Assessment of male date palm (Phoenix dactylifera) genotypes based on morphological and chemotypic attributes
136 / 53
Keywords:
Biochemical potential, Date palm, Morphological diversity, PollinisersAbstract
Date palm (Phoenix dactylifera L.) is a perennial dioecious plant of hot arid ecosystem, commercially cultivated for edible fruits. Non-synchronous flowering phenomenon in date palm is one of the major challenges, therefore, artificial pollination is essentially required for successful fruit setting. Very scanty and scattered information to identify the superior male date palm genotypes as potential pollinisers based on morpho-biochemical characterisation is available. Therefore, the present study was performed to characterise nine male date palm genotypes based on phenotypic and biochemical parameters during 2020–22 at ICAR-Central Institute for Arid Horticulture, Bikaner, Rajasthan. The carpological and morphological measures of leaf, spathe and inflorescence showed significant variation. The results revealed that CIAH-DP-M8 exhibited the maximum leaf length (400 cm) whereas, the CIAH-DP-M5 exhibited the shortest leaves (250.0 cm). The CIAH-DP-M1 showed superiority in all the recorded parameters related to the inflorescence among the genotype studied. Similarly, in the biochemical parameters also, it was significantly superior in total phenolic content (11.90 mg GAE/g), total antioxidant capacity (16.35 mg AAE/g DW), flavonoids (0.496 mg E.Cat./g DW) and total sugar content (80.90 mg/g) which proved its potential for quality pollen grains production of date palm. The characterised promising male date palm genotype in the present study could serve as potential polliniser in western India.
Downloads
References
Abu-Zahra T R and Shatnawi M A. 2019. New pollination technique in date palm (Phoenix dactylifera L.) cv. Barhee and Medjool under Jordan valley conditions. AmericanEurasian Journal of Agricultural and Environmental Sciences 19: 7–42.
Abou-Zeid, H M, M A Shiha and Shehata A A. 2019. Comparative study of pollen grains morphology and phytochemical constituents of some Saudi Arabian date palm (Phoenix dactylifera L.) cultivars. International Journal of Current Microbiology and Applied Sciences 8: 2800–09. DOI: https://doi.org/10.20546/ijcmas.2019.807.350
Ahmed M V, Bouna Z E, Lemine F M, Djeh T K, Mokhtar T and Salem A O. 2011. Use of multivariate analysis to assess phenotypic diversity of date palm (Phoenix dactylifera L.) cultivars. Scientia Horticulturae 127: 367–71. https://doi. org/10.1016/j.scienta.2010.11.011 DOI: https://doi.org/10.1016/j.scienta.2010.11.011
Al-Mssallem I S, Hu S, Zhang X, Lin Q, Liu W, Tan J, Yu X, Liu J, Pan L, Zhang T, Yin Y, Xin C, Wu H, Zhang G, Ba- Abdullah M M, Huang D, Fang Y, Alnakhali Y O, Jia S, Yin A, Alhuzimi E M, Alsaihati B A, Al-Owayyed S A, Zhao D, Zhang S, Al-Otaibi N A, Sun G, Majrashi M A, Li F, Tala, Wang J, Yun Q, Alnassar N A, Wang L, Yang M, Al-Jelaify R F, Liu K, Gao S, Chen K, Alkhaldi S R, Liu G, Zhang M, Guo H and Yu J. 2013. Genome sequence of the date palm Phoenix dactylifera L. Nature Communications 4: 1–9. https:// doi.org/10.1038/ncomms3274 DOI: https://doi.org/10.1038/ncomms3274
Al-Shahib W and Marshall R J. 2003. The fruit of the date palm: Its possible use as the best food for the future. International Journal of Food Sciences and Nutrition 54: 247–59. https:// doi.org/10.1080/09637480120091982 DOI: https://doi.org/10.1080/09637480120091982
Apak R, Guclu K, Ozyurek M and Karademir S E. 2004. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry 52: 7970–81. https://doi. org/10.1021/jf048741x DOI: https://doi.org/10.1021/jf048741x
Arafat K H, A M Mohamad and Elsharabasy S. 2013. Influence of environmental conditions, salinity and root exudates on incidence and disease severity of Lasiodiplodia theobromae that caused root rot of date palm offshoots and biocontrolling. Journal of Biological Chemistry and Environmental Sciences 8: 73–91.
