Inheritance and mapping of QTLs for Ascochyta blight resistance in chickpea (Cicer arietinum)


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Authors

  • DIVYA LEKHI ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • AJINDER KAUR ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • UPASANA RANI ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • SANGEETA SOI ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • INDERJIT SINGH ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • SHAYLA BINDRA ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • ADITYA PRATAP ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • SARVJEET SINGH ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • C BHARADWAJ ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v92i3.122677

Keywords:

Ascochyta blight, Chickpea, Linkage, Molecular mapping, QTLs

Abstract

There are very limited studies on the QTLs governing Ascochyta blight resistance which is a major problem in chickpea (Cicer arietinum L.) grown in North West Plain Zone of India in the cooler climates and world over. An interspecific mapping population consisting of 157 F2:3 lines developed by crossing a resistant parent ILWC129 (C. reticulatum) with a susceptible variety GL769 (C. arietinum L.), was evaluated (2019–20) and genotyped (2021) for genetic studies and mapping of resistance to Ascochyta rabiei (syn. Phoma rabiei), isolate 8(3968). The inheritance pattern gave 1R:2H:1S goodness of fit ratio with χ2 value of 0.4 indicating that resistance against isolate 8 of the pathogen governed by a single gene. Out of 831 SSR markers screened, 86 were found polymorphic with 10.3% polymorphism from which only 41 markers segregated in 1:2:1 ratio and could be mapped. Eight linkage groups with a cumulative distance of 1042.31 cM were formed with an average distance of 25.42 cM. This is probably the first report of inheritance studies and tagging of A. rabei race 8 (arr8) resistance gene using molecular markers in an interspecific cross with C. reticulatum.

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References

Bharadwaj C, Chauhan S K, Yadav S, Satyavathi C T, Singh R, Kumar J, Srivastava R and Rajguru G. 2011a. Molecular marker-based linkage map of chickpea (cicer arietinum) developed from desi × kabuli cross. Indian Journal of Agricultural Sciences 81(2): 116–18.

Bharadwaj C, Srivastava R, Chauhan S K, Satyavathi C T, Kumar J, Faruqui A, Yadav S, Rizvi A H and Kumar T. 2011b. Molecular diversity and phylogeny in geographical collection of chickpea (Cicer sp.) accessions. Journal of Geneitcs 90: e94–e100. DOI: https://doi.org/10.1007/s12041-011-0114-6

Choudhary P, Suruchi M Khanna, Pradeep K Jain, Chellapilla Bharadwaj, Jitendra Kumar, Pramesh C. Lakhera and Ramamurthy Srinivasan. 2013. Molecular characterization of primary gene pool of chickpea based on ISSR markers. Biochemical genetics 51(3-4): 306–22 DOI: https://doi.org/10.1007/s10528-012-9564-7

Garg T, Mallikarjuna B P, Thudi M, Srinivasan S, Singh S, Sandhu J S, Kaur L, Singh I, Sirari A, Basandrai A, Basandrai D, Varshney R K and Gaur P M. 2018. Identification of QTLs for resistance to Fusarium wilt and Ascochyta blight in a recombinant inbred population of chickpea (Cicer arietinum L.). Euphytica 214(3): 1–11. DOI: https://doi.org/10.1007/s10681-018-2125-3

Gurha S N, Singh G and Sharma Y R. 2003. Diseases of Chickpea and their Management, pp 195–227. Ali M, Kumar S and Singh N B (Eds). Chickpea Research in India. Indian Institute of Pulses Research, Kanpur

Kosambi D D. 1944. The estimation of map distances from recombination values. Annals of Eugenics 12: 505–25. DOI: https://doi.org/10.1111/j.1469-1809.1943.tb02321.x

Lichtenzveig J, Scheuring C, Dodge J, Abbo S and Zhang H B. 2005. Construction of BAC and BIRAC libraries and their applications for generation of SSR markers for genome analysis of chickpea, Cicer arietinum L. Theoretical and Applied Genetics 110: 492–510. DOI: https://doi.org/10.1007/s00122-004-1857-8

Nayak S N, Zhu H, Varghese N, Datta S, Choi H K, Horres R, Jüngling R, Singh J, Kishor P B, Sivaramakrishnan S, Hoisington D A, Kahl G, Winter P, Cook D R and Varshney R K. 2010. Integration of novel SSR and gene-based SNP marker loci in the chickpea genetic map and establishment of new anchor points with Medicago truncatula genome. Theoretical and Applied Genetics 120(7): 1415–41. DOI: https://doi.org/10.1007/s00122-010-1265-1

Sharma M and Ghosh R. 2016. An update on genetic resistance of chickpea to ascochyta blight. Agronomy 6(1): 18. DOI: https://doi.org/10.3390/agronomy6010018

Singh G, Sharma Y R and Bains T S. 1998. Status of Botrytis grey mold of chickpea research in Punjab, India. Recent Advances in Research and Management of Botrytis Grey Mold of Chickpea, pp. 7–14. S Pande, M A Bakr and C Johansen (Eds) (ICRISAT: Patancheru, AP, India).

Singh K B and Reddy M V. 1990. Patterns of resistance and susceptibility to races of Ascochyta rabiei among germplasms and breeding lines of chickpea. Plant Disease 74: 127–29. DOI: https://doi.org/10.1094/PD-74-0127

Subodh Verma, Gupta S, Bandhiwal N, Kumar T, Bharadwaj C and Bhatia S. 2015. High-density linkage map construction and mapping of seed trait QTLs in chickpea (Cicer arietinum L.) using Genotyping-by-Sequencing (GBS). Scientific reports 5(1): 1–14 DOI: https://doi.org/10.1038/srep17512

Thudi M, Bohra A, Nayak S N, Varghese N, Shah T M, Varma R P, Nepolean T, Gudipati S, Gaur P M, Kulwal P L, Upadhyaya H D, KaviKishor P B, Winter P, Kahl G, Town C D, Kilian A, Cook D R and Varshney R K. 2011. Novel SSR markers from BAC-End sequences, DArT arrays and a comprehensive genetic map with 1,291 marker loci for chickpea (Cicer arietinum L.). PLoS One 6(11), p.e27275 DOI: https://doi.org/10.1371/journal.pone.0027275

Voorrips R E .2002. MapChart: software for the graphical presentation of linkage maps and QTLs. Journal of Heredity 93: 77–78. DOI: https://doi.org/10.1093/jhered/93.1.77

Winter P, Pfaff T, Udupa S M, Huttel B, Sharma P C, Sahim S, Arreguin-Espinoza R, Weigand F, Muehlbauer F J and Kahl G. 1999. Characterization and mapping of sequence tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Molecular and General Genetics 262: 90–101. DOI: https://doi.org/10.1007/s004380051063

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Submitted

2022-03-28

Published

2022-03-29

Issue

Vol. 92 No. 3 (2022)

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Articles

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How to Cite

LEKHI, D., KAUR, A., RANI, U., SOI, S., SINGH, I., BINDRA, S., PRATAP, A., SINGH, S., & BHARADWAJ, C. (2022). Inheritance and mapping of QTLs for Ascochyta blight resistance in chickpea (Cicer arietinum). The Indian Journal of Agricultural Sciences, 92(3), 316-319. https://doi.org/10.56093/ijas.v92i3.122677
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