Molecular profiling of multi-coloured flesh potato (Solanum tuberosum) hybrids and interspecific somatic hybrids using SSR markers

JAGESH  KUMAR TIWARI; SATISH  K LUHRA; DALAMU; RASNA  ZINTA; TANUJA  BUCKSETH; RAJESH  K SINGH; MANOJ  KUMAR

doi:10.56093/ijas.v92i4.123964

Authors

JAGESH KUMAR TIWARI ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India
SATISH K LUHRA ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India
DALAMU ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India
RASNA ZINTA ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India
TANUJA BUCKSETH ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India
RAJESH K SINGH ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India
MANOJ KUMAR ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India

https://doi.org/10.56093/ijas.v92i4.123964

Keywords:

Genetic fidelity, Multi-coloured potato, Somatic hybrids, SSR markers

Abstract

The objective of this study was to develop SSR profiles of potato (Solanum tuberosum L.) hybrids for genetic fidelity purpose. The multi-coloured flesh potato hybrids and interspecific somatic hybrids-derived progenies were for the study conducted at ICAR-CPRI, Shimla during 2019–20. A total of 165 potato genotypes were analysed using two well-known potato SSR markers (STU6SNRN and STIIKA). High polymorphism was observed in STIIKA (PIC: 0.93) than STU6SNRN (PIC: 0.82), and higher number of alleles were observed in STIIKA (23) than STU6SNRN (7). In STU6SNRN, alleles size 174, 179, 182, 190 and 200 bp were predominant whereas in STIIKA, alleles size 191, 195, 198, 201, 221, 223, 231, 242, 245 and 256 were observed frequently in more than 50% of the genotypes.
Diversity analysis showed a clear distinction among the genotypes based on the Jaccard dissimilarity coefficient by the Neighbour-joining tree method using the DARwin software. SSR fingerprints would be valuable resources to strengthen genetic fidelity of these hybrids and identification of true-to-type clones.

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