Molecular characterization of monopartite bhendi (Abelmoschus moschatus) yellow vein mosaic virus and screening of wild okra


Abstract views: 98 / PDF downloads: 138

Authors

  • POOJA KUMARI ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • S P SINGH National Research Centre for Integrated Pest Management, New Delhi
  • K K GANGOPADHYAY ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • V C CHALAM ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, India
  • Y B BASAVARAJ ICAR-Indian Agricultural Research Institute, New Delhi
  • V VENKATARAVANAPPA Central Horticultural Experiment Station Chettalli, ICAR-Indian Institute of Horticultural Research, Chettalli, Karnataka
  • ASHWINI KUMAR ICAR-Indian Agricultural Research Institute, New Delhi

https://doi.org/10.56093/ijas.v92i11.125370

Keywords:

Alphasatellite, Begomovirus, Betasatellite, Bhendi yellow vein mosaic disease, Wild okra

Abstract

Bhendi yellow vein mosaic disease (BYVMD) is the most devastating viral disease of okra (Abelmoschus moschatus ssp. moschatus) which affects yield and quality of the produce in India. The causative agent of BYVMD is begomovirus i.e. bhendi yellow vein mosaic virus (BYVMV) having DNA-A molecule along with betasatellite. Present study was carried out during 2019–21 at research farm of ICAR-National Bureau of Plant Genetic Resources, New Delhi to find out the diversity of begomovirus and its satellite nucleotide sequences derived from wild okra infected samples exhibiting conspicuous symptom of BYVMD using PCR-based detection technique on two wild okra symptomatic samples, viz. EC361170 and EC361148. Full length amplification of BYVMV satellite molecule and partial amplification of DNA-A was carried out using PCR and cloning of both randomly selected samples showed the presence of monopartite BYVMV. In both samples, presence of DNA-A molecule, betasatellite and alphasatellite were noticed. Two year (kharif 2019 and 2021) field screening of 10 accessions of wild okra (Abelmoschus moschatus ssp. moschatus) was carried out at ICAR-NBPGR, New Delhi. Out of 10 accessions, viz. EC360586, EC360794, EC360830, EC360900, EC359730, EC359836, EC359870, EC360351, EC361111 and EC361171 screened, 4 accessions, viz. EC360794, EC360586, EC360830 and EC361171 showed resistant (R) reaction during both the seasons against BYVMD. This is the first study which showed the presence of alpha-satellite molecule of BYVMV from New Delhi region in wild okra along with its resistance source.

Downloads

Download data is not yet available.

References

Berrie L C, Rybicki E P and Rey M E C. 2001. Complete nucleotide sequence and host range of South African cassava mosaic virus: further evidence for recombination amongst begomoviruses. Journal of General Virology 82(1): 53–58.

Briddon R W, Mansoor S, Bedford I D, Pinner M S, Saunders K, Stanley J, Zafar Y, Malik K A and Markham P G. 2001. Identification of DNA components required for induction of cotton leaf curl disease. Virology 285: 234–43.

Briddon R W, Bull S E, Mansoor S, Amin I and Markham P G. 2002. Universal primers for the PCR-mediated amplification of DNA β. Molecular biotechnology 20(3): 315–18.

Briddon R W and Stanley J. 2006. Subviral agents associated with plant single-stranded DNA viruses. Virology 344(1): 198–210.

Bull S E, Briddon R W, Sserubombwe W S, Ngugi K, Markham P G and Stanley J. 2006. Genetic diversity and phylogeography of cassava mosaic viruses in Kenya. Journal of General Virology 87(10): 3053–65.

Capoor S P and Varma P M. 1950. Yellow vein mosaic of Abelmoschus esculentus (L.) Indian Journal of Agricultural Sciences 20: 217–30.

Doyle J J and Doyle J L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.

Fauquet C M and Stanley J. 2005. Revising the way we conceive and name viruses below the species level: a review of geminivirus taxonomy calls for new standardized isolate descriptors. Archives of virology 150(10): 2151–79.

Jose J and Usha R. 2003. Bhendi yellow vein mosaic disease in India is caused by association of a DNAβ satellite with a begomovirus. Virology 305: 310–17.

Kulkarni C S. 1924. Mosaic and other related diseases of crops in the Bombay Presidency. Poona Agric Coll Mag 6: 12.

Kumar S, Stecher G, Li M, Knyaz, C and Tamura K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution 35(6): 1547.

Mayee C D and Datar V V. 1986. Phytopathometery, pp. 84. Marathwada Agricultural University, Parbhani, University Press.

Muhire B M, Varsani A and Martin D P. 2014. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PloS one 9(9): e108277.

Polston J E and Anderson P K. 1997. The emergence of whitefly transmitted geminiviruses in tomato in Western Hemisphere. Plant Disease 81: 1358–69.

Pun K B and Doraiswamy S. 1999. Screening of plant species for the presence of antiviral principles against Okra yellow vein mosaic virus. Indian Phytopathology 52: 221–23.

Sastry K S M and Singh S J. 1974. Effect of yellow vein mosaic virus infection on growth and yield of okra crop. Indian Phytopathology 27: 294–97.

Saunders K, Bedford I D, Briddon R W, Markham P G, Wong S M and Stanley J. 2000. A unique virus complex causes Ageratum yellow vein disease. Proceedings of the National Academy of Sciences 97(12): 6890–95.

Schnippenkoetter W H, Martin D P, Willment J A and Rybicki E P. 2001. Forced recombination between distinct strains of Maize streak virus. Journal of General Virology 82(12): 3081–90.

Singh S J. 1980. Studies on the epidemiology of yellow vein mosaic virus of okra. Indian Journal of Mycology and Plant Pathology 10: 35–42.

Usha R. 1980. Characterization, Diagnosis and Management of Plant Viruses, pp. 387–92. G P Rao, P L Kumar, R L Holguin- Peria (Eds). Studium Press, Houston.

Venkataravanappa V, Lakshminarayana Reddy C N, Swaranalatha P, Jalali S, Briddon R W and Reddy M K. 2011. Diversity and phylogeography of begomovirus-associated beta satellites of okra in India. Virology journal 8(1): 1–13.

Venkataravanappa V, Lakshminarayana Reddy C N, Salil Jalali and Krishna Reddy M. 2012. Molecular characterization of distinct bipartite begomovirus infecting bhendi (Abelmoschus esculentus L.) in India. Virus Genes 44(3): 522–35.

Zhou Y C, Nonssourou M, Kon M R, Rajas H, Jiang, Chen LF, Gamby K, Foster R and Gilbertson R L. 2008. Evidence of local evolution of tomato-infecting begomovirus species in West Africa: characterization of tomato leaf curl Mali virus and tomato yellow leaf crumple virus from Mali. Archives of Virology 153(4): 693–706.

Downloads

Submitted

2022-07-02

Published

2022-11-11

Issue

Section

Articles

How to Cite

KUMARI, P., SINGH, S. P., GANGOPADHYAY, K. K., CHALAM, V. C., BASAVARAJ, Y. B., VENKATARAVANAPPA, V., & KUMAR, A. (2022). Molecular characterization of monopartite bhendi (Abelmoschus moschatus) yellow vein mosaic virus and screening of wild okra. The Indian Journal of Agricultural Sciences, 92(11), 1369–1374. https://doi.org/10.56093/ijas.v92i11.125370
Citation