Sultana, S., Rahman, M.M. and Mostofa, M. 2017. Effect of tulsi (Ocimum sanctum) leaves extract as a growth promoter in broiler productio. Asian Journal of Medical and Biological Research. 3(2):226-232.
Capcarova, M., Weiss, J., Hrncar, C., Kolesarova, A. and Pal, G. 2010. Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens. Journal of Animal Physiology and Animal Nutrition. 94(5):215-224.
Bozkurt, M. Kuçukyılmaz, K., Çatlı, A. U. and Cinar, M. 2009.The effect of single or combined dietary supplementation of prebiotics, organic acid and probiotics on performance and slaughter characteristics of broilers. South African Journal of Animal Science. 39(3):197-205.
Das,O., Patil, S.S., Savsani, H.H., Padodara, R.J., Garg, D.D., Marandi, S. and Barad, N. 2016. Effect of dietary prebiotics, probiotics and synbiotics as feed additives on blood profile and broiler performance. International Journal of Science, Environment and Technology. 5(5):3546-3552.
Dev, K., Mir, N.A., Biswas, A., Kannoujia,J., Begum,J., Kant, R. and Mandal, A. 2020. Dietary synbiotic supplementation improves the growth performance, body antioxidant pool, serum biochemistry, meat quality, and lipid oxidative stability in broiler chickens. Animal Nutrition. 6(3):325-332.
Dizaji, B.R., Hejazi, S. and Zakeri, A. 2012. Effects of dietary supplementations of prebiotics, probiotics, synbiotics and acidifiers on growth performance and organs weights of broiler chicken. European Journal of Experimental Biology. 2(6):2125-2129.
Gupta, G. and Charan, S. 2007. Exploring the potentials of Ocimum sanctum (Shyama Tulsi) as a feed supplement for its growth promoter activity in broiler chickens. Indian Journal of Poultry science. 42(2):140-143
Hasan, M.N., Mostofa, M., Sorwar, M.G., Hasan, M.T., Das, K. and Hossain, D.M.N. 2016. Effects of Tulsi leaf extract on body weight gain in broiler production. Bangladesh Journal of Veterinary Medicine. 14(1):21-25.
Hassanpour, H., Zamani, A.K., Moghaddam,Khosravi M. and Mayahi, M. 2013. Effects of synbiotic on the intestinal morphology and humoral immune response in broiler chickens Livestock Science.153(1-3):116-122.
Islam, M.Z., Khandaker,Z.H. Chowdhury,S.D. and Islam., K.M.S. 2008. Effect of citric acid and acetic acid on the performance of broilers. Journal of Bangladesh Agricultural University. 6:315-320.
Julendraa, H., Sofyanb, A., Karimy, M.F., Abinawantoa and Yasman 2020. Nutrient utilizations and intestinal morphology of broilers treated with Lactobacillus plantarum akk30 – oligosaccharides synbiotic. Journal of Animal Science.43(2):158-168.
Kramer, C.Y. 1956. Extension of multiple range tests to group means with unequal numbers of replications. Biometrics. 12: 307-10.
Lanjewar, R.D., Zanzad, A.A., Ramteke, B.N. and Deshmukh, G.B. 2008. Effect of dietary supplementation of tulsi (Ocimum sanctum) leaf powder on the growth performance and serum lipid profile in broilers. Indian Journal of Animal Nutrition. 25 (4):395-397.
Min, Y.N., Yang, H.L., Xu, Y.X. and Gao, Y.P. 2016. Effects of dietary supplementation of synbiotics on growth performance, intestinal morphology, sIgA content and antioxidant capacities of broilers. Journal of Physiology and Animal Nutrition.100:1073-1080
Nagar, 2021. Effect of turmeric (Curcuma longa) powder and synbiotic as alternative to antibiotic growth promoter on the performance, carcass characteristics and nutrient utilization in broiler chicks. Thesis submitted to RAJUVAS Bikaner.
Nagar, M.K., Shende, K., Dhuria, R.K., Manzer, H. and Surendra, 2021. Effect of turmeric (curcuma longa) powder and synbiotic as alternative to antibiotic growth promoter on the growth performance and mortality of broiler chicks. Journal of Animal Research. 11(1): 167-172.
