Ex vitro hardening of sugarcane (Saccharum Species Hybrid) clones for rapid multiplication

MENIARI TAKU; T E NAGARAJA; H C LOHITHASWA; K V SHIVAKUMAR; SURESH YADAV

doi:10.56093/ijas.v90i12.110344

Authors

MENIARI TAKU Ph D Scholar, Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India
T E NAGARAJA Professor (Plant Breeding) and Head, All India Coordinated Research Project on Pigeon Pea, GKVK Campus, UAS, Bengaluru, Karnataka
H C LOHITHASWA Professor and Head (Plant Breeding), College of Agriculture, V C Farm, Mandya, UAS, Bengaluru, Karnataka;
K V SHIVAKUMAR Professor (Crop Physiology), College of Agriculture, V C Farm, Mandya, UAS, Bengaluru, Karnataka
SURESH YADAV Ph D Scholar, Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India

https://doi.org/10.56093/ijas.v90i12.110344

Keywords:

Hardening, Humidity, Light Intensity, Micropropagation, Sugarcane, Temperature

Abstract

The hardening of in vitro raised plantlets is essential for better survival and successful establishment. In the present study an attempt was made to find out the best ex vitro condition (single shade net and double shade net) for hardening of five different clones of sugarcane. The effect of temperature, light intensity and humidity on hardening of elite sugarcane genotypes were evaluated under two different hardening conditions. The results obtained in the present investigation concluded that both the ex vitro acclimatization conditions showed almost similar effect on number of leaves per plant. However, for chlorophyll content (mg/g), root characters, viz. number of roots, root length (cm), root volume (cm3) and survival ability, double shade net was found to be optimum for all the genotypes, viz. CoVC 09-61-02, CoVC 07-06-05, CoVC 09-61-07, CoC 671 and CoVC 10-38-07. Whereas, CoVC 07-06-05 and CoC 671 produced optimum shoot length under single shade net. Hence, it is clear that the double shade net served as better hardening condition as revealed from better survival and development of appropriate morphological characters of all the sugarcane clones.

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References

Arun K T and Baishnab C T. 1998. Temperature stress induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat. Plant Physiology 117: 851-858. DOI: https://doi.org/10.1104/pp.117.3.851

Bhojwani S S and Razdan M K. 1996. Plant tissue culture: theory and practice. Elsevier 5: 766. DOI: https://doi.org/10.1016/S0928-3420(96)80002-4

Chandra S, Rajib B, Vijay K and Ramesh C. 2010. Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnology Letters 32: 1199-1205. DOI: https://doi.org/10.1007/s10529-010-0290-0

Dave A, Bilochi G and Purohit S D. 2003. Scaling-up production and field performance of micropropagated medicinal herb ‘Safed Musli’ (Chlorophytum borivilianum). In Vitro Cell Developmental Biology 39(4): 419-42. DOI: https://doi.org/10.1079/IVP2003431

Dhawan V and Bhojwani S S. 1986. Micropropagation in crop plants. Plant Research 7: 1-75.

Deb C R and Imchen T. 2010. An efficient in vitro hardening technique of tissue culture raised plants. Biotechnology 9: 79-83. DOI: https://doi.org/10.3923/biotech.2010.79.83

Debergh P. 1988. Micropropagation of woody species state of the art on in vitro aspects. Acta Horticulturae 227: 287-295. DOI: https://doi.org/10.17660/ActaHortic.1988.227.53

Jo E A, Tewari R K, Hahn E J and Paek K Y. 2007. Effect of photoperiod and light intensity on in vitro propagation of Alocasia amazonica. Plant Biotechnology 2: 207-212. DOI: https://doi.org/10.1007/s11816-008-0063-6

Meziani R, Fatima J, Mouaad A M, Mohamed A, Mustapha A C, Jamal E F and Chakib A. 2015. Effects of plant growth regulators and light intensity on the micropropagation of date palm (Phoenix dactylifera L.) cv. Mejhoul. J. Crop Sci. Biotech. 18(5): 325-331. DOI: https://doi.org/10.1007/s12892-015-0062-4

Poole R T and Conover C A. 1983. Establishment and growth of in vitro cultured Dieffenbachia. Hort. Sci. 18: 185-187. DOI: https://doi.org/10.21273/HORTSCI.18.2.185

Rani A and S Kumar. 2017. Tissue culture as a plant production technique for medicinal plants: a review. International Conference on Innovative Research in Science Technology and Management, 22nd -23rd January 2017.

Richardson S D. 1953. Studies of root growth in Acer saccharinum L, I. The relation betweenroot growth and photosynthesis. Proc. Kon. Nederl. Akad. Wetensch. Amsterdam. C56, pp 185-193.

Ritchie G A, Short K C and Davey M R. 1991. In vitro acclimatization of chrysanthemum and sugar beet plantlets by treatment with Paclobutrazol and exposure to reduced humidity. Journal Experimental Botany 42(12):1557-1563. DOI: https://doi.org/10.1093/jxb/42.12.1557

Scaranari C, Paulo A, Martins L and Mazzafera P S. 2009. Periods of acclimatization of micropropagated banana plantlets cv. Grande naine. Sci. Agric. 66(3): 331-33. DOI: https://doi.org/10.1590/S0103-90162009000300008

Short K C, Warburton J and Robert A V. 1987. In vitro hardening of cultured cauliflower and chrysanthemum plantlets to humidity. Acta Horticulture 212: 329-334. DOI: https://doi.org/10.17660/ActaHortic.1987.212.50

Sutter E. 1984. Chemical composition of epicuticular wax in cabbage plants grown in vitro. Canada J. Bot 62: 74-77. DOI: https://doi.org/10.1139/b84-012

Wassink E C and Richardson S D. 1951. Observations on the connection between root growth and shoot illumination in first-year seedlings of Acer pseudoplatanus L. and Quercus borealis maxima (Marsh) Ashe. Proc. Kon. Nederl. Akad. Wetensch. Amsterdam.C 54, pp 503-510.

Yadav S, Nagaraja T E, Lohithaswa H C and Shivakumar K V. 2019. Effect of temperature, humidity and light intensity on micropropagated sugarcane (Saccharum Species Hybrid) genotypes. Sugar Tech 22: 226-231. DOI: https://doi.org/10.1007/s12355-019-00779-w