Effect of Humic Substance Supplementation on Performance and Gut Health in Broilers
234 / 12
Authors
-
Jyothi Chamarthi
-
N Muwel
Nanaji Deshmukh Veterinary Science University, Jabalpur
-
Sunil Nayak
-
Ankur Khare
-
Rahul Sharma
-
Ajay Ray
-
Anil Shinde
-
Nidhi Gupta
Keywords:
Broiler, Histomorphometry, Humic Substance, Nutrient Utilization PerformanceAbstract
The study was conducted to investigate the effect of dietary supplementation of humic substance (HS) on performance of broilers. 120, day old broiler chicks were randomly distributed into 6 experimental groups with 5 replicates of 4 chicks each. The standard broiler diets (CON) were formulated as per commercial chick feed specification (2011). Dietary treatment AGP was same as CON with addition of AGP whereas, in HS0.1, HS0.2, HS0.3 and HS0.4 replacing antibiotic with HS @ 1, 2, 3 and 4 g/kg feed respectively. Individual body weight and feed intake of broilers were recorded and thus feed to gain ratio and EPEI % was calculated. Birds were sacrificed to estimate carcass traits, intestinal histomorphometry and caecal microbial count. Immunological response in broilers and liver function tests (SGOT and SGPT) were estimated. The performance of broilers (0-6 weeks) indicated that supplementation of HS0.4 in the broiler diet significantly (p<0.05) improved their body weight. Better feed to gain ratio and EPEI % was observed with HS0.2 compared to CON. Crude protein and crude fat utilization improved significantly (p<0.05) in broilers fed with HS0.2 and HS0.4. Supplementation of HS significantly (p<0.05) improved intestine villus height, crypt depth and VH:CD ratio. Caecal E. coli count was lowered, humoral immune response, weight of bursa of fabricius, spleen, serum IgG and IL-10 levels were increased in HS supplemented groups compared to CON. HS inclusion had no effect on the carcass traits, nutrient composition of meat and liver function tests. Lower feed cost per kg weight gain and improved economic efficiency was observed in broilers fed on diets supplemented with HS0.2. It can be concluded that supplementation of HS0.2 in feed can be used as an economically efficient alternative to antibiotic growth promoters in broilers diet.
References
Abdel-Mageed, M.A.A. 2012. Effect of dietary humic substances supplementation on performance and immunity of Japanese quail. Egyptian Poultry Science Journal. 32(3): 645-660.
Ahfeethah, F., Elazomi, A. and Kammon, A.2023. Effect of humic acid and probiotics on immunity of broiler chickens. Open Veterinary Journal. 13(7): 839-845.
Anadon, A. 2006. WS14 the EU ban of antibiotics as feed additives (2006): alternatives and consumer safety. Journal of Veterinary Pharmacology and Therapeutics. 29(1): 41-44.
AOAC. 2012.Official Methods of Analysis, 19thEdn.Association of Analytical Chemists, Washington, D.C,USA.
Arif, M., Rehman, A., Saeed, M., Abd El-Hack, M.E., Arain, M.A., Haseebarshad, M., Zakria, H.M. and Abbasi, I.H. 2016. Impacts of dietary humic acid supplementation on growth performance, some blood metabolites and carcass traits of broiler chicks. Indian Journal of Animal Science. 86(9): 1073-1078.
Aristimunha, P.C., Mallheiros, R.D., Ferket, P.R., Cardinal, K.M., Moreira Filho, A.L.D.B., Santos, E.T., Cavalcante, D.T. and Ribeiro, A.M.L. 2020. Effect of dietary organic acids and humic substance supplementation on performance, immune response and gut morphology of broiler chickens. Journal of Applied Poultry Research. 29(1): 85-94.
Bezuglova, O. and Klimenko, A. 2022. Application of humic substances in agricultural industry. Agronomy. 12(3): 584.
Commercial chick feed specification. 2011. Feed specification for VENCOBB 400 broilers, Venkateshwara Hatcheries Private Limited, Pune, India.
