Effect of Feeding Neem and Papaya Leaf Meal as Feed Additive on Performance, Immune response, Antioxidant status and Serum biochemical parameters in Broilers
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Authors
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Shiwani Singh
Mumbai Veterinary College, Parel, Mumbai
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Ankur Khare
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R.P.S. Baghel
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Sunil Nayak
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Prital Bhujbal
Keywords:
Antioxidant status, Broilers, Immune response, Neem Leaf Meal, Papaya Leaf Meal, Serum biochemicalAbstract
Objective of research was to investigate the effects of supplementing neem and papaya leaf meal, individually and in combination on performance, immune response, antioxidant status and serum biochemical parameters of broiler production. 220, day-old Cobb commercial broiler chicks were randomly divided into 11 treatments with 4 replicates having 5 chicks in each. In treatment groups along with basal diet, neem and papaya leaf meal were incorporated in the diet at two different levels, individually (0.5 and 1.0% of diet) and in three different combinations (1:1, 1:2 and 2:1 part of NLM and PLM @ 1.0 and 2.0% of diet). Individual body weight, weight gain and feed intake of broilers were recorded at weekly interval and, there after feed conversion ratio (FCR), feed efficiency ratio (FER) and performance index (PI) was calculated. At the end of trial (42 days), 02 birds from each replicate were sacrificed for performing anti-oxidant property in liver tissue and breast muscle. Serum biochemical parameters were estimated at the end of the experiment. Humoral immunity towards Newcastle disease virus was measured on 30th and 42nd day of feeding trial by haemagglutination inhibition (HI) test. Results pointed out that supplementation of neem and papaya leaf meal in 1:1 ratio at 1.0%, in 1:2 ratio at 1.0 and 2.0% of total broiler diet significantly (P<0.05) enhanced their overall performance (body weight, weight gain, feed intake, feed conversion ratio, feed efficiency ratio and performance index). Final results indicated that immune responses (humoral), antioxidant status of liver and breast muscle tissues improved significantly (P<0.05) in broilers fed on neem and papaya leaf meal supplemented groups. However, serum concentration of total triglycerides, total cholesterol, LDL cholesterol significantly (P<0.05) reduced and increased HDL cholesterol with the supplementation.
References
Adeyemo, G.O. and Akanmu, M.A. (2012). Effects of Neem (Azadirachta indica) and pawpaw (Carica papaya) leaves supplementation on performance and carcass characteristics of broilers. International Journal of Current Research, 4: 268-271.
Afolabi, I.S., Marcus, G.D., Olanrewaju, T.O. and Chizea, V. (2011). Biochemical effect of some food processing methods on the health-promoting properties of under-utilized Carica papaya seed. Journal of Natural Products, 4: 17-24.
Anibijuwon, I.I. and Udeze, O.A. (2009). Antimicrobial Activity of Carica Papaya (Pawpaw Leaf) on Some Pathogenic Organisms of Clinical Origin from South-Western Nigeria. Ethnobotanical Leaflets, 13: 850-864.
Ansari, J., Khan, S.H., UlHaq, A. and Yousaf, M. (2012). Effect of the levels of Azadirachta indica dried leaf meal as phytogenic feed additive on the growth performance and haemato-biochemical parameters in broiler chicks. Journal of Applied Animal Research, 40(4): 336-345.
Buragohain, R. (2016). Growth performance, nutrient utilization and feed efficiency in broilers fed Tithonia diversifolia leaf meal as substitute of conventional feed ingredients in Mizoram. Veterinary World, 9(5): 444-449.
Deka, J., Mahanta, J.D., Kalita, K.P., Choudhury, D., Ahmed, H.F. and Tamuly, S. (2019). Effect of dietary supplementation of Neem (Azadirachta indica) leaf powder on the performance of commercial broiler chicken. Journal of Entomology and Zoology Studies, 7(3): 658-663.
Duncan, D.B. (1955). Multiple range and multiple F tests. Biometrics, 11: 1-42.
Durrani, F.R., Sultan, A., Akhtar, S., Jan, M., Chand, N. and Durrani, Z. (2008). Immunomodulatory and growth-promoting effects of neem leaves infusion in broiler chicks. Sarhad Journal of Agriculture, 24(4): 655-659.
Ebrahimi, M., Maroufyan, E., Shakeri, M., Oskoueian, E., Soleimani, A.F. and Goh, Y.M. (2015). Papaya leaf in broiler chicken feed can reduce lipid peroxidation of meat. In: International Conference on Animal and Veterinary Medicine, Boston, USA, 20-21, April 2015.
Garba, S., Mera, U.M., Garba, H.S., Musa, U., Jimoh, A.A. and Raji, A.A. (2013). Effect of garlic and neem leaf aqueous extracts on immune response of broilers to live Newcastle disease vaccine. Scientific Journal of Veterinary Advances, 2: 16-20.
