Optimization of in vitro micropropagation of ethnomedicinally important underexplored plant Maytenus emarginata

MAHENDRA SINGH; VIMALA YERRAMILLI; ISHWAR SINGH; DEEPTI SINGH

doi:10.56093/ijas.v91i4.112633

Authors

MAHENDRA SINGH Chaudhary Charan Singh University, Meerut 250 004, India
VIMALA YERRAMILLI Chaudhary Charan Singh University, Meerut 250 004, India
ISHWAR SINGH Chaudhary Charan Singh University, Meerut 250 004, India
DEEPTI SINGH Meerut College Meerut

https://doi.org/10.56093/ijas.v91i4.112633

Keywords:

BAP, Callus, GA3, Maytenus emarginata, NAA, Shoot emergence

Abstract

In vitro callus induction and shoot emergence protocols for Maytenus emarginata (Willd) Ding Hou. have been established using young leaves and stem explants of in vivo growing, one and half year-old plants. Plants were growing in the month of August 2016 at department of Botany, Ch. Charan Singh University, Meerut. However, explants were collected in the month of August, 2019. MS (Murashige and Skoog) medium and WPM (Woody Plant Medium), supplemented with different concentrations and combinations of plant growth regulators (PGRs) such as 0.5-2.0 mg/l Naphthalene acetic acid (NAA), 0.5-1.0 mg/l Benzyl Amino Purine (BAP), 2, 4-D (1.0-2.0 mg/l) and 1.0-1.5 mg/l Gibberellic acid (GA3) and temperature regimes were used for callus induction and shoot emergence. The presence of GA3 was found necessary for callus induction and shoot emergence from both explants (leaf and stem). The best response of callus formation from leaf explants was obtained at 24°C on WPM supplemented with 0.5 mg/l NAA+1.0mg/l BAP+1.5mg/l GA3 and MS medium supplemented with 2.0 mg/l NAA+ 0.5 mg/l BAP+ 1.0mg/l GA3. Stem explants exhibited best degree of callus induction only on MS medium supplemented with 0.5mg/l NAA+ 1.0 mg/l BAP+1.5 mg/l GA3. Light and temperature play very crucial role in shoot emergence because exposure of callus or nodal section of stem, to 12 hours dark period followed by 12 hours PAR (photosynthetically active radiation) at 27±2 °C induced shoot emergence on MS medium supplemented with 0.5mg/l NAA+1.0mg/l BAP+1.5 mg/l GA3 after 42 days. This is the first optimization report on tissue culture and medicinal use of callus of Maytenus emarginata.

Downloads

Download data is not yet available.

References

Dhar U and Upreti J.1999. In vitro regeneration of a mature leguminous liana (Bauhinia vahlii Wight and Arnott). Plant Cell Reports 18(7-8): 664–69.

Dos Santos V A, Dos Santos D P, Castro-Gamboa I, Zanoni MV and Furlan M.2010.Evaluation of antioxidant capacity and synergistic associations of Quinone-methidetriterpenes and phenolics substances from Maytenus ilicifolia (Celastraceae). Molecules 15(10): 6956–73.

Dos Santos V A, Leite K M, da Costa Siqueira M, Regasini L O, Martinez I, Nogueira C T, Galuppo M K, Stolf B S, Pereira A M, Cicarelli R M, Furlan M and Graminha MA.2013. Antiprotozoal activity of quinone-methidetriterpenes from Maytenusilicifolia (Celastraceae). Molecules 18(1): 1053–62.

Gullo F P, Sardi J C, Santos V A, Sangalli-Leite F, Pitangui N S, Rossi S A, de Paula E Silva A C, Soares L A, Silva F, Oliveira H C, Furlan M, Silva D H, Bolzani V S, Mendes-Giannini M J and Fusco-Almeida A M.2012. Antifungal activity of Maytenus and Pristimerin. Evidence-Based Complementary and Alternative Medicine.2012:1-6.

Gunatilak A A L.1996.Triterpenoidquinonemethides and related compounds (Celastroloids). (In) Progress in the Chemistry of Organic Natural Products, pp. 1–123. vol. 67 Springer-Verlag/ Wien: New York, NY, USA.

