Effect of scarification treatments on seed germination of guava (Psidium guajava)

SOURABH SOURABH; PREETI PREETI; JEET RAM SHARMA; AXAY BHUKAR

doi:10.56093/ijas.v90i7.105612

Authors

SOURABH SOURABH CCS Haryana Agricultural University, Hisar, Haryana 125 004, India
PREETI PREETI CCS Haryana Agricultural University, Hisar, Haryana 125 004, India
JEET RAM SHARMA CCS Haryana Agricultural University, Hisar, Haryana 125 004, India
AXAY BHUKAR CCS Haryana Agricultural University, Hisar, Haryana 125 004, India

https://doi.org/10.56093/ijas.v90i7.105612

Keywords:

Dormancy, Guava, Hot water treatment, Scarification, Sulphuric acid

Abstract

The present study was carried out to investigate the effect of pre-sowing and scarification treatments on seed germination and seedling growth of guava (Psidium guajava L.) in the laboratories of the Department of Seed Science and Technology, CCS HAU, Hisar during the year 2015. There were three scarification methods with different treatments under study, i.e. water soaking (for 24, 48 and 72 hr), hot water soaking (at 70, 80, 90 and 100ÂºC) and sulphuric acid soaking (at 10, 20, 30, 40 and 50% concentration). Duration for hot water soaking and sulphuric acid soaking was kept as quick dip (5 seconds), 1 min. and 3 min. The study revealed that pre soaking the seeds in hot water and sulphuric acid greatly increased germination, especially soaking of seeds in 20% sulphuric acid solution for 3 min., which showed up to 72% germination. Parameters like germination%, mean germination time, seedling length and seedling vigour were positively affected by all the scarification treatments. Seed viability decreased but the standard germination efficiently increased due to scarification treatments. Hot water soaking at 80ÂºC temperature for 1 min. improved germination and resulted in maximum seedling length (6.03 cm) in laboratory. Seeds treated with higher sulphuric acid concentrations or soaked in hot water at higher temperature showed poor germination as well as poor vigour. Seeds without any scarification treatment showed poor germination.

Downloads

Download data is not yet available.

References

Abdul-Baki A A and Anderson J D. 1973. Vigour determination in soybean seed by multiple criteria. Crop Science 13: 630–632. DOI: https://doi.org/10.2135/cropsci1973.0011183X001300060013x

Ali N, Mulwa R M S, Morton M A and Skirvin R M. 2007. Radical disinfestation protocol eliminates in vitro contamination in guava (Psidium guajava L.) seeds. Plant Cell Tissue Organ Culture 91: 295–298. DOI: https://doi.org/10.1007/s11240-007-9283-7

Baskin C C. 2003. Breaking physical dormancy in seeds – focusing on the lens. New Phytologist 158: 227–238. DOI: https://doi.org/10.1046/j.1469-8137.2003.00751.x

Brijwal M, Kumar R and Mishra D S. 2013. Effect of pre-sowing treatments on seed germination of guava (Psidium guajava L.) under Tarai region of Uttarakhand. Progressive Horticulture 45(1): 154–159.

David F H and Midcap J T. 2007. Seed Propagation of Woody Ornamentals. University Press, University of Florida, Florida, USA.

Gomez K A and Gomez A A. 1984. Statistical Procedures for Agricultural Research, 2nd edn, p 680. John Wiley and Sons, New York.

Harb A M. 2013. Reserve mobilization, total sugars and proteins in germinating seeds of durum wheat (Triticum durum Desf.) under water deficit after short period of imbibition. Jordan Journal of Biological Sciences 6: 67–72. DOI: https://doi.org/10.12816/0000261

ISTA. 2001. Rules amendments 2001. Seed Science and Technology 29(supplement): 13–33.

Maslin B R and McDonald M W. 2004. Acacia search: evaluation of Acacia as a woody crop option for southern Australia. Rural Industries Research and Development Corporation, Barton, ACT.

Moradi Dezfuli P, Sharif-zadeh F and Janmohammadi M. 2008. Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). ARPN Journal of Agricultural and Biological Science 3(3): 22–25.

Musara, C, Chitamba J and Nhuvira C. 2015. Evaluation of different seed dormancy breaking techniques on okra (Abelmoschus esculentus L.) seed germination. African Journal of Agricultural Research 10(17): 1952–1956. DOI: https://doi.org/10.5897/AJAR2014.9181

Nayak G and Sen S K. 1999. Effect of growth regulators, acid and mechanical scarification on germination of bael (Aegle marmelos C.). Environment and Ecology 17(3): 768–769

Nayaka G. 2006. ‘Propagation studies in aonla (Phyllanthus emblica L.)’. MSc thesis, University of Agricultural Science, Dharwad, Karnataka, India.

Ostle B and Mensing R W. 1975. Statistics in Research. Basic Concepts and Techniques for Research Workers, 3rd edition. Ames: IOWA State University Press, USA.

Pandey D and Singh G. 2000. Effect of seed pretreatment on promotion of germination in guava. Annals of Agricultural Research 21: 279–281.

Saxena M and Gandhi C P. 2017. Indian Horticulture Database, National Horticulture Board, Ministry of Agriculture, Government of India, India.

Sedbrook J. 2006. Gardening and Horticulture. Denver County Cooperative Extension, Colorado, USA.

Srinivasan K and Saxena S. 2007. Removal of tenacious glumes enhances the seed germination and seedling vigour in eight Aegilops species for efficient conservation in genebank. Indian Journal of Agricultural Sciences 83(4): 437–40.

Teketay D. 1998. Germination of Acacia origena, A. pilispina and Pterolobium stellatum in response to different pre-sowing seed treatments, temperature and light. Journal of Arid Environment 38: 551–560. DOI: https://doi.org/10.1006/jare.1997.0332