Berwal M K, S M Haldhar, Chet Ram, S Shil and Gora J S. 2021. Effect of extraction solvent on total phenolics, flavonoids and antioxidant capacity of flower bud and foliage of Calligonum polygonoides (L.). Indian Journal of Agricultural Biochemistry 34(1): 61–67. https://doi.org/10.5958/0974-4479.2021.00008.3 DOI: https://doi.org/10.5958/0974-4479.2021.00008.3
Carillo P and Gibon Y. 2011. PROTOCOL: Extraction and determination of proline. https://prometheusprotocols.net/ function/tissue-chemistry/primary-metabolites/extraction-and- determination-of-proline/
Chao C T and Krueger R R. 2007. The date palm (Phoenix dactylifera L.): Overview of biology, uses and cultivation. Hort Science 42: 1077–82. DOI: 10.21273/HORTSCI.42.5.1077 DOI: https://doi.org/10.21273/HORTSCI.42.5.1077
Dubois M, Gilles K A, Hamilton J K, Rebers P A and Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistery 28: 350–56. https://doi.org/10.1021/ac60111a017 DOI: https://doi.org/10.1021/ac60111a017
El Kadri N, Mimoun M B and Hormaza J I. 2019. Genetic diversity of Tunisian male date palm (Phoenix dactylifera L.) genotypes using morphological descriptors and molecular markers. Scientia Horticulturae 253: 24–34. https://doi.org/10.1016/j. scienta.2019.04.026 DOI: https://doi.org/10.1016/j.scienta.2019.04.026
Elhoumaizi M A, Saaidi M, Oihabi A and Cilas C. 2002. Phenotypic diversity of date-palm cultivars (Phoenix dactylifera L.) from Morocco. Genetic Resources and Crop Evolution 49: 483–90. https://doi.org/10.1023/A:1020968513494 DOI: https://doi.org/10.1023/A:1020968513494
Elsafy M, Gustavsson L and Mujaju C. 2015. Phenotypic diversity of date palm cultivars (Phoenix dactylifera L.) from Sudan estimated by vegetative and fruit characteristics. International Journal of Biodiversity: 610391. http://dx.doi.org/10.1155/2015/610391 DOI: https://doi.org/10.1155/2015/610391
Evanno Guillaume Sebastien R and Jerome G. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular ecology 14: 2611–20. https://doi.org/10.1111/j.1365-294X.2005.02553.x DOI: https://doi.org/10.1111/j.1365-294X.2005.02553.x
FAO. 2020. The State of Food and Agriculture 2020. Overcoming Water Challenges in Agriculture. Food and Agriculture Organisation of the United Nations, Rome. https://openknowledge.fao.org/handle/20.500.14283/cb1447en
Farouk A, Metwaly A and Mohsen M. 2015. Chemical composition and antioxidant activity of date palm pollen grains (Phoenix dactylifera L. Palmae) essential oil for Siwe cultivar cultivated in Egypt. Middle East Journal Applied Science 5: 945–49.
Hammadi H, Mokhtar R E, Mokhtar E and Ali F. 2009. New approach for the morphological identification of date palm (Phoenix dactylifera L.) cultivars from Tunisia. Pakistan Journal of Botany 41: 2671–81.
Hassan Hazem M M. 2011. Chemical composition and nutritional value of palm pollen grains. Global Journal of Biotechnology Biochemistry 6: 1–7.
Johnson D V, Al-Khayri J M and Jain S M. 2013. Seedling date palms (Phoenix dactylifera L.) as genetic resources. Emirates Journal of Food and Agriculture 24: 809–30. https://doi.org/10.9755/ejfa.v25i11.16497 DOI: https://doi.org/10.9755/ejfa.v25i11.16497
Medini F, Fellah H, Ksouri R and Abdelly C. 2014. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taiba University for Science 8: 216–24. https://doi.org/10.1016/j.jtusci.2014.01.003 DOI: https://doi.org/10.1016/j.jtusci.2014.01.003
Nixon R W. 1928. Immediate influence of pollen in determining the size and time of ripening of the fruit of the date palm. Journal of Heredity 19: 241–55. DOI: https://doi.org/10.1093/oxfordjournals.jhered.a102993
Raza M K, Jaskani M J, Naqvi S A and Awan F S. 2020. Exploitation of phenotypic diversity in male accessions of date palm (Phoenix dactylifera) and its use in germplasm conservation. International Journal of Agricultural Biology 24: 133–44. DOI: https://doi.org/10.17957/IJAB/15.1417
Saber S F. 2016. Estimate proline and carotenoids in pollen grains of some varieties of date palm Phoenix dactylifera L. Thi-Qar University Journal for Agricultural Researches 5: 287–98.
Shah J J. 2014. Date palm cultivation in India: An overview of activities. Emirates Journal of Food and Agriculture 15: 987–99. DOI: https://doi.org/10.9755/ejfa.v26i11.18986
Shahsavar A R and Shahhosseini A. 2022. The metaxenia effects of diferent pollen grains on secondary metabolites enzymes and sugars of ‘Piarom’ date palm fruit. Scientific Reports 12: 10058. https://doi.org/10.1038/s41598-022-14373-w DOI: https://doi.org/10.1038/s41598-022-14373-w
Simozrag A, Chala A, Djerouni A and Bentchikou M E. 2016. Phenotypic diversity of date palm cultivars (Phoenix dactylifera L.) from Algeria. Gayana Botanica 73: 42–53. http://dx.doi. org/10.4067/S0717-66432016000100006 DOI: https://doi.org/10.4067/S0717-66432016000100006
Somogyi M. 1952. Estimation of sugars by colorimetric method. Journal of Biological Chemistry 200: 245.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 The Indian Journal of Agricultural Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.