Hasnain S and Cheliak W.1986.Tissue culture in forestry: Economic and genetic potential. Forestry Chronicle 82: 219–225.

Khan Nadra, Ahmed Maqsood, Hafiz Ishfaq, Abbaai Nadeem, Ejaz S and Anjum M. 2015.Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes. Journal International des sciences de la vigne et du vin 49(1): DOI: https://doi.org/10.20870/oeno-one2015 49.1.95

Khichar M K, Manoj M and Agrawal R D. 2019. Maytenus emarginated: A folklore ethnomedicinal plant of Rajasthan. Journal of Drug Delivery & Therapeutics. 9(4-A):901-907 DIO: http://dx.doi.org/10.22270/jddt.v9i4-A.3832

Kim D H, Shiu E K, Kim Y H, Lee B W, Juu J G, Park J H X and Kim J K.2009. Suppresser of inflammatory responses of Celastrus a quinone-methidetriterpenoids isolated from Celastru sregelii. European Journal of Clinical Investigator 39(9): 819–29.

Lei Zhang, Ming-yueji, ,BinQiu, Qin-YuLi,Ke-Yong Zhang, Cheng Liu and Lian-Sheng Dang.2020. Phytochemical and biological activities of species from the genus Maytenus. Medicinal Chemistry Research DIO: https://doi.org/10.1007/s00044-020-02509-4

Mathur S and Goswami A.2014. Effect of precursor β-phenylalanine on production of flavonoids of Maytenus emarginata in vitro. International Journal of Science and Research 3(7) : 333-33.

Morita H, Hirasawa Y, Muto A, Yoshida T, Sekita S and Shirota O.2008.Antimitotic quinoidtriterpenes from Maytenus chuchuhuasca. Bioorganic and Medicinal Chemistry Letters 18(3):1050–52.

Murashige T and Skoog F.1962.Revised medium for rapid growth and bioassays for tobacco tissue cultures. Physiologia plantarum15(3):473–25.

Ngassapa O, Soejarto D D, Pezzuto J M and Farnsworth N R.1994.Quinone-methide triterpenes and salaspermic acid from Kokoonao chracea. Journal of Natural Products 57(1): 1–8.

Oramas-Royo S M, Chavez H, Martin-Rodriguez P, Fernandez- Perez L, Ravelo A G, and Estevez-Braun A. Cytotoxic triterpenoids from Maytenus retusa. 2010. Journal of Natural Products 73(12): 2029–34.

Paz T A, Santos dos Vania A F F M, Inacio M C, Pina E S, Pereira A M S and Furlan M. 2013 Production of the quinone-methide triterpene maytenin by in vitro adventitious roots of peritassa campestris (Cambess.) A.C.Sm. (Celastraceae) and rapid detection and identification by APCI-IT-MS/MS. Bio. Med. Research International 13 Article ID 485837 | https:// doi.org/10.1155/2013/485837

Rathore T S, Deora N S and Shehawat N S.1992. Cloning of Maytenus emarginata (Willd.) Ding Hou-a tree of the Indian desert, through tissue culture. Plant Cell Reports 11(9): 449–51.

Sangwan S, Rao D V and Sharma R A.2011. Maytenus emarginata (Willd.): A promising drug for cancer therapy. Asian Journal of Pharmaceutical and Clinical Research 4: 9–12.

Seo H R, Seo W D, Pyun B J, Lee B W, Jin Y B, Park K H, Seo E K, Lee Y J and Lee Y S.2011.Radiosensitization by celastrol is mediated by modification of antioxidant thiolmolecules. Chemico-Biological Interactions 193(1): 34–42.

Tao H, Jing X, Lina Y and Haitao W.2012. An efficient method for plant regeneration from calli of Swertia mussotii, an endangered medicinal herb. American Journal of Plant Sciences 3(7): 904-5.

Veloso C C, Soares G L, Perez A C, Rodrigues V G and Silva F C. 2017. Pharmacological potential of Maytenus species and isolated constituents, especially tingenone, for treatment of painful inflammatory diseases. Revista Brasileirade Farmacognosia 27(4): 533–40.