Zinc Oxide Nanoparticles: A Comprehensive Study of Its Effects on Growth Performance and Intestinal Barrier Functions in Broiler Chickens
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Authors
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V. Mozhiarasi
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R. Karunakaran
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P. Raja
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L. Radhakrishnan
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N.Pazhanivel
Keywords:
Broiler, Growth and Intestinal Health, ZnONPsAbstract
Nano-trace elements have gained significant attention as a promising, emerging technology in the animal feed industry in recent years. Their minute size allows for enhanced absorption and efficiency compared to conventional zinc counterparts. The present study aimed to investigate the effects of Zinc oxide nanoparticles (ZnONPs) on the growth and intestinal barrier function of broiler chicks. A total of 150 one-day-old straight-run broiler chicks were randomly allotted to 5 dietary treatment groups. The chicks were fed with a basal diet supplemented with 80 mg/kg zinc oxide (control group) or 60 mg/kg zinc methionine, or 60, 40, or 20 mg/kg ZnONPs, respectively, for 35 days., Improved feed conversion ratio (FCR) was recorded in birds fed 20 and 40 mg/kg ZnONPs, among all the treatment groups. The different treatment groups did not affect the feed intake. Compared with the control group, the villus length (VL) and crypt depth (CD) in the duodenum and jejunum were increased in the 20 mg/kg ZnONPs group. In the ileum, CD was significantly higher in all studied doses of ZnONPs. Villi length to crypt depth ratio (V/C) was higher in ZnONPs groups in the duodenum, at 20 mg/kg ZnONPs in the ileum and in the jejunum. Dietary inclusion of ZnONPs showed no significant effects on mRNA expression of the Zona Occludens-1 (ZO-1) gene, while 20 mg/kg ZnONPs significantly upregulated the mucin-2 and claudin-3 genes in the ileal mucosa. The findings of the present study suggest that ZnONPs at a lower dose (20 mg/kg) promote growth and intestinal health.
References
Abd El-Ghany, W.A., Shaalan, M. and Salem, H. M. 2021. Nanoparticles applications in poultry production: an updated review. World's Poultry Science Journal. 77(4): 1001-1025. https://doi.org/10.1080/00439339.2021.1960235.
Ali, S., Masood, S., Zaneb, H., Faseeth-ur-Rehman, H., Masood, S., Khan, M., Tahir, S.K. and Rehman, H. 2017. Supplementation of zinc oxide nanoparticles has beneficial effects on intestinal morphology in broiler chicken. Pakistan Veterinary Journal. 37(3): 335-339.
AOAC, 2012. Official Methods of Analysis. 19th ed. Association of Official Analytical Chemist. Int., Arlington, VA.
Asheer, M., Manwar, S. J., Gole, M. A., Sirsat, S., Wade, M. R., Khose, K. K. and Sajid Ali, S. 2018.Effect of dietary nano zinc oxide supplementation on performance and zinc bioavailability in broilers. Indian Journal of Poultry Science. 53(1): 70-75.
Bami, M.K., Afsharmanesh, M., Salarmoini, M. and Tavakoli, H. 2018. “Effect of zinc oxide nanoparticles and Bacillus coagulans as probiotic on growth, histomorphology of intestine, and immune parameters in broiler chickens. Comparative Clinical Pathology. 27: 399-406.
Barzegar M., Zaghari, M., Zhandi, M. and Sadeghi, M. 2021. Effects of zinc dosage and particle size on gut morphology, tight junctions and TNF‐α expression in broiler breeder hens. Journal of Animal Physiology and Animal Nutrition. 106(4): 772-782. https://doi.org/10.1111/jpn.13638.
BIS, 2007. Bureau of Indian Standards, Indian Standards for Poultry Feeds - Specifications. 5th ed. New Delhi.
