Pollen storage of coconut dwarf accession Chowghat Orange Dwarf at low temperature

APARNA VELURU; KRISHNA PRAKASH; M NEEMA; K S MURALIKRISHNA; K SAMSUDEEN; K P CHANDRAN; M K RAJESH; ANITHA KARUN

doi:10.56093/ijas.v91i2.111649

Authors

APARNA VELURU ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India
KRISHNA PRAKASH ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India
M NEEMA ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India
K S MURALIKRISHNA ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India
K SAMSUDEEN ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India
K P CHANDRAN ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India
M K RAJESH ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India
ANITHA KARUN ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India

https://doi.org/10.56093/ijas.v91i2.111649

Keywords:

Coconut, Chowghat Orange Dwarf, Low-temperature storage, Pollen germination, Pollen storage

Abstract

Pollen collection and storage assume importance in hybridization programs in coconut. Development of an effective short-term storage method for coconut pollen ensures availability of pollen for coconut breeding throughout the year. In the present study, pollen collected from dwarf coconut cultivar Chowghat Orange Dwarf (COD), using standardized extraction procedures, was utilized for studying their storage behaviour under four different low-temperature regimes, i.e. 4ºC, -20ºC, -45ºC and -196ºC along with control at room temperature. Data on pollen germination percentage and pollen tube lengths were collected based on in vitro procedures at four different storage intervals (1st day, 14th day, 30th day and 60th day) to identify best short-term storage temperature. Germination percentage of pollen gradually reduced with duration of storage in all treatments but in case of pollen, which was stored at room temperature (control, i.e. 28±2ºC), viability decreased drastically and became nil within two weeks. Results revealed that higher germination percentage (38.82%) and pollen tube length (535.9 μm) were observed at -196ºC followed by -45ºC (31.77%, 400.73 μm) and -20ºC (30.82%, 282.89 μm) after a storage period of eight weeks. This study shows that desiccated pollen grains can be successfully stored at -45ºC for its utilization in breeding programmes besides long term storage at -196ºC.

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References

Akond A S M G M, Pounders C T, Blythe E K and Wang X. 2012. Longevity of crape myrtle pollen stored at different temperatures. Scientia Horticulturae 139: 53–7. DOI: https://doi.org/10.1016/j.scienta.2012.02.021

Du G, Xu J, Gao C, Lu J, Li Q, Du J, Lv M and Sun X. 2018. Effect of low storage temperature on pollen viability of fifteen herbaceous peonies. Biotechnology Reports 20: 17–21. DOI: https://doi.org/10.1016/j.btre.2019.e00309

Harrington J F. 1970. Seed and pollen storage for conservation of plant gene resources. Genetic Resources in Plants - Their Exploration and Conservation, pp 501–21. Frankel O H and Bennett E (Eds). Blackwell Scientific Publications, Oxford.

Karun A, Sajini K K, Meera N, Kumaran P M and Samsudheen K. 2006. Cryopreservation of coconut (Cocos nucifera L.). Journal of Plantation Crops 34: 568–71.

Karun A, Sajini K K, Niral V, Amaranath C H, Remya P, Rajesh M K, Samsudeen K, Jerard B A and Engelmann F. 2014. Coconut (Cocos nucifera L.) pollen cryopreservation. Cryo Letters 35(5): 407-17.

Kozaki I. 1975. Storage method of pollen. JIBP Synthesis V, Gene conservation-exploration, collection preservation and utilization, pp 89–94. Matsuo T (Ed). University Tokyo press, Tokyo.

Machado C A, Moura C R F, Lemos E E P, Ramos S R R, Riberio F E and Ledo A D S. 2014. Pollen grain viability of coconut accessions at low temperatures. Acta Scientiarum 36: 227–32. DOI: https://doi.org/10.4025/actasciagron.v36i2.17346

Mesnoua M, Roumani M and Salem A. 2018. The effect of pollen storage temperatures on pollen viability, fruit set and fruit quality of six date palm cultivars. Scientia Horticulturae 236: 279–83. DOI: https://doi.org/10.1016/j.scienta.2018.03.053

Tongkaemkaew U and Kaewbunjong S. 2017. Effect of culture medium and time storage on pollen germination and viability of Hevea clones. Khon Kaen Agriculture Journal 45(4): 761–66.

Wang M L, Hsu C M, Chang L C, Wang C S, Su T H, Huang Y J J, Jiang L and Jauh GY. 2004. Gene expression profiles of cold stored and fresh pollen to investigate pollen germination and growth. Plant and Cell Physiology 45: 1519–28. Fig 1 Pollen germination percentage and tube lengths at different low temperature treatments: Control, (T1) 4ºC, (T2) -20ºC, (T3) -45ºC and (T4) -196ºC after 60 days of storage. DOI: https://doi.org/10.1093/pcp/pch174