Allelic and bio-chemical characterization of Indian wheat (Triticum aestivum) varieties and breeding lines for quality traits

DIVYA PHOUGAT; PREETI PREETI; VIKRAM SINGH; SHIKHA YASHVEER; I S PANWAR

doi:10.56093/ijas.v91i9.116095

Authors

DIVYA PHOUGAT Chaudhary Charan Singh Haryana Agricultural University, Hisar 125 001, India
PREETI PREETI Chaudhary Charan Singh Haryana Agricultural University, Hisar 125 001, India
VIKRAM SINGH Chaudhary Charan Singh Haryana Agricultural University, Hisar 125 001, India
SHIKHA YASHVEER Chaudhary Charan Singh Haryana Agricultural University, Hisar 125 001, India
I S PANWAR Chaudhary Charan Singh Haryana Agricultural University, Hisar 125 001, India

https://doi.org/10.56093/ijas.v91i9.116095

Keywords:

Allelic diversity, Biochemical traits, Characterization, Markers, wheat

Abstract

Thirteen released and two elite genotypes of wheat (Triticum aestivum L.) from North-West Plain Zone of India were subjected to biochemical and molecular characterization to study the genetic variability, their grouping and identification using a combination of biochemical and molecular markers during 2014-16 at Department of Genetics and Plant Breeding, COA, CCSHAU, Hisar. HD 2967, WH 1142 and WH 1080 were overall better performing for protein content, sedimentation value, hectolitre weight and grain appearance. A total of 99 and 117 polymorphic bands were detected using ISSR and SSR primers, respectively. The similarity indices for SSR ranged from 0.64 to 0.77 while ISSR showed the range of 0.63-0.92. Present study could be effectively utilized in DNA fingerprinting, identification of wheat varieties and elite lines for bio-chemical traits and determination of seed purity.

Downloads

Download data is not yet available.

References

Ayala M, Guzma´n C, Pena R J and Alvarez J B. 2016. Diversity of phenotypic (plant and grain morphological) and genotypic (glutenin alleles in Glu-1 and Glu-3 loci) traits of wheat landraces (Triticum aestivum) from Andalusia (Southern Spain). Genetic Resources and Crop Evolution 63: 465–75. DOI: https://doi.org/10.1007/s10722-015-0264-0

Dale Z, Yuhao Y, Yarui S and Suoping L. 2016. Analysis of dough rheological property and gluten quality characteristics in wild emmer wheat (Triticum dicoccoides (Korn. ex Asch. et Graebn.) Schweinf.). Genetic Resources and Crop Evolution 63: 675–683. DOI: https://doi.org/10.1007/s10722-015-0275-x

Doyle J J and Doyle J L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15. DOI: https://doi.org/10.2307/2419362

Drikvand R M, Najafian G, Elham and Salahvarzi A. 2015. Investigation of genetic diversity of some durum wheat genotypes using SSR markers. Journal of Biodiversity and Environmental Sciences 6(3): 24–32.

ICAR-IIWBR 2017. Progress Report of AICRP on Wheat and Barley 2016-17., Wheat Quality. R K Gupta, D Mohan, Sewa Ram, Sneh Narwal, Vanita Pandey and Gyanendra Pratap Singh (Eds). ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India, p 176.

Phougat D and Sethi S K. 2020. SSR markers based genetic diversity analysis of biofortified bread wheat (Triticum aestivum L. em. Thell) crosses. Indian Journal of Agricultural Sciences 90(4): 1–8.

Preeti, Singh V, Yashveer S, Panwar I S and Phougat D. 2016 Evaluation of phenotypic and molecular diversity in wheat cultivars and advanced breeding lines of wheat in India. Research on Crops 17(3): 605–12 DOI: https://doi.org/10.5958/2348-7542.2016.00101.7

Rohlf F J. 1990: NTSYS-PC numerical taxonomy and multivariate analysis system. Exeter Software.

Sammour R H.1991. Using electrophoretic techniques invarietal identification, biosystematic analysis, phylogenetic relations and genetic resources management. Journal of Islamic Academy of Sciences 43: 221–26.

Sheoran S, Sharma P, Singh V, Pawar S, Sharma D, Jain N, Kumar R, Thakur V, Pandey G C, Malik R, Tiwari R, Tiwari V, Gupta R K and Sharma I. 2015: Assessment of genetic diversity in elite wheat genotypes using simple sequence repeat and quality protein markers. Journal of Wheat Research 7(1): 8–26.

Shewry P R, Halford N G , Tatham A S , Popineau Y , Lafiandra D and Belton P S. 2003. The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Advances In Food and Nutrition Research 45: 221–302. DOI: https://doi.org/10.1016/S1043-4526(03)45006-7

Singh G, Kumar P, Kumar R and Gangwar L K 2018. Genetic diversity analysis for various morphological and quality traits in bread wheat (Triticum aestivum L.). Journal of Applied and Natural Science 10(1): 24–29 DOI: https://doi.org/10.31018/jans.v10i1.1572

.

Teklu Y, Hammer K and Roder M S. 2007. Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon Schrank): Analysis of genetic diversity and differentiation. Genetic Resources and Crop Evolution 54: 543–54. DOI: https://doi.org/10.1007/s10722-006-0011-7