Bobíková K., Levkuta, M., Husáková, E., Levkutová, M., Kissová, V., Ivanišinová, O. 2016. Effect of glycine-zinc complex on mucin and iga expression, secretory iga concentration and lengths of intestinal villi in chickens. Journal of Comparative Pathology. 154:81. http://dx.doi.org/10.1016%2Fj.jcpa.2015.10.058.
Bratz, K., Golz, G., Riedel, C. 2013. Inhibitory effects of high dosage zinc oxide dietary supplementation on Camphylobacter coli excretion in weaned piglets. Journal of Applied Microbiology. 115 (5): 1194-1202. https://doi.org/10.1111/jam.12307.
Duncan D.B. 1955. Multiple range and multiple F tests. Biometrics. 11: 1-42. https://doi.org/10.2307/3001478.
El-Katcha, M., Soltan, M.A. and El-Badry, M. 2017. Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken. Alexandria Journal for Veterinary Sciences. 55(2): 129-145. http://dx.doi.org/10.5455/ajvs.266925.
Fathi, M., Abdullah, M., Haydari and Tanha, T. 2016. Effects of zinc oxide nanoparticles on antioxidant status, serum enzymes activities, biochemical parameters and performance in broiler chickens. Journal of Livestock Science and Technology. 4(2): 7-13. https://doi.org/10.22103/jlst.2016.1509.
Fatholahi A., Khalaji, S., Hosseini, F. and Abbasi, M. 2021. “Nano-Bio zinc synthesized by Bacillus subtilis modulates broiler performance, intestinal morphology and expression of tight junction's proteins. Livestock Science. 251:104660. https://doi.org/10.1016/j.livsci.2021.104660.
Fawaz R., Ibrahim, R.S., Ali, N.M., Mahmoud, U.T. and Amen, O. 2021. Effect of Dietary Zinc Oxide Nanoparticles on Growth Performance and Clostridium perfringens Infection in Broiler Chickens. Bulgarian Journal of Veterinary Medicine 2021: 1-14. https://doi.org/10.15547/bjvm.2021-0073.
Hafez A., Hegazi, S.M., Bakr, A.A. and Shishtawy, H.E. 2017. Effect of zinc oxide nanoparticles on growth performance and absorptive capacity of the intestinal villi in broiler chickens. Life Science Journal. 14: 67-72. https://doi.org/10.1007/s11356-019-04344-6.
Hafez, A., Nassef, E., Fahmy, M., Elsabagh, M., Bakr, A. and Hegazi, E. 2020. Impact of dietary nano-zinc oxide on immune response and antioxidant defense of broiler chickens. Environmental Science and Pollution Research. 27: 19108-19114. https://doi.org/10.1007/s11356-019-04344-6.
Ibrahim, D., Ali, H.A., and El-Mandrawy, S.A. 2017. Effects of different zinc sources on performance, bio distribution of minerals and expression of genes related to metabolism of broiler chickens. Zagazig Veterinary Journal. 45(3): 292-304. https://doi.org/10.21608/zvjz.2017.7954.
Jose N, Elangovan A.V., Awachat, V.B., Shet, D., Ghosh, J. and David, C.G. 2018. Response of in ovo administration of zinc on egg hatchability and immune response of commercial broiler chicken. Journal of Animal Physiology and Animal Nutrition. 102(2): 591-595. https://doi.org/10.1111/jpn.12777.
Lail, N.U., Sattar, A., Omer, M.O., Hafeez, M.A., Khalid, A.R., Mahmood, S., Shabbir, M.A., Ahmed, W., Aleem, M.T., Alouffi, A. and Almutairi, M.M. 2023. Biosynthesis and characterization of zinc oxide nanoparticles using Nigella sativa against coccidiosis in commercial poultry. Scientific Reports. 13(1): 6568. https://doi.org/10.1038/s41598-023-33416-4.
