Identification of multiple abiotic stress tolerant donors for climate-resilient rice (Oryza sativa) development

Abstract views: 341 / PDF downloads: 321


  • KOUSHIK CHAKRABORTY ICAR-National Rice Research Institute, Cuttack, Odisha 753 006, India
  • SOMNATH ROY ICAR-National Rice Research Station, Hazaribag, Jharkhand
  • P JENA ICAR-National Rice Research Institute, Cuttack, Odisha 753 006, India
  • G K DASH MS Swaminathan School of Agriculture Centurion University of Technology and Management, Paralakhemundi, Gajapati, Odisha
  • M BARIK School of Applied Sciences, Centurion University of Technology and Management, Jatni, Odisha
  • MOTILAL BEHERA ICAR-National Rice Research Institute, Cuttack, Odisha 753 006, India
  • P SAR ICAR-National Rice Research Station, Hazaribag, Jharkhand
  • J SENAPATY ICAR-National Rice Research Institute, Cuttack, Odisha 753 006, India
  • M J BAIG ICAR-National Rice Research Institute, Cuttack, Odisha 753 006, India
  • PADMINI SWAIN ICAR-National Rice Research Institute, Cuttack, Odisha 753 006, India


Anaerobic germination, Drought, Early Vegetative Vigour, Submergence


The increasing occurrence of adverse weather events is continuously challenging agricultural production globally. The wide genetic base of rice (Oryza sativa L.) and the availability of a diverse set of germplasm can be explored to identify multiple abiotic stress-tolerant donors for rice improvement. Therefore, an experiment was conducted during 2018–21 at ICAR-National Rice Research Institute, Cuttack, Odisha to evaluate diverse rice germplasm (68 accessions) for vegetative stage drought and submergence stress and for their anaerobic germination potential. Under drought stress, 49 accessions showed a drought score (DS) of 1 indicating a tolerance response. The early vegetative score indicated that 58 accessions were highly vigorous, while the drought recovery score indicates that >90% of the genotypes showed good recovery after the drought cycle. The same genotypes showed a average survival rate (SR) of 48% under two weeks of complete submergence. Among them, 11 were found highly tolerant with >70% SR and 26 genotypes scored positive SUB1A locus by SNP-based functional marker AEX1. Nearly 50% of the genotypes scored positive for SUB1A, based on both AEX1 and Sub1A203 markers, which nearly matched the phenotyping result. Out of 68, only 4 accessions showed more than 50% germination under anaerobic conditions. A multivariate analysis showed that 11 genotypes had both drought and submergence tolerance, while 6 genotypes were having tolerance to drought and anaerobic germination. We found only 1 accession (IC516149) tolerant to all these 3 stresses which can be identified as a potential donor for multiple abiotic stresses.


Download data is not yet available.


Bin Rahman A N M R and Zhang J. 2022. The coexistence of flood and drought tolerance: An opinion on the development of climate-smart rice. Frontiers in Plant Science 13: 8–11.

Chakraborty K, Chattaopadhyay K, Nayak L, Ray S, Yeasmin L, Jena P, Gupta S, Mohanty S K, Swain P and Sarkar R K. 2019. Ionic selectivity and coordinated transport of Na+ and K+ in flag leaves render differential salt tolerance in rice at the reproductive stage. Planta 250: 1637–53.

Chakraborty K, Guru A, Jena P, Ray S, Guhey A, Chattopadhyay K and Sarkar R K. 2021. Rice with SUB1 QTL possesses greater initial leaf gas film thickness leading to delayed perception of submergence stress. Annals of Botany 127(2): 251–65.

Chakraborty K, Jena P, Mondal S, Dash G K, Ray S, Baig M J and Swain P. 2022a. Relative contribution of different members of OsDREB gene family towards osmotic stress tolerance in indica and japonica ecotypes of rice. Plant Biology 24: 356–66.

Chakraborty K, Mondal S, Jena P and Bhaduri D. 2022b. Tolerance mechanism of rice in submergence and stagnant flooding stress. Climate Resilient Technology, pp. 1–17. Publisher, NRRI, Cuttack, Odisha.

