Multivariate analysis in wheat germplasm captures variability for agro-morphological and physiological traits

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  • SATISH KUMAR ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, India
  • VIKAS GUPTA ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, India
  • SONU SINGH YADAV ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, India
  • MAMRUTHA H M ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, India
  • SANJAY KUMAR SINGH ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, India
  • RAVISH CHATRATH ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, India
  • GYANENDRA PRATAP SINGH ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, India


Agro-morphological traits, Principal Component Analysis, Variability, Wheat


The present study was carried out with an objective to investige genetic diversity in set of diverse wheat genotypes during 2018-19 at the experimental farm of Indian Institute of Wheat and Barley Research, Karnal. Principal Component Analysis was carried out on this set of 440 genotypes to study variability for different agro-morphological and physiological traits. The coefficient of variation ranged from 7.20-38.30 for the measured traits. Yield per plot was positively related with tillers per m, spike length, grains per spike and 1000 grains weight. The first three principal components explained 45.66% of variation. Agglomerative clustering grouped the genotypes into six groups and had a cophenetic correlation coefficient of 0.319. Almost all the components explained some variation for each trait and hence can be further used in hybridization for creation of variability in the breeding programs to develop improved cultivars. This remarkable variation in the set can be accounted to the fact that these lines were a collection of germplasm from different wheat growing countries and also their specificity for different traits.


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Amiri R, Bahraminejada S, Sasanib S, Jalali-Honarmanda S and Fakhric R. 2015. Bread wheat genetic variation for grain’s protein, iron and zinc concentrations as uptake by their genetic ability. European Journal of Agronomy 67: 20–26. DOI:

Ashraf A, Abd El- Mohsen, Samir R, Abo H and Moemen H T. 2012. Genotypic and phenotypic interrelationships among yield and yield components in Egyptian bread wheat genotypes. Journal of Plant Breeding and Crop Science 4(1): 9–16.

Badakhshan H, Moradi N, Mohammadzadeh H and Zakeri M R. 2013. Genetic variability analysis of grains Fe, Zn and Beta-carotene concentration of prevalent wheat varieties in Iran. International Journal of Agriculture and Crop Science 6(2): 57–62.

Bityutskii N, Yakkonen K and Loskutov I. 2017. Content of iron, zinc and manganese in grains of Triticum aestivum, Secale cereale, Hordeum vulgare and Avena sativa cultivars registered in Russia. Genetic Resources and Crop Evolution 64(8): 1955–61. DOI:

Brisson N, Gate P, Gouache D, Charmet G, François-Xavier O and Huard F. 2010. Why are wheat yields stagnating in Europe? A comprehensive data analysis for France. Field Crop Research 119: 201–12. DOI:

Cakmak I, Torun A, Millet E, Feldman M, Fahima T, Korol A B, Nevo E, Braun H J and Ozkan H. 2004. Triticum dicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Science and Plant Nutrition 50: 1047–54. DOI:

Dutamo D, Alamerew S, Eticha F and Assefa E. 2015. Genetic variability in bread wheat (Triticum aestivum L.) germplasm for yield and yield component traits. Journal of Biological and Agricultural Healthcare 5: 140–47.

Fu Y B and Somers D J. 2009. Genome-wide reduction of genetic diversity in wheat breeding. Crop Science 49: 161–68. DOI:

Govindaraj M, Vetriventhan M and Srinivasan M. 2015. Importance of genetic diversity assessment in crop plants and its recent advances: An overview of its analytical perspectives. Genetic Research International 3: 431–87. DOI:

Grassini P, Eskridge K M and Cassman K G. 2013. Distinguishing between yield advances and yield plateaus in historical crop production trends. Nature Communication 4: 2918. DOI:

Heal G B, Walker B, Levin S, Arrow K, Dasgupta P, Daily G, Paul E, Karl-Goran M N, Kautsky J L, Steve S and Starrett D. 2004. Genetic diversity and interdependent crop choices in agriculture. Research and Energy Ecosystem 26: 175–84. DOI:

IRRI. 2014. Statistical Tool for Agricultural Research (STAR) v. 2.0.1. Retrieved June 1, 2018, from

Kumar S, Singh S S, Mishra C N, Saroha M, Gupta V, Sharma P, Tiwari V and Sharma I. 2015. Assessment of tiller inhibition (tin) gene molecular marker for its application in marker-assisted breeding in wheat. National Academy Science Letters 38(6): 457–60. DOI:

Kumar S, Sandhu G, Yadav S S, Pandey V, Prakash O, Verma A, Bhardwaj S C, Chatrath R and Singh G P. 2019. Agro-morphological and molecular assessment of advanced wheat breeding lines for grain yield, quality and rust resistance. Journal of Cereal Research 11(2): 131–39. DOI:

Mishra C N, Tiwari V, Kumar A, Kumar S, Singh G and Singh G P. 2020. Physiological differences at different growth stages of wheat and their effect on yield and yield attributing traits. Journal of Cereal Research 12(3): 317–26. DOI:

Peleg Z, Cakmak I, Ozturk L, Yazici A, Jun Y, Budak H, Korol A B, Fahima T and Saranga Y. 2009. Quantitative trait loci conferring grain mineral nutrient concentrations in durum wheat × wild emmer wheat RIL population. Theoretical and Applied Genetics 119: 353–69. DOI:

Sajjad M, Khan S H and Khan A S. 2011. Exploitation of germplasm for grain yield improvement in spring wheat (Triticum aestivum). International Journal of Agricultural Biology 13: 695–700.

Samec D, Maretic M, Lugaric I, Mesic A, Salopek-Sondi B and Duralija B. 2016. Assessment of the differences in the physical, chemical and phytochemical properties of four strawberry cultivars using principal component analysis. Food Chemistry 194: 828–34. DOI:

Singh G P. 2020. Director’s Report of AICRP on Wheat and Barley 2019-20, ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India, p 76.

Smale MI, Ortiz-Monasterio M, Warburton B, Kovmand S, Reynolds M, Crossa J, Singh R and Trethowan R. 2002. Dimensions of diversity in modern spring bread wheat in developing countries from 1965. Crop Science 42: 1766–79. DOI:

Trethowan R, Chatrath R, Tiwari R, Kumar S, Saharan M S, Bains N S, Sohu V S, Srivastava P, Sharma A, De N, Prakash P, Singh G P, Sharma I, Eagles H, Diffey S, Bansal U and Bariana H S. 2018. An analysis of wheat yield and adaptation in India. Field Crop Research 219: 192-213. DOI:

Tripathi S N, Marker S, Pandey P, Jaiswal K K and Tiwari D K. 2011. Relationship between some morphological and physiological traits with grain yield in bread wheat (Triticum aestivum L.em.Thell.). Trends in Applied Science Research 6(9): 1037–45. DOI:

Van den Broeck H C, De Jong H C and Salentijn E M. 2010. Presence of celiac disease epitopes in modern and old hexaploid wheat varieties: wheat breeding may have contributed to increased prevalence of celiac disease. Theoretical and Applied Genetics 121(8): 1527–39. DOI:









How to Cite

KUMAR, S., GUPTA, V., YADAV, S. S., M, M. H., SINGH, S. K., CHATRATH, R., & SINGH, G. P. (2021). Multivariate analysis in wheat germplasm captures variability for agro-morphological and physiological traits. The Indian Journal of Agricultural Sciences, 91(9), 1322–1327.