Biocontrol of Fusarium wilt of tomato by nano-extract of Trichoderma harzianum

Mahya Rahimizadeh; Mehdi Sadravi

doi:10.56093/ijas.v90i6.104754

Authors

Mahya Rahimizadeh Yasouj University, Yasouj 75918-74831, Iran
Mehdi Sadravi Yasouj University, Yasouj 75918-74831, Iran

https://doi.org/10.56093/ijas.v90i6.104754

Keywords:

Fusarium wilt, Nano, Silver, Tomato, Trichoderma

Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici is an important disease of tomato worldwide. The pathogen was isolated and purified from infected plants in tomato-growing greenhouses in southwest Iran during 2017. The impact of colony’s extract of four Trichoderma harzianum isolates were tested on colony growth of the pathogen in-vitro. Premium isolate colony's extract of T. harzianum was converted to nano-product by mixing with silver-nitrate. The formation of nano-particles in this product was evaluated by dynamic light scattering, and scanning electron microscopy. The impact of nano-product was tested on colony growth of the pathogen and its EC₅₀ was determined. Then the impact of this product and silver-nitrate was tested on the disease severity in two tomato varieties in-vivo. Colony’s extract of all four isolates of T. harzianum reduced the colony growth of the pathogen, but the effect of an isolate’s extract of T.harzianum 39 was more. Produced nano-extract had EC50441.9ppm for the pathogen. It also caused a significant reduction of the disease severity in-vivo, and its effect was more than silvernitrate alone. Potential of T. harzianum nano-extract for biological control of the disease is being reported for the first time as per our knowledge.

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References

Agrios G N. 2005. Plant Pathology. Academic Press Inc., New York, USA.

Akrami M and Yousefi Z. 2015. Biological control of Fusarium wilt of tomato (Solanum lycopersicum) by Trichoderma spp. as antagonist fungi. Biological Forum – An International Journal 7(1): 887-92

Amini J, Kazemi M, Abdollahzadeh J and Darvishnia M. 2014. Identification of Fusarium species associated with root and crown rot of tomato in Marvdasht. Iranian Plant Protection Science 44:71-80.

Amutha C and Darwin ChristdhasHenry L. 2017. Survey and severity of tomato wilt disease incited by Fusarium oxysporum f.sp. lycopersici (Sacc.) in different districts of Tamil Nadu. International Journal of Recent Scientific Research 8(12): 22702-04.

Banu A and Rathod V. 2011. Synthesis and characterization of silver nanoparticles by Rhizopus stolonifer. International Journal of Biomedical and Advance Research 2: 148-58. DOI: https://doi.org/10.7439/ijbar.v2i5.30

Bawa I. 2016. Management strategies of Fusarium wilt disease of tomato incited by Fusarium oxysporum f. sp. lycopersici: A review. International Journal of Advanced Academic Research 2: 32–42.

Devi T P, Kulanthaivel S, Kamil D, Borah J L, Prabhakaran N and Srinivasa N. 2013. Biosynthesis of silver nano-particles from Trichoderma species. Indian Journal of Experimental Biology 51: 543-47.

Ershad D. 2009. Fungi of Iran. Iranian Research Institute of Plant Protection, Tehran, Iran.

Guilger M, Pasquoto-Stigliani T, Bilesky-Jose N, Grillo R, Abhilash P C, Fraceto L F, and Lima R. 2017. Biogenic silver nanoparticles based on Trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity. Scientific Reports 7: 44421. Doi: 10.1038/srep44421 DOI: https://doi.org/10.1038/srep44421

Khlebtsov B N and Khlebtsov N G. 2011. On the measurement of gold nanoparticle sizes by the dynamic light scattering method. Colloid Journal 73: 118–27. DOI: https://doi.org/10.1134/S1061933X11010078

Kim S W, Jung J H, Lamsal K K, Min Y S and Lee Y S. 2012. Antifungal effects of silver nanoparticles (AgNPs) against various plant pathogenic fungi. Mycobiology 40: 53-58. DOI: https://doi.org/10.5941/MYCO.2012.40.1.053

Leslie J F and Summerell B A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Ltd. Oxford, UK. DOI: https://doi.org/10.1002/9780470278376

Lim S F, Riehn R, Ryu W S, Khanarian N, Tung C-k, Tank D and Austin R H. 2005. In vivo and scanning electron microscopy imaging of up converting nanophosphors in Caenorhabditis elegans. Nano Letters 6:169–74. DOI: https://doi.org/10.1021/nl0519175

Mwangi M W, Monda E O, Okoth S A and Jefwa J M. 2011. Inoculation of tomato seedlings with Trichoderma harzianum and Arbuscular Mycorrhizal Fungi and their effect on growth and control of wilt in tomato seedlings. Brazilian Journal of Microbiology 42(2): http://dx.doi.org/10.1590/S1517- 83822011000200015 DOI: https://doi.org/10.1590/S1517-83822011000200015

Naveen H K S, Kumar G, Karthik L and Rao B K V. 2010. Exteracellular biosynthesis of silver nanoparticles using the filamentous fungus Penicillium sp. Archives of Applied Science Research 2:161-67.

Rai M, Yadav A and Gade A. 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances 27:76–83. DOI: https://doi.org/10.1016/j.biotechadv.2008.09.002

Reino J L, Guerriero R F, Hernàndez-Galà R and Collado I G. 2008. Secondary metabolites from species of the biocontrol agent Trichoderma. Phytochemistry Reviews 7(1): 89–123. DOI: https://doi.org/10.1007/s11101-006-9032-2

Reis A, Costa H, Boiteux L S and Lopes C A. 2005. First report of Fusarium oxysporum f. sp. lycopersici race 3 on tomato in Brazil. Fitopatologia Brasileira 30: 426–28. DOI: https://doi.org/10.1590/S0100-41582005000400017

Roy S, Mukherjee T, Chakraborty S and Das T. 2013. Biosynthesis, characterisation and antifungal activity of silver nanoparticles synthesized by the fungus Aspergillus foetidus mtcc8876. Digest Journal of Nanomaterials and Biostructures 8: 197-205.

Shelar G B and Chavan A M. 2015. Myco-synthesis of silver nanoparticles from Trichoderma harzianum and its impact on germination status of oil seed. Biolife Journal 3: 109-13.

Sibounnavong P, Keoudone C, Soytong K, Divina C C and Kalaw S P. 2010. A new mycofungicide from Emericella nidulans against tomato wilt caused by Fusarium oxysporum f. sp. lycopersici in vivo. Journal of Agricultural Technology 6: 19-30.

Vinale F, Marra R, Scala F, Ghisalberti E L, Lorito M and Sivasithamparam K. 2006. Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Letters in Applied Microbiology 43: 143–48. DOI: https://doi.org/10.1111/j.1472-765X.2006.01939.x

Veerasamy R, Xin T Z, Gunasagaran S, Xiang T FW, Chou-Yang E F, Jeyakumar N and Dhanaraj S A. 2011. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society 15: 113–20. DOI: https://doi.org/10.1016/j.jscs.2010.06.004