Dominguez-Negrete, A., Gomez-Rosales, S., Angeles, M.D.L., Lopez-Hernandez, L.H., Reis-de Souza, T.C., Lopez-Garcia, Y., Zavala-Franco, A. and Tellez-Isaias, G.2019. Effect of the addition of humic substances as growth promoter in broiler chickens under two feeding regimens. Animals. 9(12): 1101.
Drury, R.A.B. and Wallington, E.A. 1980. Carleton’s Histological Technique, 5th Edn. Oxford University Press, pp 99-113.
Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42.
ELnaggar, A.S. and El-Kelawy, M.I. 2018. Effect of humic acid supplementation on productive performance, blood constituents, immune response and carcass characteristics of sasso chicken. Egyptian Journal of Animal Production. 55(1): 75-84.
Ghazalah, A.A., El-Tahawy, W.S., Ghalwash, A.A. and Elnaggar, A.S. 2022. Productive and physiological response of broiler chicks to dietary humic acid. Egyptian Poultry Science Journal. 42(2): 157-170.
Global Agricultural Information Network. 2021. India’s poultry market - a snapshot of 2020-21 online https://www.fas.usda.gov/data/india-indias-poultry-market-snapshot-2020-21
Gonzelez, M.J.A., Michel, M.A., Baxter, M.F.A., Tellez, G., Moore, P.A., Solis, C.B., Patlan, D.H., Guzman, R.M., Velasco, X.H., Latorre, J.D., Hargis, B.M., Rosales, S.G. and Isaias, G.T. 2018. Effect of humic acids on intestinal viscosity, leaky gut and ammonia excretion in a 24 hr feed restriction model to induce intestinal permeability in broiler chickens. Animal Science Journal. 89(7): 1002-1010.
Hammod, A.J., Zeny, Z.A.H., Mahdi, A.S. and Alfertosi, K.A. 2021. Probiotic and humic acid as feed additives and their effects on productive and economic traits of broiler. Indian Journal of Ecology. 48(13): 35-37.
Hascik, P., Arpasova, H., Pavelkova, A., Bobko, M., Cubon, J. and Bucko, O. 2018. Chemical composition of chicken meat after application of humic acid and probiotic Lactobacillus fermentum. Potravinarstvo Slovak Journal of Food Sciences. 12(1): 694–700.
Hubbard, S.A.S.1999. Broiler management guide, Hubbard SAS, Quintin, France.
Huck, J.A., Porte, N., and Bushell, M.E. 1991. Effect of humates on microbial activity. General Microbiology. 137: 2321-2329.
Kati, S.M. 2018. Effect of addition of different levels of humic acid to drinking water on productive and carcass characteristics of broiler chick. Journal of Kerbala for Agricultural Sciences. 5(3): 1-13.
Khalaquzzman, M. 2022. Effect of humic acid, probiotics and their combination on growth performance, immune response and carcass characteristics of broiler. MS thesis (Animal Nutrition), Sher-e-Bangla Agricultural University, Dhaka.
Korsakov, K., Simakova, I., Vasilyev, A., Lifanova, S. and Gulyaeva, L. 2019. The effect of humic acids on the natural resistance of the body of broiler chickens and the quality of their meat. Agronomy Research. 17(S2): 1356-1366.
Lala, A.O., Kwelum, N.O., So, A.O.O., Ajao, A.O. and Degbenjo, A.A.A. 2017. Response of broiler chickens to varying dosage of humic acid in drinking water. National Animal Production Research Institute. 29(1): 288-294.
Lopez, G.Y.R., Gomez, R.S., Angeles, M.D.L., Jimenez, S.H., Merino, G.R. and Tellez, I.G. 2023. Effect of the addition of humic substances on morphometric analysis and number of goblet cells in the intestinal mucosa of broiler chickens. Animals. 13(2): 212-227.
