Effect of different biofertilizer strains on soil, leaf and yield parameters of Kinnow mandarin

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  • VIJAY Maharana Pratap Horticultural University, Karnal, Haryana
  • SOURABH ICAR-Central Arid Zone Research Institute, Jodhpur, Rajasthan
  • G S RANA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana
  • R P S DALAL Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana
  • RAKESH KUMAR Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana
  • PREETI ICAR-National Bureau of Plant Genetic Resources, New Delhi
  • AJAY KUMAR Krishi Vigyan Kendra, Jhajjar, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana



Azotobacter, Kinnow, Macro-micro nutrients, Microbial count, Pseudomonas


Present field study was carried out to investigate the effects of biofertilizer strains on soil, leaf and yield parameters of Kinnow zandarin (C. nobilis × C. deliciosa) during 2018–19 and 2019–20 at the experimental orchard of Department of Horticulture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana. The study revealed that soil, leaf nutrient status and yield parameters were significantly affected with the application of biofertilizers. There was synergistic influence of Azotobacter and PSB strains. The magnitude of increase with the application of different bio-strains was more pronounced when applied in combination with lower dose of fertilizers (75% RDF) than their combination with higher dose (100% RDF). 100% RDF + Azotobacter chroococcum Mac 27 + Pseudomonas P 36 (T7) was found most effective in enhancing soil macro-micro nutrients, leaf nutrient status and yield parameters.
Soil available N and P increased significantly in 0–15 cm soil layer during both years but in 15–30 cm soil layer, the enhancement was observed only during second year of investigation, however Fe, Zn and Mn showed significant improvement only in 0–15 cm soil layer during both years. Leaf N content increased in September, however no significant effect was observed in March values. Leaf N content showed fast increment as compared to leaf P content. 


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Anonymous. 2021. Horticulture Department, Government of Haryana. http://www. hortharyana. gov. in/en/statistical-data.

Aseri G K and Tafardar J C. 2006. Fluorescein diacetate: A potential biological indicator for arid soils. Arid Land Resourse Management 20(2): 87–99. DOI: https://doi.org/10.1080/15324980500544473

Athani S I, Prabhuraj H S, Ustad A I, Swamy G S K, Patil P B and Kotikal Y K. 2007. Effect of organic and inorganic fertilizers on growth, leaf, major nutrient and chlorophyll content and yield of guava cv. Sardar. Acta Horticulturae 735: 351–56. DOI: https://doi.org/10.17660/ActaHortic.2007.735.50

Dick W A. 1980. Phosphatases in the soil environment. Retrospective Theses and Dissertations 7370. http://lib. dr. iastate. edu/rtd/7370

Dutta P and Kundu S. 2012. Effect of bio-fertilizers on nutrient status and fruit quality of Himsagar mango grown in new alluvial zones of West Bengal. Journal of Crop Weed 8(1): 72–74.

Godage S S, Parekh N S and Nehete D S. 2013. Influence of bio-fertilizers and chemical fertilizers on growth, flowering and fruit characters of guava (Psidium guajava L.) cv. Allahabad Safeda. International Journal of Agricultural Sciences 9(1): 309–13.

Gogoi D, Kotoky U and Hazarika S. 2004. Effect of biofertilizers on productivity and soil characteristics in banana. Indian Journal of Horticulture 61(4): 354–56.

Hanway J J and Heidel H H. 1952. Soil analysis methods as used in Iowa State College, Soil Testing Laboratory. Iowa State College Bulletin 57: 1–131.

Hoda M M, Faten A A and Azza A M A. 2013. Effect of Magnetite and some biofertilizers application on growth and yield of Valencia Orange trees under El-Bustan condition. Natural Science 11(6): 46–61.

Jackson M L. 1967. Soil Chemical Analysis, pp 205. Prentice Hall of India Pvt. Ltd., New Delhi.

