In-Vitro, In-Situ and In-Vivo Evaluation of Alfalfa Residue Based Total Mixed Ration Feed Blocks for Sheep
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Keywords:
Alfalfa residue, In-Situ, In-Vitro, lambs, Total Mixed Ration feed blockAbstract
This study explored the potential of Alfalfa nutraceutical residue (ANR) as a protein rich concentrate for growing lambs by conducting In-Vitro, In-Situ and In-Vivo methods. The ANR was high in crude protein (49.25%) and low in crude fiber (0.91%), with Ca: P ratio of 5.67:1.47. In-Situ protein degradability was very slow (RDP:UDP ratio was 10.49:89.51) and the Metabolizable energy (ME) of 4.3 MJ/kg was found. An In-Vivo feeding trial was conducted with 18 male lambs (3-4 months old) divided into three groups: T1 (Control) received Total Mixed Ration feed block (TMRfb) containing groundnut haulms and compounded feed mixture (50:50); T2 replaced 25% of crude protein (CP) with ANR, and T3 replaced 50%. All rations were iso-nitrogenous. Results showed that lambs in T2 (4.28%) and T3 (4.19%) had higher dry matter intake (DMI) than T1 (4.04%), but not varied significantly. Feed efficiency was similar across groups (5.41 to 5.81), but average daily gain (ADG) was higher in T2 (123.69 g/day) compared to T3 (111.29 g/day) and T1 (106.9 g/day). Nutrient digestibility and nitrogen balance were comparable among groups. Therefore, ANR can effectively replace up to 50% of crude protein in lamb diets, promoting growth without negatively affecting feed efficiency or nutrient digestibility.
References
AOAC. 2016. Official methods of analysis of the Association of Official Analytical Chemists, 20th Ed., AOAC Inc., Washington DC.
Arlabosse, P. and Blanc, M., 2011, October. Thermo-mechanical fractionation of green biomass: optimisation of the processing conditions according to the water potential of the tissue. In European Drying Conference, pp.26-28.
Babiker, E.E., Juhaimi, F.A., Ghafoor, K. and Abdoun, K.A., 2017. Comparative study on feeding value of Moringa leaves as a partial replacement for alfalfa hay in ewes and goats. Livestock Science, 195: 21-26.
Damborg, V.K., Jensen, S.K., Weisbjerg, M.R., Adamsen, A.P. and Stodkilde, L. 2020. Screw-pressed fractions from green forages as animal feed: Chemical composition and mass balances. Animal Feed Science and Technology, 261: 114401.
Garg, M.R., Sherasia, P.L. and Bhanderi, B.M., 2013. Quality control manual for cattle feed plants. Animal Nutrition Group, National Dairy Development Board, Anand-388, 1: 8.
Gaweł, E. and Grzelak, M. 2014. Protein from Alfalfa in animals supplement diet. Journal of Food, Agriculture and Environment, 12 (2): 314-319.
Hawkins, C. and Yu, L.X. 2018. Recent progress in Alfalfa (Medicago Sativa L.)
genomics and genomic selection. Crop Journal, 6(6): 565-575.
Hojilla‐Evangelista, M.P., Selling, G.W., Hatfield, R. and Digman, M. 2017. Extraction, composition, and functional properties of dried ANR (Medicago sativa L.) leaf protein. Journal of the Science of Food and Agriculture, 97(3): 882-888.
Homolka, P., Koukolova, V., Podsednicek, M. and Hlavackova, A., 2012. Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods. Czech Journal of Animal Science, 57 (10): 454-568.
ICAR. 2013. Nutritional Requirements of Sheep, Goat and Rabbit, 2nd Ed., Indian Council of Agricultural Research, New Delhi, pp.7-8.
Koschuh, W., Povoden, G., Thang, V.H., Kromus, S., Kulbe, K.D., Novalin, S. and Krotscheck, C., 2004. Production of leaf protein concentrate from ryegrass (Lolium perenne x multiflorum) and alfalfa (Medicago Sativa) Comparison between heat coagulation/centrifugation and ultrafiltration. Desalination, 163 (1-3): 253-259.
Lei, Y., Hannoufa, A. and Yu, P. 2017. The use of gene modification and advanced molecular structure analyses towards improving Alfalfa forage. International Journal of Molecular Science, 18 (2): 298.
Lu, C.D. and Jorgensen, N.A., 1997. LNR Protein Concentrate as a Less Ruminally Degradable Protein Source for High-Producing Dairy Cows. Journal of Agricultural and Marine Sciences, 2:31-35.
Lu, C.D., Jorgensen, N.A. and Satter, L.D., 1988. Site and extent of nutrient digestion in lactating dairy cows fed LNR protein concentrate or soybean meal. Journal of Dairy Science, 71 (3):697-704.
Madhekar, R.D. and Mungikar, A.M. 2009. Chemical composition of Alfalfa (Medicago sativa L.) leaf meal and leaf protein concentrate. Journal of Phytological Research, 22: 95-98.
Menke, K.H. and Steingass, H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28: 7-55
NASEM, 2021. National Academies of Sciences, Engineering and Medicine, 2021. Nutrient Requirements of Dairy Cattle, 8th Ed. National Academy Press, Washington, DC, The National Academies Press. https://doi.org/10.17226/25806.
Orskov, E.R. and McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, 92 (2): 499-503.
Pavan B.L., V. Nagabhushana, G.U. Manju, K.S. Giridhar, N. Suma and B.U. Umesh. Effect of Feeding Sorghum Hay, Maize Stover and Areca Sheath Based Total Mixed Ration Feed Block (TMRfb) on Nutrient Intake, Digestibility and Cost Economics in Growing Lambs. Indian Journal of Animal Nutrition, 2024. 42 (1): 54-60.
Rani, K.J., Ally, K., Seethal, C.C., Surej, J.B. and Rajkumar, G. 2016. Effect of dietary incorporation of nutraceutical residue on dry matter intake and nutrient digestibility in crossbred calves. Journal of Veterinary and Animal Science, 47 (2): 78-81.
Samanta, A.K., Singh, K.K., Das, M.M., Maity, S.B. and Kundu, S.S., 2003. Effect of complete feed block on nutrient utilisation and rumen fermentation in Barbari goats. Small Ruminant Research, 48 (2): 95-102.
Singh, C.K., Robinson P.H. and Mc Niven, M.A. 1995. Evaluation of raw and roasted lupin seeds as protein supplement for lactating cows. Animal Feed Science and Technology, 52: 63-76.
Singh, P.K., Chandramoni, Kumar, K. and Kumar, S., 2016. Effect of feeding wheat and rice straw based complete feed blocks on nutrients utilization, blood biochemical and growth performance in crossbred calves. Indian Journal of Animal Science, 86 (7): 771-776.
Van Soest, P.V., Robertson, J.B. and Lewis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74 (10): 3583-3597.
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