Antifungal metabolite profiling in Chaetomium globosum potential strain Cg2 effective against Bipolaris sorokiniana
311 / 229
Keywords:
Bipolaris sorokiniana, Biocontrol mechanism, MetabolitesAbstract
The fungus reported to be a potential antagonist of various soil and seed borne plant pathogens. C. globosum mycoparasitizes and produce antifungal metabolites which suppress the growth of the pathogenic fungi. Despite sufficient knowledge available on secondary metabolites of C. globosum and their wide biological activities, limited literatures are available on the polar active constituents and their potential use as antifungal agent. The study was carried out to evaluate chemical composition of secondary metabolites from C. globosum when it interacts with Bipolaris sorokiniana (2017-2020). The volatile compounds identified through GC-MS revealed that, C. globosum strain Cg2 produced a variety of antifungal secondary metabolites, i.e. octadecene trans-limonene oxide, dodecane, tetracosonal, heptacosanol and octadecanoic acid which may be involved in the antagonism. Similarly, UPLC-QToF-ESIMS analysis of chloroform soluble fraction of C. globosum (Cg2), B. sorokiniana (BS112) and their interaction (Cg2-BS112) were undertaken to identify non-volatile metabolites.These metabolites were identified as Chaetomugilin A, D, E, F, Globoxanthone A, Chaetoviridin A, B, E, Chaetoglobin B, Chaetoquadrin A, Chaetocochin B and F, Chaetoglobosin Q and N.The work indicates that the biocontrol agent C. globosum showed high antifungal metabolite production thereby antagonising B. sorokininana pathogen. The obtained data will greatly enrich current C. globosum metabolomic information and provide a good foundation for better understanding of biocontrol mechanism of C. globosum against plant pathogens and facilitating widespread application in the field of bio-control.Downloads
References
Aggarwal M, Shrivastava N and Padh H. 2008. Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Report 27: 617-631.
Aggarwal R, Gupta S, Singh V B and Sharma S. 2011. Microbial detoxification of pathotoxin produced by spot blotch pathogen Bipolaris sorokiniana infecting wheat. Journal of Plant Biochemistry and Biotechnology 20(1): 66-73.
Aggarwal R, Kharbikar L L, Sharma S, Gupta S and Yadav A. 2013. Phylogenetic relationships of Chaetomium isolates based on the internal transcribed spacer region of the rRNA gene cluster. African Journal of Biotechnology 12(9): 91420.
Aggarwal R, Singh V B, Shukla R, Gurjar M S, Gupta S and Sharma T R. 2010. URP-based DNA fingerprinting of Bipolaris sorokiniana isolates causing spot blotch of wheat. Journal of Phytopathology 158(4): 210-16.
Aggarwal R, Tewari A K, Srivastava K D and Singh D V. 2004. Role of antibiosis in the biological control of spot blotch (Cochliobolus sativus) of wheat by Chaetomium globosum. Mycopathologia 157(4): 369-77.
Bai H, Wu L, Yang T and Li G. 2015. Isolation and identification of secondary metabolites from fungus Chaetomium gracile and their antimicrobial activities. Chinese Journal of Applied and Environmental Biology 21: 274-78.
Biswas S K, Srivastava K D, Aggarwal R, Dureja P and Singh D V. 2000. Antagonism of Chaetomium globosum to Drechslera sorokiniana, the spot blotch pathogen of wheat. Indian Phytopathology 53: 436-40.
Darshan K, Aggarwal R, Bashyal B M, Singh J, Shanmugam V, Gurjar M S and Solanke A U. 2020. Transcriptome profiling provides insights into potential antagonistic mechanisms involved in Chaetomium globosum against Bipolaris sorokiniana. Frontiers in Microbiology 11: 578115.
Domsch K H, Gams W and Anderson T H. 1993. Compendium of Soil Fungi, 2nd ed. Academic Press, London, UK, p 672. Guo L, Wu J Z, Han T, Cao T, Rahman K and Qin LP. 2008. Chemical composition, antifungal and antitumor properties of ether extracts of Scapania verrucose Heeg. and its endophytic fungus Chaetomium fusiforme. Molecules 13(9): 2114-25.
Jiang C, Song J, Zhang J and Yang Q. 2017. Identification and characterization of the major antifungal substance against Fusarium sporotrichioides from Chaetomium globosum. World Journal of Microbiology and Biotechnology 33(6): 108.
Mandal S K, Srivastava D, Aggarwal R and Singh D V. 1999. Mycoparasitic action of some fungi on spot blotch pathogen Drechslera sorokiniana of wheat. Indian Phytopathology 52: 39-43.
Muroga, Y, Yamada T, NumataA and Tanaka R. 2010. 11- and 4′-Epimers of Chaetomugilin A, Novel cytostatic metabolites from marine fish-derived fungus Chaetomium globosum. Helvetica Chimica Acta 93(3): 542-49.
Pornsuriya C, Lin F C, Kanokmedhakul S, and Soytong K. 2008. New record of Chaetomium species isolated from soil under pineapple plantation in Thailand. Journal of Agricultural Technology 4(2): 91-103.
Qin J C, Zhang Y M, Gao J M, Bai M S, Yang S X and Laatsch H. 2009. Bioactive metabolites produced by Chaetomium globosum, an endophytic fungus isolated from Ginkgo biloba. Bioorganic and Medicinal Chemistry Letters 19(6): 1572-74.
Rajkumar E R, Aggarwal R and Singh B. 2005. Fungal antagonists for the biological control of Ascochyta blight of chickpea. Acta Phytopathologica et Entomologican Hungarica 40(1–2): 35–42.
Safe S and Taylor A. 1972. Sporidesmins. Part XIII. Ovine III–thrift in Nova Scotia. Part III. The characterisation of Chetomin a Toxic Metabolites of Chaetomium cochliodes and Chaetomium globosum. Journal of Chemical Society 1: 472–79.
Senthilkumar M, Gurumoorthi P and Janardhanan K. 2006. Some medicinal plants used by Irular, the tribal people of Marudhamalai hills, Coimbatore, Tamil Nadu.
Sharma S, Aggarwal R, Yadav A and Gupta S. 2014. Protein mapping of Chaetomium globosum, a potential biological control agent through proteomics approach. Journal of Plant Biochemistry and Biotechnology 23(3): 284-92.
Soytong K, Kanokmedhakul S, Kukongviriyapa V and Isobe M. 2001. Application of Chaetomium species (Ketomium) as a new broad-spectrum biological fungicide for plant disease control. Fungal Diversity 7: 1-15.
Von A J A, Guarro J and Figueras M J. 1986. The ascomycetes genus. (In) der Gebrüder Borntraeger Verlagsbuch hand lung Berlin. Stuttgart. Jim Cramer. 1-162.
Wang D, Zhang Y, Li X, Pan H, Chang M, Zheng T and Qin J. 2017. Potential allelopathicazaphilones produced by the endophytic Chaetomium globosum TY1 inhabited in Ginkgo biloba using the one strain− many compounds method. Natural Product Research 31(6): 724-28.
Yu FX, Chen Y, Yang Y H, Li GH and Zhao PJ. 2018. A new epi-polythiodioxopiperazine with antibacterial and cytotoxic activities from the endophytic fungus Chaetomium sp. M336. Natural product research 32(6): 689-94.
Zhang G, Zhang Y, Qin J, Qu X, Liu J and Li X. 2013. Antifungal metabolites produced by Chaetomium globosum No. 04, an endophytic fungus isolated from Ginkgo biloba. Indian Journal of Microbiology 53(2): 175-80.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2021 The Indian Journal of Agricultural Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.