Ma, J., Mahfuz, S., Wang, J. and Piao, X. 2021. Effect of dietary supplementation with mixed organic acids on immune function, antioxidative characteristics, digestive enzymes activity and intestinal health in broiler chickens. Frontiers in Nutrition. 8: 673316.
Marcincak, S., Semjon, B., Marcincakova, D., Reitznerova, A., Mudronova, D., Vaskova, J. and Nagy, J. 2023. Humic substances as a feed supplement and the benefits of produced chicken meat. Life. 13(4): 927.
Markey, B., Leonard, F., Archambault, M., Cullinane, A., Maguire, D., Edwards, R. and Hewat, C. 2013. Clinical Veterinary Microbiology, 2nd Edn.Mosby Elsevier, London, pp 215-239.
Mehdi, A. and Hasan, G. 2012. Immune response of broiler chicks fed yeast derived mannan oligosaccharides and humate against Newcastle disease. World Applied Sciences Journal. 18(6): 779-785.
Mudronova, D., Karaffova, V., Pesulova, T., Koscova, J., Marussakova, I.C., Bartkovsky, M., Sevsikova., Z. and Marcincak, S. 2020. The effect of humic substances on gut microbiota and immune response of broilers. Food and Agricultural Immunology. 31(1): 137-149.
Nagaraju, R., Reddy, B.S., Gloridoss, R., Suresh, B.N. and Ramesh, C. 2014. Effect of dietary supplementation of humic acids on performance of broilers. Indian Journal of Animal Science. 84(4): 447-452.
Office International des Epizooties. 2004. Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). Office International des Epizooties, Paris, pp 258-269.
Omidiwura, B.R., Agboola, A.F., Emeruwa, O.M. and Awofodu, O.O. 2021. Effect of humic acid supplementation on growth response, gut morphology and microbial load in broiler chickens. Slovak Journal of Animal Science. 54(4): 176-185.
Panda, A.K., Rama Rao, S.V., Raju, M.V.L.N. and Shyam Sunder, G. 2009. Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Australasian Journal of Animal Sciences. 22(7): 1026-1031.
Pistova, V., Arpasova, H. and Hrncar, C. 2016. The effect of the humic acid and garlic (Allium sativum) on performance parameters and carcass characteristic of broiler chicken. Journal of Central European Agriculture. 17(4): 1168-1178.
Reitman, S. and Frankel, S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology. 28(1): 56-63.
Saleh, A.A., Yassin, M., El-Naggar, K., Alzawqari, M.H., Albogami, S., Soliman, M., Shurky, M., Farrag, F. and Kirrella, A.A. 2022. Effect of dietary supplementation of humic acid and lincomycin on growth performance, nutrient digestibility, blood biochemistry and gut morphology in broilers under clostridium infection. Journal of Applied Animal Research. 50(1): 440-452.
Samudovska, A.H., Bujnak, L. and Zigo, F.2022. Carcass characteristic, caecal metabolites and dropping quality in broiler chickens fed diets containing a humic substances. Asian Journal of Agriculture and Food Sciences. 10(6): 133-138.
Snedecor, G.W. and Cochran, W.G. (1994). Statistical Method, 8th Edn. The lowa state university press, Ames, Lowa, USA.
Taklimi, S.M., Ghahri, H. and Isakan, M.A. 2012. Influence of different levels of humic acid and esterified glucomannan on growth performance and intestinal morphology of broiler chickens. Journal of Agricultural Science. 3(5): 663-668.
Zhang, A.R., Pirzado, S.A., Liu, G.H., Chen, Z.M., Chang, W.H., Cai, H.Y., Bryden W.L. and Zheng, A.J. 2020. Dietary supplementation with sodium humate improves egg quality and immune function of laying hens. Journal of Applied Animal Nutrition. 8(2): 93-99.
Downloads
Submitted
Published
Issue
Section
License
Copyright remains with the society and author jointly. However, material can be used for research, teaching and to achieve goals of the society.
