Molecular characterization of Trichogramma spp. in sugarcane (Saccharum officinarum) ecosystem in Punjab

JASREET KAUR; VIKAS JINDAL; KAMALDEEP SINGH SANGHA

doi:10.56093/ijas.v93i6.101542

Authors

JASREET KAUR Punjab Agricultural University, Ludhiana, Punjab 141 001, India
VIKAS JINDAL Punjab Agricultural University, Ludhiana, Punjab 141 001, India
KAMALDEEP SINGH SANGHA Punjab Agricultural University, Ludhiana, Punjab 141 001, India

https://doi.org/10.56093/ijas.v93i6.101542

Keywords:

Biological control, DNA barcoding, Population, Species, Sugarcane

Abstract

Trichogramma chilonis (Ishii) is of great importance to biological control of many harmful lepidopteran pests of crops. There is a need to establish the species composition in the fields for differentiation among the species/strains in order to enhance efficiency of their practical application. This study aimed to catalogue the species of Trichogramma available in sugarcane in Punjab. The populations were collected from different districts of Punjab, viz. Gurdaspur, Shaheed Bhagat Singh Nagar, Patiala, Amritsar and Fazilka during 2017–18. The mtCOI gene of Trichogramma was amplified and sequenced for identification of species and assessing the genetic diversity. Nucleotide sequences of Trichogramma populations collected from different sugarcane growing districts of Punjab showed 99% homology with T. chilonis. Multiple alignments of mtCOI sequences showed three branches with Punjab populations similar to New Delhi, Bengaluru and Pakistan. This study confirms the successful establishment of T. chilonis in sugarcane ecosystem in Punjab.

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References

Chakraborty K. 2011. Report on egg parasitoids of Scirpophaga incertulas from Hemtabad, West Bengal. Annals of Plant Protection Sciences 19: 213–14.

Folmer O, Black M, Hoeh W, Lutz R and Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–99.

Kaur R and Brar K S. 2008. Evaluation of different doses of Trichogramma species for the management of leaf folder and stem borer on basmati rice. Journal of Biological Control 22: 131–35.

Monaghan M T, Balke M, Gregory T R and Vogler A P. 2005. DNA-based species delineation in tropical beetles using mitochondrial and nuclear markers. Philosophical Transactions of the Royal Society of London Biological Sciences 360: 1925–33. DOI: https://doi.org/10.1098/rstb.2005.1724

Nagaraja H and Mohanraj P. 2010. Three new species of Trichogramma (Hymenoptera: Trichogrammatidae) from southern India. Journal of Biological Control 24: 203–09.

Pinto J D, Stouthamer R, Platner G R and Oatman E R. 1991. Variation in reproductive compatibility in Trichogramma and its taxonomic significance (Hymenoptera: Trichogrammatidae). Annals of the Entomological Society of America 84: 37–46. DOI: https://doi.org/10.1093/aesa/84.1.37

Querino R B and Zucchi R A. 2002. Intraspecific variation in Trichogramma bruni Nagaraja, 1983 (Hymenoptera: Trichogrammatidae) associated with different hosts. Brazilian Journal of Biology 62: 665–69. DOI: https://doi.org/10.1590/S1519-69842002000400015

Rijesh K, Puja R K, Hegde R, Joseph A, Jalali S K and Kush A. 2012. Genetic diversity estimates in Trichogramma populations from sugarcane cropping systems in India Karnataka Journal of Agricultural Sciences 25: 68–71.

Singh S, Shenhmar M, Brar K S and Jalali S K. 2007. Evaluation of different strains of Trichogramma chilonis Ishii for the suppression of sugarcane early shoot borer, Chilo infuscatellus Snellen. Journal of Biological Control Control 21: 247–53.

Sharma S, Shera P S, Kaur R and Sangha K S .2016. Standardization of egg dosages for mass production of Corcyra cephalonica (Stainton). Phytoparasitica 44: 459–64. DOI: https://doi.org/10.1007/s12600-016-0542-1

Shera P S, Sharma S, Jindal J, Bons M, Singh G, Kaul A, Kaur R and Sangha K S. 2017. On-farm impact of egg parasitoid, Trichogramma chilonis against maize stem borer, Chilo partellus in Punjab. Indian Journal of Agricultural Sciences 87: 1412–15. DOI: https://doi.org/10.56093/ijas.v87i10.75062

Yousuf M, Khan S, Ikram M and Singh K P .2016. Diversity of biological control agents Trichogramma from Punjab (India) and its importance in forestry and agroforestry systems. Journal of Environment and Bio-Sciences 30: 541–47.

Yu-Di L, Mao-Lin H and Song K. 2017. Molecular identification of Trichogramma species from South and South-East Asia and natural Wolbachia infection. Entomologica Fennica 28: 57–66. DOI: https://doi.org/10.33338/ef.84676