Colmer T D and Voesenek L A C J. 2009. Flooding tolerance: suites of plant traits in variable environments. Functional Plant Biology 36: 665–81.

Dien D C, Mochizuki T and Yamakawa T. 2019. Effect of various drought stresses and subsequent recovery on proline, total soluble sugar and starch metabolisms in Rice (Oryza sativa L.) varieties. Plant Production Science 22: 530–45.

Fukao T, Yeung E and Bailey Serres J. 2011. The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice. Plant Cell 23: 412–27.

Ikmal M A, Noraziyah A A S and Wickneswari R. 2021. Incorporating drought and submergence tolerance QTL in rice (Oryza sativa L.)-The effects under reproductive stage drought and vegetative stage submergence stresses. Plants Basel 10(2): 225.

IRRI. 2013. Standardization evaluation system for rice. International Rice Research Institute, Mahila, Phillippines 5: 18.

Ismail A M, Ella E S, Vergara G V and Mackill D J. 2009. The mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa). Annals of Botany 103: 197–209.

Kretzschmar T, Pelayo M A F, Trijatmiko K R, Gabunada L F M, Alam R, Jimenez R, Mendioro M S, Slamet Loedin I H, Sreenivasulu N, Bailey Serres J, Ismail A M, Mackill D J and Septiningsih E M. 2015. A trehalose-6-phosphate phosphatase enhances anaerobic germination tolerance in rice. Nature Plants 1: 15124.

Kumar A, Dixit S, Ram T, Yadaw R B, Mishra K K and Mandal N P. 2014. Breeding high-yielding drought-tolerant rice: genetic variations and conventional and molecular approaches. Journal of Experimental Botany 65(21): 6265–78.

Lonbani M and A Arzani. 2011. Morpho-physiological traits associated with terminal drought stress tolerance in triticale and wheat. Agronomy Research 9: 315–29.

Pathak H, Kumar M, Molla K A and Chakraborty K. 2021. Abiotic stresses in rice production: impact and management. Oryza 58(4): 103–25.

Septiningsih E M, Pamplona A M, Sanchez D L, Neeraja C N, Vergara G V, Heuer S, Ismail A M and Mackill D J. 2009. Development of submergence-tolerant rice cultivars: The Sub1 locus and beyond. Annals of Botany 103(2): 151–60.

Singh A, Singh Y and Mahato A K, Jayaswal P K, Singh S, Singh R, Yadav N, Singh A K, Singh P K, Siingh R, Kumar R, Septiningsih E M, Balyan H S, Singh N K and Rai V. 2020. Allelic sequence variation in the Sub1A, Sub1B and Sub1C genes among diverse rice cultivars and its association with submergence tolerance. Scientific Reports 10: 8621.

Verulkar S B, Mandal N P, Dwivedi J L, Singh B N, Sinha P K. Mahato R N, Swain P, Singh O N, Bose L K, Swain P, Robin S, Chandrababu R, Senthil S, Jain A, Shashidhar H E, Hittalmani S, Vera Cruz C, Paris T, Raman A, Haefele S, Serraj R, Atlin G and Kumar A. 2010. Breeding resilient and productive rice genotypes adapted to drought-prone rainfed ecosystems of India. Field Crops Research 117: 197–208.

Vijayan J, Senapati S, Ray S, Chakraborty K, Molla K A, Basak N, Pradhan B, Yeasmin L, Chattopadhyay K and Sarkar R K. 2018. Transcriptomic and physiological studies identify cues for germination stage oxygen deficiency tolerance in rice. Environmental and Experimental Botany 147: 234–48.









How to Cite

CHAKRABORTY, K., ROY, S., JENA, P., DASH, G. K., BARIK, M., BEHERA, M., SAR, P., SENAPATY, J., BAIG, M. J., & SWAIN, P. (2023). Identification of multiple abiotic stress tolerant donors for climate-resilient rice (Oryza sativa) development. The Indian Journal of Agricultural Sciences, 93(3), 258–262.