Evaluation of plant beneficial microbes for bio-hardening of in-vitro raised pomegranate saplings


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Authors

  • N V SINGH ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • N N KARWA ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • S B BIRAJDAR ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • SHILPA PARASHURAM ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • P G PATIL ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • K D BABU ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • D M MUNDEWADIKAR ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • VIPUL SANGNURE ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • V A GARGADE ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India
  • JYOTSANA SHARMA ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255, India

https://doi.org/10.56093/ijas.v91i1.110906

Keywords:

Bio-hardening, Micro-propagation, Plant beneficial microbes, Pomegranate

Abstract

Five beneficial microbial agents either alone or in combinations were used to inoculate one year old in-vitro raised pomegranate plants of cv. Bhagawa for further utilization of these microbes as bio-hardening agents. A pot culture experiment was initiated comprising 15 treatments each having three replications. The growth, physiological and biochemical parameters of the inoculated and control plants were recorded at 90 days after inoculation. Microbes and microbial formulations were evaluated for their further utilization as bio-priming agents in in-vitro raised pomegranate plants. Plants inoculated with Aspergillus niger strain AN27 (Asp) + Penicillium pinophilum registered significant increase in plant height (225.00 cm) and plant spread E-W (198.33 cm) as compared to control. Asp + Pseudomonas fluorescens inoculated plants performed significantly better with respect to total chlorophyll (1.84 mg/g FW), SPAD value (41.23) and total phenol content (0.81 mg/g FW) as compared to non-inoculated plants. Plants inoculated with AMF (Glomus intraradices) + T. viridae and T. harzianum was at par with Asp + P. fluorescens in total chlorophyll content (1.71 mg/g FW) and SPAD value of leaves (36.10). Plants inoculated with AMF in combination with P. pinophilum (T10), P. fluorescens (T11) and T. viridae and T. harzianum (T12) registered higher photosynthesis as compared to control and many other treatments. Microbial treatments were found effective in improving most of the morphological, physiological and biochemical attributes of inoculated pomegranate plants.

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References

Aseri G K, Jain N, Panwar J, Rao A V and Meghwal P R. 2008. Biofertilizers improve plant growth, fruit yield, nutrition, metabolism and rhizosphere enzyme activities of pomegranate (Punica granatum L.) in Indian Thar Desert. Scientia Horticulturae 117(2): 130–5. DOI: https://doi.org/10.1016/j.scienta.2008.03.014

Barnes J D, Balaguer L, Maurigue E, Elvira S and Davison A W. 1992. A reappraisal of the use of DMSO for the extraction and determination of chlorophyll ‘a’ and ‘b’ in lichens and higher plants. Environmental and Experimental Botony 32(2): 87–99. DOI: https://doi.org/10.1016/0098-8472(92)90034-Y

Hazarika B N. 2003. Acclimatization of tissue-cultured plants. Current Science 85: 1704–12.

Krishna H, Singh S K and Patel V B. 2006. Screening of arbuscular mycorrhizal fungi for enhanced growth and survival of micropropagated grape (Vitis vinifera) plantlets. Indian Journal of Agricultural Sciences 76(5): 297–301.

Krishna H, Singh S K, Sharma R and Khawale R N. 2005. Biochemical changes in micropropagated grape (Vitis vinifera L.) plantlets due to arbuscular-mycorrhizal fungi (AMF) inoculation during ex vitro acclimatization. Scientia Horticulturae 106(4): 554–67. DOI: https://doi.org/10.1016/j.scienta.2005.05.009

Maity A, Pal R K, Chandra R and Singh N V. 2014. Penicillium pinophilum-a novel microorganism for nutrient management in pomegranate (Punica granatum L.). Scientia Horticulturae 169: 111–17 DOI: https://doi.org/10.1016/j.scienta.2014.02.001

Malik C P and Singh M B. 1980. Plant Enzymology and Histo- Enzymology, p 286. Kalyani Publishers, New Delhi.

Mathur N and Vyas A. 1999. Improved biomass production, nutrient uptake and establishment of in vitro raised Ziziphus mauritiana by VA mycorrhiza. Journal of Plant Physiology 155 (1): 129–32. DOI: https://doi.org/10.1016/S0176-1617(99)80153-9

Paula M A, Siqueira J O and Dobereiner J. 1993. Occurrence of vesicular arbuscular mycorrhizal fungi and diazptrophic bacteria associated with sweet potato. Revista Brasileira de Ciência do Solo 17: 349–56.

Puthur J T, Prasad K V S K, Sharmila P and Pardhasaradhi P. 1998. Vesicular arbuscular mycorrhizal fungi improves establishment of micropropagated Leucaena leucocephala plantlets. Plant Cell Tissue Organ Culture 53: 41–47. DOI: https://doi.org/10.1023/A:1006068026377

Reid C P P. 1979. Mycorrhiza and Water Stress: The Soil–root Interface. Academic Press, New York.

Rupnawar B S and Navale A M. 2000. Effect of VA-mycorrhizal inoculation on growth of pomegranate layers. Journal of Maharashtra Agricultural University 25(1): 44–6.

Schubert A, Mazitelli M, Ariusso O and Eynard I. 1990. Effect of vesicular-arbuscular mycorrhizal fungi and micropropagated grapevines: influence of endophyte strain, P fertilization and growth medium. Vitis 29: 5–13.

Sen B. 2000. Biological control: A success story. Indian Phytopathology 53(3): 243–9.

Sharma J, Chandra R, Babu, K D, Meshram D T, Maity A, Singh N V and Gaikwad N N. 2014. Pomegranate: cultivation, marketing and utilization. Technical Bulletin No. NRCP/2014/1, ICAR-NRCP, Solapur, India.

Singh N V, Singh S K, Singh A K, Meshram D T and Suroshe S S. 2012. Arbuscular Mycorrhizal Fungi (AMF) induced hardening of micropropagated pomegranate (Punica granatum L.) plantlets. Scientia Horticulturae 36: 122–27. DOI: https://doi.org/10.1016/j.scienta.2012.01.005

Singh N V, Chandra R, Meshram D T, Maity A and Pal R K. 2013. Pomegranate: planting material for better quality. Indian Horticulture 59(3): 3–6.

Singh N V, Sharma J, Chandra R, Babu K D, Shinde Y R, Mundewadikar D M and Pal R K. 2016. Bio-hardening of in-vitro raised plants of Bhagwa pomegranate (Punica granatum). Indian Journal of Agricultural Science 86: 132–36.

Singh N V, Shilpa P, Roopasowjanya P, Babu K D, Mundewadikar D M, Sangnure V, Sharma, J and Pal RK. 2020. Performance evaluation of plants raised through different propagation methods in pomegranate (Punica granatum). Indian Journal of Agricultural Sciences 90(4): 685–88.

Thimmaiah S H. 2006. Standard methods of biochemical analysis. Kalyani publishers, Ludhiana, India.

Weatherley P E. 1950. Studies in water relation of cotton plant I. Field measurement of water deficits in leaves. New Physiologist 49: 81–97. DOI: https://doi.org/10.1111/j.1469-8137.1950.tb05146.x

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2021-03-01

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2021-03-02

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SINGH, N. V., KARWA, N. N., BIRAJDAR, S. B., PARASHURAM, S., PATIL, P. G., BABU, K. D., MUNDEWADIKAR, D. M., SANGNURE, V., GARGADE, V. A., & SHARMA, J. (2021). Evaluation of plant beneficial microbes for bio-hardening of in-vitro raised pomegranate saplings. The Indian Journal of Agricultural Sciences, 91(1), 29–33. https://doi.org/10.56093/ijas.v91i1.110906
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