Validation of genome-data based simple sequence repeats for ascertaining hybridity in mango (Mangifera indica) hybrids


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Authors

  • MEGHA R Division of Fruits and Horticultural techology, ICAR-IARI, New Delhi
  • SANJAY K SINGH Prof. & Head, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 https://orcid.org/0000-0002-2356-5157
  • MANISH SRIVASTAV Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012
  • JAI PRAKASH Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012
  • CHAVLESH KUMAR Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012
  • SRIDHAR R Department of Fruit Science, College of Horticulture, Hiriyur, Chitradurga, Karnataka
  • N K SINGH ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • NEHA SHARMA Department of Biotechnology, IILM College of Engineering & Technology, Greater Noida, Uttar Pradesh

https://doi.org/10.56093/ijas.v92i8.120475

Keywords:

Mango, hybrids, hypervariable SSRs, genetic diversity

Abstract

 The present study was carried to identify polymorphic SSRs derived from the whole draft genome data of mango (Mangifera indica L.) for their polymorphism between Amrapali and Sensation genotypes (parents) and their validation on the derived hybrid progenies. Fifty new hypervariable mango SSRs (HMSSRs) were screened against the parental genotypes. Out of which, 11 HMSSRs were polymorphic, 18 were monomorphic and 21 did not amplify at all. These polymorphic HMSSRs were then used for ascertaining the hybridity of the hybrid progenies. The genetic diversity indices of each SSR locus among mango hybrids were calculated using Power Marker ver. 3.25. The genetic diversity indices such as the number of alleles ranged from 2 (HMSSR35) to 5 (HMSSR57), gene diversity from 0.36 (HMSSR54) to 0.71 (HMSSR57 & HMSSR58), and PIC values from 0.35 (HMSSR69) to 0.66 (HMSSR57). The dendrogram generated using Neighbor joining tree divided the 22 genotypes into three distinct clades, viz. Clade ‘A’, Clade ‘B’ and Clade ‘C’. The hybrids, viz. H-3-1, H-3-2, and H-7-4 were identified as true hybrids, since they were genetically more close to female parent (Amrapali), while hybrids H-3-3, H-3-11, H-11-1, H-12-5, H-1-6, H-1-13, H-12-8, H-12-10, H-3-12, H-1-1 and H-4-10 were more close to the male donor parent (Sensation) and also had red peel colour. In the present study, the 20 hybrids were confirmed to be the true hybrids since the bands unique to the two parents were present in them.

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Author Biographies

  • MEGHA R, Division of Fruits and Horticultural techology, ICAR-IARI, New Delhi

    Research Scholar, Fruit science

  • MANISH SRIVASTAV, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012

    Prof & Principal Scientist, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012

  • JAI PRAKASH, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012

    Principal Scientist

  • CHAVLESH KUMAR, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012

    Scientist

  • SRIDHAR R, Department of Fruit Science, College of Horticulture, Hiriyur, Chitradurga, Karnataka

    Research Scholar, Fruit Science

  • NEHA SHARMA, Department of Biotechnology, IILM College of Engineering & Technology, Greater Noida, Uttar Pradesh

    Senior Research Fellow

References

Begum H, Reddy M T, Malathi S, Reddy P, Narshimulu G, Nagaraju J and Siddiq E A. 2014. Morphological and microsatellite analysis of intravarietal variability in ‘Cherukurasam’ cultivar of mango (Mangifera indica L.). Jordan Journal of Agricultural Sciences 10(3): 452–71.

Dillon N L, Innes D J, Bally I S E, Wright C L, Devitt L C and Dietzgen R G. 2014. Expressed sequence tag-simple sequence repeat (EST-SSR) marker resources for diversity analysis of mango (Mangifera indica L.). Diversity 6(1): 72–87.

Doyle J J and Doyle J L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19: 11–15.

Gálvez-López D, Hernández-Delgado S, González-Paz M, Becerra- Leor E N, Salvador-Figueroa M and Mayek-Pérez N. 2009. Genetic analysis of mango landraces from Mexico based on molecular markers. Plant Genetic Resources 7(3): 244–51.

Kalia R K, Rai M K, Kalia S, Singh R and Dhawan A K. 2011. Microsatellite markers: an overview of the recent progress in plants. Euphytica 177(3): 309–34.