Kumar A and Tripathi V K. 2020. Effect of Azotobacter, PSB and vermicompost on growth, flowering, yield and quality of strawberry (Fragaria × ananassa Duch.) cv. Chandler. Progressive Horticulture 52(2): 157–61. DOI: https://doi.org/10.5958/2249-5258.2020.00022.6

Li R, Pang Z, Zhou Y, Fallah N, Hu C, Lin W and Yuan Z. 2020 Metagenomic analysis exploring taxonomic and functional diversity of soil microbial communities in sugarcane fields applied with organic fertilizer. BioMed Research International 2020: 9381506. DOI: https://doi.org/10.1155/2020/9381506

Lindsay W L and Norvell W A. 1978. Development of a DTPA soil test for zinc, iron, manganeseand copper. Soil Science Society of American Journal 42(3): 421–28. DOI: https://doi.org/10.2136/sssaj1978.03615995004200030009x

Marathe R A and Bharambe P R. 2007. Growth, yield and quality of sweet orange cv. Mosambi in response to INM in vertisols of Central India. Indian Journal of Horticulture 64(3): 274–77.

Medhi B K, Saikia A K, Bora S C, Hazarika T K and Barbora A C. 2007. Integrated use of concentrated organic manures, biofertilizers and inorganic NPK on yield, quality and nutrient content of Khasi Mandarin (Citrus reticulate Blanco.). Indian Journal of Agriculture Research 41(4): 235–41.

Meena H R, Somasundaram J, Kaushik R A, Sarolia D K, Singh R K and Meena G L. 2019. Integrated nutrient management affects fruit yield of sapota (Achras zapota L.) and nutrient availability in a Vertisol. Communications in Soil Science and Plant Analysis 50(22): 2848–63. DOI: https://doi.org/10.1080/00103624.2019.1689248

Mishra A N and Tripathi V K. 2011. Influence of different levels of Azotobacter, PSB alone and in combination on vegetative growth, flowering, yield and quality of strawberry cv. Chandler. International Journal of Applied Agricultural Research 6(3): 203–10.

Morselli T B G A, Sallis M D G, Terra S and Fernandes H S. 2004. Response of lettuce to application of vermicompost. Revista Cientifica Rural 9: 1–7.

Olsen S R, Cole C V, Watanabe F S and Dean L A. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circ. 939. Washington, D. C.

Pasricha N S and Sarkar A K. 2002. Secondary nutrients. Fundamentals of Soil Science, pp. 381–89. Indian Society of Soil Science, New Delhi.

Patel D R and Naik A G. 2010. Effect of pre-harvest treatment of organic manures and inorganic fertilizers on post-harvest shelf-life of sapota cv. Kalipatti. Indian Journal of Horticulture 67(3): 381–86.

Piper C S. 1966. Soil and Plant Analysis. Hans Publication, Bombay, India.

Powar C B and Daginawala H F. 2004. Nitrogen Fixation. General Microbiology, Vol. 1, Chapter 23, p. 607. Himalaya Publishing House, Mumbai.

Ram R A, Bhriguvanshi S R and Pathak R K. 2007. Integrated plant nutrient management in guava (Psidium guajava L.) cv. Sardar. Acta Horticulturae 735: 345–50. DOI: https://doi.org/10.17660/ActaHortic.2007.735.49

Sau S, Mandal P, Sarkar T, Das K and Datta P. 2017. Influence of bio-fertilizer and liquid organic manures on growth, fruit quality and leaf mineral content of mango cv. Himsagar. Journal of Crop and Weed 13(1): 132–36.

Singh A and Singh J N. 2009. Effect of bio-fertilizers and bio-regulators on growth, yield and nutrient status of strawberry cv. Sweet Charlie. Indian Journal of Horticulture 66(2): 220–24.

Sourabh, Sharma J R, Baloda S, Kumar R, Sheoran V, Vijay and Saini H. 2018. Response of organic amendments and biofertilizers on growth and yield of guava during rainy season. Journal of Pharmacognosy and Phytochemistry 7(6): 2692–95.

Subbiah B V and Asija J S. 1956. A rapid procedure for the estimation of available nitrogen in the soil. Current Science 25: 259–60.

Tripathi V K, Mishra A N, Kumar S and Tiwari B. 2014. Efficacy of Azotobacter and PSB on vegetative growth, flowering, yield and quality of strawberry cv. Chandler. Progressive Horticulture 46(1): 48–53. DOI: https://doi.org/10.5958/2249-5258.2016.00009.9









How to Cite

VIJAY, SOURABH, RANA, G. S., DALAL, R. P. S., KUMAR, R., PREETI, & KUMAR, A. (2023). Effect of different biofertilizer strains on soil, leaf and yield parameters of Kinnow mandarin. The Indian Journal of Agricultural Sciences, 93(4), 392–396. https://doi.org/10.56093/ijas.v93i4.134247