Popovic, S.J., Kostadinovic, L.M., Puvaca, N.M., Levic, J.D., Duragic, O.M., Kokic, B.M. and Vranjes, M.V. 2015. Effect of synbiotic on growth and antioxidant status of blood in broiler chicken. Food and Feed Research. 42(2):163-169.
Prajapat, 2016. Effect of supplementation of Ocimum sanctum (tulsi) and Trigonella foenum-graecuml. (fenugreek) on the performance of broiler chicks. Thesis submitted to RAJUVAS, Bikaner.
Salah, A.S., El-Tarabany, M.S. and Ali, M.A. 2018. Impact of dietary supplementation with a synbiotic, organic acids or their combination on growth performance, carcass traits, economic efficiency, jejunum histomorphometry and some blood indices of broiler chickens. Animal Production Science. 59(7): 1318-1326.
Salehimanesh, A., Mohammadi, M. and Roostaei-Ali M.M. 2016. Effect of dietary probiotic, prebiotic and synbiotic supplementation on performance, immune responses, intestinal morphology and bacterial populations in broilers. Journal of Physiology and Animal Nutrition.100:694-700.
Sharifi, M.R., Shams-Shargh, M., Dastar, B. and Hassani, S. 2011. The effect of dietary protein levels and synbiotic on performance parameters, blood characteristics and carcass yields of Japanese quail (Coturnixcoturnix Japonica). Italian Journal of Animal Science.10(1): 17-21.
Shende, K., Dhuria, R.K., Goklaney, D. and Barolia, Y.K. 2021a. Effect of supplementation of tulsi (Ocimum sanctum) leaf and ginger (Zingiber officinale) powder as feed additive on daily weight gain, comparative economics and mortality percent of broiler chicks. International Journal of Bio-resource and Stress Management. 12(1):001-006.
Shende, K., Dhuria, R.K., Goklaney, D. and Barolia, Y.K. 2020a. Effect of tulsi (Ocimum sanctum) leaf and ginger (Zingiber officinale) powder as feed additives on haemato-biochemical parameters of broiler chicks. Journal of Animal Research.10(6):1057-1062.
Shende, K., Dhuria, R.K., Meel, M., Nagar, M.K. and Jediya, H.K. 2021b. Effect of turmeric (Curcuma longa) powder and synbiotic as alternative to antibiotic growth promoter on haemato-biochemical parameters, comparative economics and mortality of broiler chicks. International Journal of Bio-resource and Stress Management.12(1):125-130.
Shende, K.A., Dhuria, R.K. and Sharma, T. 2020b. Effect of Ocimum sanctum (tulsi) leaf and Zinziber officinale (ginger) powder as feed additives on growth performance of broiler chicks.Veterinary Practitioner. 21(1): 139-141.
Singh, A., Pranav, D. and Jagdish, P. 2014. Effect of Ocimum sanctum (Tulsi) on productive performance and blood biochemistry of broilers. Research Opinions in Animal and Veterinary Sciences. 4(3):142-144.
SPSS 2018. Statistical package for social sciences, Version 20. Procedure and Facilities for Research. McGraw Hill Book Co., New York.
Swathi, B., Gupta, P.S.P. and Nagalakshmi, D. 2012. Effect of tulsi (Ocimum sanctum) and turmeric (Curcuma longa) on broiler performance and blood constituents during heat stress in broilers. International Journal of Pharma and Bio Sciences. 3(3):446-453.
Tanwar, R. 2018. Effect of aloe vera (Aloe barbadensis) and tulsi (Ocimum sanctum) supplementation on performance of broiler chickens. Thesis submitted to RAJUVAS, Bikaner.
Vasanthakumar, P., Sasikumar, P., Pangayarselvi, B., Chandrasekaran, D., Doraisamy, K.A. Senthilkumar and Purushothaman, M.R. 2013. Performance of broiler chicken fed tulsi leaf powder and leaf extract supplemented diets during summer to alleviate heat stress. Indian Journal of Animal Science. 83:930-931.
Wenk, C. 2003. Herbs and botanicals as feed additives in monogastric animals. Asian-Australasian Journal of Animal Science. 16(2):282-289.
Yadav, V.S. 2018. Effect of tulsi (ocimum sanctum) and organic acid supplementation on performance of broiler chickens. Thesis submitted to RAJUVAS, Bikaner.
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