Kumar K, Srivastava M, Singh S K and Kumar V. 2016. Ascertaining hybridity of progenies in mango (Mangifera indica L.) using microsatellite (SSR) markers. Journal of Agriculture and Ecology 2: 1–10.

Lakhotia P, Dinesh M R and Ravishankar K V. 2016. Validation of hybridity in mango (Mangifera indica L.) through markers. BIOINFOLET-A Quarterly Journal of Life Sciences 13(1b): 145–48.

Litz R E. 2003. The mango: botany, production, and uses. Tropical Research and Education Centre, University of Florida, USA. CAB International, Wallingford, United Kingdom, pp 587.

Olano C T, Schnell R J, Quintanilia W E and Campbell R J. 2005. Pedigree analysis of Florida mango cultivars. (In) Proceedings of the Florida State Horticultural Society, pp. 192–97.

Rao M N, Soneji J R, Chen C, Huang S and Gmitter F G. 2008. Characterization of zygotic and nucellar seedlings from sour orange-like citrus rootstock candidates using RAPD and EST-SSR markers. Tree Genetics and Genomes 4(1): 113–24.

Ravishankar K V, Bommisetty P, Bajpai A, Srivastava N, Mani B H, Vasugi C and Dinesh M R. 2015. Genetic diversity and population structure analysis of mango (Mangifera indica) cultivars assessed by microsatellite markers. Trees 29(3): 775–83.

Ravishankar K V, Padmakar B, Lavanya B, Mani B H and Dinesh M R. 2017. Development and characterization of microsatellite loci from mango (Mangifera indica L.). Indian Journal of Biotechnology 16(2): 250–53.

Razak S A B, Ariffin M A T, Mohamad S M S, Azman N H E N, Hassan M A and Sarip J. 2020. Microsatellite markers for the molecular characterization of potentially commercial mango (Mangifera indica) progenies. Malaysian Applied Biology 49(3): 81–85.

Samal K C, Jena R C, Swain S S, Das B K and Chand P K. 2012. Evaluation of genetic diversity among commercial cultivars, hybrids and local mango (Mangifera indica L.) genotypes of India using cumulative RAPD and ISSR markers. Euphytica 185(2): 195–13.

Shamili M, Fatahi R and Hormaza J I. 2012. Characterization and evaluation of genetic diversity of Iranian mango (Mangifera indica L.) genotypes using microsatellites. Scientia Horticulturae 148: 230–34.

Singh A, Singh A K and Singh S K. 2012. SSR markers reveal genetic diversity in closely related mango hybrids. Indian Journal of Horticulture 69(3): 299–05.

Singh A. 2015. ‘Bio-hardening and hybridity analysis of in vitro embryo cultured mango (Mangifera indica L.) plantlets’. PhD Thesis, Division of Fruits and Horticultural Technology ICAR-Indian Agricultural Research Institute New Delhi.

Srivastava A P, Chandra R and Ranade S A. 2004. Applicability of PCR based molecular markers for parentage analysis of mango (Mangifera indica L.) hybrids. Indian Journal of Genetics and Plant Breeding 64(4): 275–80.

Subashini V, Shanmugapriya A and Yasodha R. 2014. Hybrid purity assessment in Eucalyptus F1 hybrids using microsatellite markers. Biotechnology 4(4): 367–73.

Tang J, Zhou S, Cheng M, Lin Q and Zhou Z. 2006. Identification of the F1 hybrids of Malus xiaojinensis using RAPD and SSR molecular markers. Chinese Agricultural Science Bulletin 2: 1–10.

Yamanaka S, Hosaka F, Matsumura M, Onoue-Makishi Y, Nashima K, Urasaki N and Yamamoto T. 2019. Genetic diversity and relatedness of mango cultivars assessed by SSR markers. Breeding Science 69(2): 332–44.

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Submitted

2022-01-24

Published

2022-04-13

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How to Cite

R, M., SINGH, S. K., SRIVASTAV, M., PRAKASH, J., KUMAR, C., R, S., SINGH, N. K., & SHARMA, N. (2022). Validation of genome-data based simple sequence repeats for ascertaining hybridity in mango (Mangifera indica) hybrids. The Indian Journal of Agricultural Sciences, 92(8), 1001-1004. https://doi.org/10.56093/ijas.v92i8.120475
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