First report of Paraphaeosphaeria angularis as endophyte in sugarcane (Saccharum officinarum) from India

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  • K MALARVIZHI Kanchi Mamunivar Govt. Institute for Postgraduate Studies and Research, Puducherry
  • T S MURALI Manipal Academy of Higher Education, Manipal, Karnataka
  • V KUMARESAN Kanchi Mamunivar Govt. Institute for Postgraduate Studies and Research, Puducherry


Endophyte, Paraphaeosphaeria, Saccharum, Sterile form


Sugarcane (Saccharum officinarum L.) is considered to be an important crop that is utilized for production of molasses, sugar and ethanol. Thus, identifying microbes associated with this crop plant will give more insight into plant-microbe interaction. Further, there are limited studies on sugarcane fungal endophytes. Therefore, sugarcane leaves obtained from approximately 3 month old sugarcane plants growing in Puducherry were screened for the presence of fungal endophytes, during March 2022. The study conducted to identify fungal endophytes of sugarcane resulted in isolation of a sterile form which was identified as Paraphaeosphaeria angularis, an ascomycetous fungal species, based on sequencing of the internal transcribed spacer and the 5.8S rDNA region. Maximum Likelihood method was used to infer the evolutionary history. It is the first report of this fungus, as endophyte, from sugarcane host from India. The genus Paraphaeosphaeria is known to include plant pathogens, biocontrol agents and endophytic fungi. This study highlights the importance of studying and assigning taxa to the sterile forms especially from crop plants, since a species belonging to Paraphaeosphaeria, viz. P. michotii is reported as a pathogen from sugarcane from other countries. Further studies will reveal the potential of P. angularis as fungal species known to occur just as an endophyte or a capable pathogen.


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Ariyawansa H A, Tsai I, Thambugala K M, Chuang W-Y, Lin S-R, Hozzein W N and Cheewangkoon R. 2020. Species diversity of Pleosporalean taxa associated with Camellia sinensis (L.) Kuntze in Taiwan. Scientific Reports 10: 12762.

Baroncelli R, Da D, Lio G and Sarrocco S. 2020. Genome resources for the endophytic fungus Paraphaeosphaeria sporulosa. Molecular Plant-Microbe Interactions 33: 1098–99.

de Amorim M R, Paz T A, Pinto L, Hilário F, Zanini C L, Aguiar A, Silva D, Furlan M, Guido R, Bauab T M, Netto A and Dos Santos L C. 2022. New isoquinoline alkaloids from Paraphaeosphaeria sporulosa F03, a fungal endophyte isolated from Paepalanthus planifolius. Planta medica 88(12): 994–003.

de Souza R S C, Okura V K, Armanhi J S L, Jorrin B, Lozano N, da Silva M J, Gonzale-Guerrero M, de Araujo L M, Verza N C, Bagheri H C, Imperial J and Arruda P. 2016. Unlocking the bacterial and fungal communities assemblages of sugarcane microbiome. Scientific Reports 6: 28774.

Ewing C J, Slot J, Benítez Ponce M-S, Rosa C, Malacrinò A, Bennett A and Bonello A. 2021. The foliar microbiome suggests fungal and bacterial agents may be involved in the beech leaf disease pathosystem. Phytobiomes Journal 5: 335–49.

Fearer C J, Conrad A O, Marra R E, Georskey C, Villari C, Slot J and Bonello P. 2022. A combined approach for early in-field detection of beech leaf disease using near-infrared spectroscopy and machine learning. Frontiers in Forest and Global Change 5: 934545.

Fors R O, Patreze C M, Louro Berbara R L, Carbone Carneiro M A and Saggin-Junior O J. 2020. Dark septate endophytic fungi associated with sugarcane plants cultivated in São Paulo, Brazil. Diversity 12: 351.

Gardes M and Bruns T D. 1993. ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118.

Guo L D, Hyde K D and Liew E C Y. 2000. Taxonomic placement of fungal endophytes from Livistona chinensis. (In) XVI International Botanical Congress, St. Louis, USA, August 1–7, Abstract No. 3117.

Khunnamwong P, Surussawadee J, Jindamorakot S and Limtong S. 2014. Wickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf. International Journal of Systematic and Evolutionary Microbiology 64: 3849–55.

Lufeng L I U, Haichun C U N, Pengfei H E, Yining D I, Yixin W U, Lilian H E, Fusheng L I and Yueqiu H E. 2019. Isolation, identification and multiple function analyses of sugarcane endophytes. Chinese Journal of Tropical Crops 40: 1144–52.

Marques L L M, Ferreira E D F, de Paula M N, Klein T and de Mello J C P. 2019. Paullinia cupana: a multipurpose plant — a review. Revista Brasileira de Farmacognosia 29: 77–10.

Nyvall R F. 1989. Diseases of Sugarcane. Field Crop Diseases Handbook. Springer, Boston, MA. 1-4757-5221-2_20

Rashmi M, Kushveer J S and Sarma V V. 2019. A worldwide list of endophytic fungi with notes on ecology and diversity. Mycosphere 10: 798–1079.

Romão-Dumaresq A S, Dourado M N, Fávaro L C dL, Mendes R, Ferreira A and Araújo, W L. 2016. Diversity of cultivated fungi associated with conventional and transgenic sugarcane and the interaction between endophytic Trichoderma virens and the host plant. PLoS One 11: e0158974. doi:10.1371/ journal.pone.0158974

Silva F A, Liotti R G, Boleti A, Reis É M, Passos M, Dos Santos E L, Sampaio O M, Januário A H, Branco C, Silva G, Mendonça E and Soares M A. 2018. Diversity of cultivable fungal endophytes in Paullinia cupana (Mart.) Ducke and bioactivity of their secondary metabolites. PloS One 13(4): e0195874.

Srivastava S, Gupta P S, Lal S and Sinha O K. 2017. Rapid identification of endophytic fungi of sugarcane Saccharum spp. hybrid) using PCR-RFLP of rDNA. Journal of Environmental Biology 38: 21–26.

Stuart R M, Romão A S, Pizzirani-Kleiner A A, Azevedo J L and Araújo W L. 2010. Culturable endophytic filamentous fungi from leaves of transgenic imidazolinone-tolerant sugarcane and its non-transgenic isolines. Archives of Microbiology 192: 307–13.

Tamura K and Nei M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512–26.

Tamura K, Stecher G and Kumar S. 2021. MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38(7): 3022–27.

Taylor J E, Hyde K D and Jones E B G. 1999. Endophytic fungi associated with the temperate palm, Trachycarpus fortunei within and outside its natural geographic range. New Phytologist 142: 335–46.

Wang Y, Guo L D and Hyde K D. 2005. Taxonomic placement of sterile morphotypes of endophytic fungi from Pinus tabulaeformis (Pinaceae) in northeast China based on rDNA sequences. Fungal Diversity 20: 235–60.

White T J, Bruns T, Lee S and Taylor J W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, pp. 315–22. Innis M A, Gelfand D H, Sninsky J J and White T J (Eds). Academic Press Inc, New York.

Verkley G J, Dukik K, Renfurm R, Göker M and Stielow J B. 2014. Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota). Persoonia 32: 25–51.









How to Cite

MALARVIZHI, K., MURALI, T. S., & KUMARESAN, V. (2022). First report of Paraphaeosphaeria angularis as endophyte in sugarcane (Saccharum officinarum) from India. The Indian Journal of Agricultural Sciences, 92(12), 1511–1513.