Embryo rescue and hybridity establishment in hybrid progenies derived from polyemebryonic Citrus species

A K DUBEY; ANKIT GUPTA; NIMISHA SHARMA; R M SHARMA

doi:10.56093/ijas.v91i2.111637

Authors

A K DUBEY ICAR-Indian Agricultural Research Institute, New Delhi
ANKIT GUPTA ICAR-Indian Agricultural Research Institute, New Delhi
NIMISHA SHARMA ICAR-Indian Agricultural Research Institute, New Delhi
R M SHARMA ICAR-Indian Agricultural Research Institute, New Delhi

https://doi.org/10.56093/ijas.v91i2.111637

Keywords:

Embryo rescue, Germination, Hybrid, Nucellar, Polyembryonic, SSR

Abstract

Due to highly polyemebryonic nature of Rangpur lime and sour orange, development of hybrid is difficult through conventional breeding. Therefore, the present study was carried out at the Division of Fruits & Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi during 2017-19 to improve hybrid seedling recovery among the crosses made between these species. Germination was induced in rescued immature embryos ages (90-100, 110- 120, 130-140, 150-160 and 170-180 days after pollination) on two culture media (MS medium supplemented with 500 mg/l malt extract +1.5 mg/l GA3 + 0.02 mg/l NAA (M1) and DKW medium added with 1.5 mg/l GA3 + 0.02 mg/l NAA (M2). Fruits harvested at younger stage (90-120 DAP) had higher number of functional seeds. Older embryos (>150 days old) germinated earlier on either medium. Embryos rescued at early stage (90-100 DAP), middle age embryos (130-140 DAP) and oldest embryos (170-180 DAP) germinated efficiently (88.85-100%) on M2 medium. The seedling obtained from 170-180 days old embryos grown on M2 medium produced longest primary root and tallest plantlets (7.52 and 4.48 cm) at 60 days after inoculation (DAI). SSR marker analysis revealed that GT03 loci exhibited the polymorphism between male and female parents, proving their ability to detect the hybrids of Rangpur lime (C. limonia) × sour orange (C. aurantium).

Downloads

Download data is not yet available.

References

Bar Joseph M, Marcus R and Lee R F 1989. The continuous challenge of citrus tristiza virus control. Annual Review of Phytopathology 27: 291–16. DOI: https://doi.org/10.1146/annurev.py.27.090189.001451

Carimi F, De Pasquale F and Puglia A M. 1998. In vitro rescue of zygotic embryos of sour orange Citrus aurantium L. and their detection based on RFLP analysis. Plant Breeding 117: 261–66. DOI: https://doi.org/10.1111/j.1439-0523.1998.tb01936.x

Doyle J J and Doyle J L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.

Driver J A and Kuniyuki A H. 1984. In vitro propagation of paradox walnut rootstock. HortScience 19: 507–9. DOI: https://doi.org/10.21273/HORTSCI.19.4.507

Ji W, Li Z Q, Zhou Q, Yao W K and Wang Y J. 2013. Breeding new seedless grape by means of in vitro embryo rescue. Genetics Molecular Research 12: 859–69. DOI: https://doi.org/10.4238/2013.March.26.1

Liu S M, Sykes S R and Clingeleffer P R. 2003. Improved in ovulo embryo culture for stenospermocarpic grapes (Vitis vinifera L.). Australian Journal of Agricultural Research, 54: 869–76. doi: 10.1071/AR03053. DOI: https://doi.org/10.1071/AR03053

Liu K, Goodman M, Muse S, Smith J S, Buckler E and Doebley J. 2003. Genetic structure and diversity among maize inbred lines as inferred from DNA microsatellites. Genetics 165: 2117–28. DOI: https://doi.org/10.1093/genetics/165.4.2117

Murashige T and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantaram 15: 473–97. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Murashige T and Tucker P H. 1969. Growth factor requirements of citrus tissue culture. Proceeding of 1st International Citrus Symposium 3: 1155–61.

Shareefa M, Singh A K, Srivastav M and Dubey A K. 2009. Differentiation of nucellar and zygotic seedlings in citrus using ISSR markers. Indian Journal of Agricultural Science 79: 884–89.

Soni A, Dubey A K, Gupta A, Sharma R M, Awasthi O P, Bharadwaj C and Sharma N. 2019. Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange (Citrus aurantium) × Sacaton citrumelo (C. paradisi × Poncirus trifoliata) crosses through embryo rescue. Plant Breeding DOI: 10.1111/pbr.12690. DOI: https://doi.org/10.1111/pbr.12690

Spiegel-Roy P and Kochba J. 1980. Embryogenesis in Citrus tissue cultures. Advances in Biochemical Engineering 16: 27–48. DOI: https://doi.org/10.1007/3540098070_7

Tan M, Song J and Deng X. 2007. Production of two mandarin x trifoliate orange hybrid populations via embryo rescue with verification by SSR analysis. Euphytica 157: 155–60. DOI: https://doi.org/10.1007/s10681-007-9407-5

Turgutoglu E, Kurt S and Demir G. 2015. Effect of GA3 concentrations in basal medium on embryos germination of Cleopatra mandarin × Carrizo citrange and Cleopatra mandarin x Flying Dragon. Ekin Journal of Crop Breeding and Genetics 1: 17-19.

Yi H L, Deng X X and Fu C H. 2001. Application of embryo rescue techniques in fruit crops. Journal of Fruit Science 18(4): 224-28 (In Chinese).