Lei X.J., Ru, Y.J. and Zhang, H.F. 2014. Effect of Bacillus amyloliquefaciens based direct-fed microbials and antibiotic on performance, nutrient digestibility, cecal microflora, and intestinal morphology in broiler chickens. Journal of Applied Poultry Research 23(3): 486–493. https://doi.org/10.3382/japr.2014-00965.
Livak, K.J. and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods. 25(4): 402-408. https://doi.org/10.1006/meth.2001.1262.
Mahmoud, U.T., Abdel-Mohsein, H.S., Mahmoud, M.A., Amen, O.A., Hassan, R.I., Abd-El-Malek, A.M., Rageb, S.M., Waly, H.S., Othman, A.A. and Osman, M.A. 2020. Effect of zinc oxide nanoparticles on broilers’ performance and health status. Tropical Animal Health and Production. 52(4): 2043-2054. https://doi.org/10.1007/s11250-020-02229-2.
McDonald, P., Edwards, R.A., and Greenhalagh, J.F.D. 2010. Animal Nutrition. New York.
Mozhiarasi, V., Karunakaran, R., Raja, P. and Radhakrishnan, L. 2023. Effects of Zinc Oxide Nanoparticles Supplementation on Growth Performance, Meat Quality and Serum Biochemical Parameters in Broiler Chicks. Biological Trace Element Research. 1-16. https://doi.org/10.1007/s12011-023-03759-0.
National Research Council, Nutrient requirements of dairy cattle: 2001. National Academies Press.
Qu, J., Zuo, X., Xu, Q., Li, M., Zou, L., Tao, R., Liu, X., Wang, X., Wang, J., Wen, L. and Li, R. 2023. Effect of Two Particle Sizes of Nano Zinc Oxide on Growth Performance, Immune Function, Digestive Tract Morphology, and Intestinal Microbiota Composition in Broilers. Animals. 13(9): 1454. https://doi.org/10.3390/ani13091454.
Rio, D.C., Ares, M., Hannon, G.J. and Nilsen, T.W. 2010. Purification of RNA using TRIzol (TRI reagent). Cold Spring Harbor Protocols. 2010(6). https://doi:10.1101/pdb.prot5439.
Tsirtsikos P, Fegeros, K., Kominakis, A., Balaskas C. and Mountzouris, K. 2012. Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers. Animal. 6:1049– 1057. https://doi.org/10.1017/S1751731111002680.
Xie, Z., Zhao, Q., Wang, H., Wen, L., Li, W., Zhang, X., Lin, W., Li, H., Xie Q., and Wang, Y. 2020. Effects of antibacterial peptide combinations on growth performance, intestinal health, and immune function of broiler chickens. Poultry science. 99(12): 6481-6492.
Uni ZEHAVA, Ganot SAHAR and Sklan DAVID (1998). Post hatch development of mucosal function in the broiler small intestine. Poultry Science. 77(1): 75-82. https://doi.org/10.1093/ps/77.1.75.
Yusof, H.M., Mohamad, R., Zaidan, U.H., Arshad M.A. and Samsudin, A.A. 2023. Effects of dietary zinc oxide nanoparticles supplementation on broiler growth performance, zinc retention, liver health status, and gastrointestinal microbial load. Journal of Trace Elements and Minerals. 4: 100072. https://doi.org/10.1016/j.jtemin.2023.100072.
Zhang, J., Yu, C., Li, Z., Li, J., Chen, Y., Wang, T. and Wang, C. 2022. Effects of zinc oxide nanoparticles on growth, intestinal barrier, oxidative status and mineral deposition in 21-day-old broiler chicks. Biological Trace Element Research. 200(4): 1826-1834. https://doi.org/10.1007/s12011-021-02771-6.
Zhao, C.Y., Tan, S.X., Xiao, X.Y., Qiu, X.S., Pan, J.Q. and Tang, Z.X. 2014. Effects of dietary zinc oxide nanoparticles on growth performance and antioxidative status in broilers. Biological Trace Element Research. 160: 361-367. https://doi.org/10.1007/s12011-014-0052-2.
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