Kadiri, O., Olawoye, B., Fawale, O.S. and Adalumo, O.A. (2016). A review: Nutraceutical and antioxidant properties of the seeds, leaves and fruits of Carica papaya: Potential relevance to human diet, the food industry and the pharmaceutical industry. Turkish Journal of Agriculture - Food Science and Technology, 4(12): 1039-1052.
Kähkönen, M.P., Hopia, A.I. and Heinonen, M. (2001). Berry phenolics and their antioxidant activity. Journal of Agricultural and Food Chemistry, 49: 4076-4082.
Kale, B.P., Kothekar, M.A., Tayade, H.P., Jaju, J.B. and Mateenuddin, M. (2003). Effect of aqueous extract of Azadirachta indica leaves on hepatotoxicity induced by antitubercular drugs in rats. Indian Journal of Pharmacology, 35: 177-180.
Kamal, A.B.M.M., Boby, F., Shahjada, Z., Lutfuzzaman, A.K.M., Motin, A.A., Alam, K.J. and Mostofa, M. (2015). Effects of Neem, Nishyinda and Papaya leaves as growth promoters in broiler chicks. International Journal of Natural and Social Sciences, 2: 49-55.
Mali, P.D., Adangale, S.B., Sharma, N., Fulpagare, Y.G. and Patale, S.S. (2020). Effect of Supplementation of Neem (Azadirachta indica) Leaf Powder on Growth Performance and Carcass Characteristics of Broilers. International Journal of Current Microbiology and Applied Sciences, 9(10): 70-74.
Obikaonu, H.O., Okoli, I.C., Opara, M.N., Okoro, V.M.O., Ogbuewu, I.P., Etuk, E.B. and Udedibie, A.B.I. (2011). Haematological and serum biochemical indices of starter broilers fed neem (Azadirachta indica) leaf meal. Online Journal of Animal and Feed Research, 1(4): 150-154.
Ogbuewu, I.P., Okoli, I.C. and Iloeje, M.U. (2010). Evaluation of toxicological effects of leaf meal of an ethnomedicinal plant-neem on blood chemistry of puberal Chinchilla rabbit does. Report and Opinion, 2(2): 29-34.
Ojiako, C.M., Okoye, E.I., Oli, A.N., Ike, C.J., Esimone, C.O. and Attama, A.A. (2019). Preliminary studies on the formulation of immune-stimulating complexes using saponin from Carica papaya leaves. Heliyon, 5(6): e01962.
Oloruntola, O.D., Agbede, J.O., Ayodele, S.O. and Oloruntola, D.A. (2018). Neem, pawpaw and bamboo leaf meal dietary supplementation in broiler chickens: Effect on performance and health status. Journal of Food Biochemistry, 43(2): e12723.
Onyimonyi, A.E. and Onu, E. (2009). An assessment of pawpaw leaf meal as protein ingredient for finishing broiler chickens. International Journal of Poultry Science, 8(10): 995-998.
Poulter, N.H. and Caygill, J.C. (1985). Production and utilization of papain—a proteolytic enzyme from Carica papaya latex. Tropical Science, 25: 123-137.
Rahman, M.A., Ali, M.A., Saha, B.K., Hasan, M.A.A., Rahman, M.A. and Mostofa, M. (2015). Use of neem leaf and ginger extracts for cost effective broiler production. International Journal of Natural and Social Sciences, 2: 11-16.
Rama Rao, S.V., Raju, M.V.L.N., Prakash, B., Rajkumar, U. and Reddy, E.P.K. (2019). Effect of supplementing moringa (Moringa oleifera) leaf meal and pomegranate (Punica granatum) peel meal on performance, carcass attributes, immune and antioxidant responses in broiler chickens. Animal Production Science, 59: 288-294.
Subapriya, R. and Nagini, S. (2005). Medicinal properties of neem leaves: a review. Current Medicinal Chemistry Anticancer Agents, 5: 149-156.
Ubua, J.A., Ozung, P.O. and Inagu, P.G. (2019). Dietary inclusion of neem (Azadirachta indica) leaf meal can influence growth performance and carcass characteristics of broiler chickens. Asian Journal of Biological Science, 12: 180-186.
Vergara-Jimenez, M., Almatrafi, M.M. and Fernandez, M.L. (2017). Bioactive components in Moringa oleifera leaf protect against chronic disease. Antioxidants, 6(4): 91.
Witte, V.C., Krause, G.F. and Bailey, M.E. (1970). A new extraction method for determining 2-thiobarbituric acid value of pork and beef during storage. Journal of Food Science, 35: 582-585.
Wyllie, D. and Chamanga, P.J. (1979). Cassava leaf meals in broiler diets. Tropical Animal Production, 4: 232-240.
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